Regulatory mechanism of flagellar and ciliary movement via calcium signaling

• Project/Area Number: 21770075
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: University of Tsukuba
• Principal Investigator: SHIBA Kogiku University of Tsukuba, 大学院・生命環境科学研究科, 助教 (70533561)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 動物生理科学 / 細胞・組織 / 生理学 / 動物 / 鞭毛・繊毛 / 生殖

Research Abstract

To understand how flagella and cilia respond to external stimuli and instantaneously modify their movement, regulatory mechanisms of flagellar and ciliary movement via calcium signaling during sperm chemotactic behavior and motility activation in Ciona intestinalis and ciliary reversal movement in sea urchin larvae were analyzed. Sensory mechanism of sperm chemoattractant, calcium-independent regulatory mechanism of flagellar asymmetry, cAMP signaling in sperm motility activation and calcium influx during ciliary reversal movement were revealed by these studies.

Research Products

• [Journal Article] Mechanism regulating Ca(2+)-dependent mechanosensory behaviour in sea urchin spermatozoa (2011)
  • Author(s): Kambara, Y., Shiba, K., Yoshida, M., Sato, C., Kitajima, K., Shingyoji, C.
  • Journal Title: Cell Structure and Function 36(1) Pages: 69-82
  • NAID: 130004137565
  • Peer Reviewed
• [Journal Article] Mechanism regulating Ca(2+)-dependent mechanosensory behaviour in sea urchin spermatozoa (2011)
  • Author(s): Kambara, Y., Shiba, K., Yoshida, M., Sato, C., Kitajima, K., Shingyoji, C.
  • Journal Title: Cell Struct Funct. Volume: 36(1) Pages: 69-82
  • NAID: 130004137565
  • Peer Reviewed
• [Journal Article] ankAT-1 is a novel gene mediating the apical tuft formation in the sea urchin embryo (2010)
  • Author(s): Yaguchi, S., Yaguchi, J., Wei, Z., Shiba, K., Angerer, L.M., Inaba, K.
  • Journal Title: Developmental Biology 348(1) Pages: 67-75
  • NAID: 120007137750
  • Peer Reviewed
• [Journal Article] ankAT-1 is a novel gene mediating the apical tuft formation in the sea urchin embryo (2010)
  • Author(s): Yaguchi, S., Yaguchi, J., Wei, Z., Shiba, K., Angerer, L.M., Inaba, K.
  • Journal Title: Developmental Biology Volume: 348(1) Pages: 67-75
  • NAID: 120007137750
  • Peer Reviewed
• [Presentation] ウニ幼生頂毛の構造とタンパク質組成について (2010)
  • Author(s): 金銀華, 谷口俊介, 柴小菊, 稲葉一男
  • Organizer: 第5回鞭毛・ダイニン研究会
  • Place of Presentation: 東京大学本郷キャンパス
  • Year and Date: 2010-03-23
• [Presentation] 遊泳中のウニ精子における鞭毛運動のカルシウム制御 (2010)
  • Author(s): 蒲原祐花, 柴小菊, 北島健, 真行寺千佳子
  • Organizer: 第5回鞭毛・ダイニン研究会
  • Place of Presentation: 東京大学本郷キャンパス
  • Year and Date: 2010-03-23
• [Presentation] カタユウレイボヤにおける自己非自己識別分子とそれを介するカルシウムシグナルの解析 (2010)
  • Author(s): 齋藤貴子、柴小菊、稲葉一男、原田淑人、山田力志、澤田均
  • Organizer: BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] NCS ファミリータンパク質カラクシンのホヤ精子走化性における機能 (2010)
  • Author(s): 水野克俊, 柴小菊, 稲葉一男
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学教養学部駒場キャンパス
• [Presentation] ウニ胚頂毛に存在するグルタチオンS-トランスフェラーゼの胚繊毛運動における役割 (2010)
  • Author(s): 金銀華, 柴小菊, 谷口俊介, 稲葉一男
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学教養学部駒場キャンパス
• [Presentation] カタユウレイボヤ初期胚における繊毛形成とその時空間的パターン解析 (2010)
  • Author(s): 谷口順子, 紺野在, 金銀華, 柴小菊, 谷口俊介, 稲葉一男
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学教養学部駒場キャンパス
• [Presentation] Characterization of adenylylcyclase involved in flagellar motility activation in Ciona sperm. (2010)
  • Author(s): Shiba, K.Hozumi, A., Inaba, K.
  • Organizer: FASEB Summer Research Conferences : The Biology of Cilia and Flagella.
  • Place of Presentation: Saxtons River, VT, USA.
• [Presentation] NCS family protein calaxin is a calcium dependent dynein modulator responsible for sperm chemotaxis. (2010)
  • Author(s): Mizuno, K., Shiba, K., Inaba, K.
  • Organizer: FASEB Summer Research Conferences : The Biology of Cilia and Flagella.
  • Place of Presentation: Saxtons River, VT, USA.
• [Presentation] Characterization of adenylylcyclase involved in sperm motility activation in Ciona intestinalis. (2010)
  • Author(s): Shiba, K., Hozumi, A., Inaba, K.
  • Organizer: 11^<th> International Symposium on Spermatology
  • Place of Presentation: Okinawa ConventionCenter.
• [Presentation] Function of NCS family protein calaxin in the regulation of sperm flagellar waveform. (2010)
  • Author(s): Mizuno, K., Shiba, K., Inaba, K.
  • Organizer: 11^<th> International Symposium on Spermatology
  • Place of Presentation: Okinawa ConventionCenter.
• [Presentation] Ca2+-dependent Regulation of Sea Urchin Sperm Swimming Before and After Mechanical Stimulation. (2010)
  • Author(s): Kambara, Y., Shiba, K., Yoshida, M., Sato, C. Kitajima, K., Shingyoji, C.
  • Organizer: 11^<th> International Symposium on Spermatology
  • Place of Presentation: Okinawa ConventionCenter.
• [Presentation] 新しい精子遊泳運動動態の判別法による走化性反応の検出 (2010)
  • Author(s): 宮代大輔, 柴小菊, 上村慎治, 馬場昭次, 吉田学
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 中央大学理工学部
• [Presentation] ホヤ精子運動活性化に関与するアデニル酸シクラーゼの同定 (2010)
  • Author(s): 柴小菊, 保住暁子, 稲葉一男
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 中央大学理工学部
• [Presentation] Characterization of adenylylcyclase involved in sperm motility activation in Ciona intestinalis (2010)
  • Author(s): Kogiku Shiba, Akiko Hozumi, Kazuo Inaba
  • Organizer: 11^<th> International Symposium on Spematology
  • Place of Presentation: 沖縄コンベンションセンター、沖縄
• [Presentation] Function of NCS family protein calaxin in the regulation of sperm flagellar waveform (2010)
  • Author(s): Katsutoshi Mizuno, Kogiku Shiba, Kazuo Inaba
  • Organizer: 11^<th> International Symposium on Spermatology
  • Place of Presentation: 沖縄コンベンションセンター、沖縄
• [Presentation] Ca2+-dependent Regulation of Sea Urchin Sperm Swimming Before and After Mechanical Stimulation (2010)
  • Author(s): Yuka Kambara, Kogiku Shiba, Chihiro Sato, Ken Kitajima, Chikako Shingyoji
  • Organizer: 11^<th> International Symposium on Spermatology
  • Place of Presentation: 沖縄コンベンションセンター、沖縄
• [Presentation] Characterization of adenylylcyclase involved in flagellar motility activation in Ciona sperm (2010)
  • Author(s): Kogiku Shiba, Akiko Hozumi, Kazuo Inaba
  • Organizer: FASEB Summer Research Conferences : The Biology of Cilia and Flagella
  • Place of Presentation: Saxtons River, VT, USA
• [Presentation] NCS family protein calaxin is a calcium dependent dynein modulator responsible for sperm chemotaxis (2010)
  • Author(s): Katsutoshi Mizuno, Kogiku Shiba, Kazuo Inaba
  • Organizer: FASEB Summer Research Conferences : The Biology of Cilia and Flagella
  • Place of Presentation: Saxtons River, VT, USA
• [Presentation] NCSファミリータンパク質カラクシンのホヤ精子走化性における機能 (2010)
  • Author(s): 水野克俊, 柴小菊, 稲葉一男
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学駒場キャンパス、東京
• [Presentation] ウニ胚頂毛に存在するグルタチオンS-トランスフェラーゼの胚繊毛運動における役割 (2010)
  • Author(s): 金銀華, 柴小菊, 谷口俊介, 稲葉一男
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学駒場キャンパス、東京
• [Presentation] カタユウレイボヤ初期胚における繊毛形成とその時空間的パターン解析 (2010)
  • Author(s): 谷口順子, 紺野在, 金銀華, 柴小菊, 谷口俊介, 稲葉一男
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学駒場キャンパス、東京
• [Presentation] ホヤ精子運動活性化に関与するアデニル酸シクラーゼの同定 (2010)
  • Author(s): 柴小菊・保住暁子・稲葉一男
  • Organizer: 生体運動研究合同班会議
  • Place of Presentation: 中央大学理工学部・大講義室, 東京
• [Presentation] Characterization of adenylylcyclase involved in Flagellar motility activation in Ciona sperm. (2009)
  • Author(s): Shiba, K., Hozumi, A., Inaba, K.
  • Organizer: International Workshop Dynein 2009.
  • Place of Presentation: Kobe Fashion Museum.
• [Presentation] Roles of an axonemal Ca^<2+> binding protein, calaxin, in the regulation of sperm flagellar waveforms. (2009)
  • Author(s): Mizuno, K., Shiba, K., Inaba, K.
  • Organizer: International Workshop Dynein 2009.
  • Place of Presentation: Kobe Fashion Museum.
• [Presentation] ホヤ精子走化性実測データに基づく精子誘引物質感知機構のシミュレーション解析Chemoattractant Sensing Mechanisms in Sperm : Experimental and Simulation Study (2009)
  • Author(s): Miyashiro, D., Shiba, K., Kamimura, S., Baba, S.A., Yoshida, M.
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島文理大学/アスティとくしま
• [Presentation] 精子鞭毛波形調節におけるNCS ファミリータンパク質カラクシンの機能Function of NCS family protein calaxin in the regulation of sperm flagellar waveform (2009)
  • Author(s): Mizuno, K., Shiba, K., Inaba, K.
  • Organizer: 日本生物物理学会第47回年会
  • Place of Presentation: 徳島文理大学/アスティとくしま
• [Presentation] A novel gene mediating formation of apical tuft formation in the sea urchin, Hemicentrotus pulcherrimus. (2009)
  • Author(s): Yaguchi, J., Wei, Z., Shiba, K., Inaba, K., Yaguchi, S.
  • Organizer: Sea urchin meeting
  • Place of Presentation: Woods Hole, MA, USA.
• [Presentation] ホヤ精子鞭毛カラクシンの鞭毛運動における機能 (2009)
  • Author(s): 水野克俊, 柴小菊, 稲葉一男
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡グランシップ
• [Presentation] ホヤ精子運動活性化に関与するアデニル酸シクラーゼの同定 (2009)
  • Author(s): 柴小菊, 保住暁子, 稲葉一男
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡グランシップ
• [Presentation] Real-time imaging of intracellular Ca^<2+> and flagellar waveforms during sperm chemotaxis in the ascidian, Ciona intestinalis. (2009)
  • Author(s): Maruyama, K., Shiba, K., Baba, S.A., Yoshida, M.
  • Organizer: Gordon Research Conference : Fertilization and the Activation of Development
  • Place of Presentation: Plymouth, USA
• [Presentation] Experimental and simulation study on sperm chemotaxis in the ascidian, Ciona intestinalis. (2009)
  • Author(s): Shiba, K., Miyashiro, D., Kamimura, S., Baba, S.A., Yoshida, M.
  • Organizer: Gordon Research Conference : Fertilization and the Activation of Development
  • Place of Presentation: Plymouth, USA
• [Presentation] Sperm chemotactic behavior and Ca2+ dynamics of the ascidian, Ciona intestinalis. (2009)
  • Author(s): Shiba, K., Baba, S.A., Yoshida, M.
  • Organizer: The 5th International Tunicate Meeting.
  • Place of Presentation: Okinawa Industry Support Center.
• [Presentation] Sperm Chemotactic Behavior and Ca2+ Dynamics of the Ascidian, Ciona intestinalis (2009)
  • Author(s): Kogiku Shiba, Shoji A Baba, Manabu Yoshida
  • Organizer: The 5th International Tunicate Meeting
  • Place of Presentation: The Okinawa Industrial Supporting Center, Naha-city, Okinawa, Japan
• [Presentation] Experimental and simulation study on sperm chemotaxis in the ascidian, Ciona intestinalis (2009)
  • Author(s): Kogiku Shiba, Daisuke Miyashiro, Shinji Kamimura, Shoji A Baba, Manabu Yoshida
  • Organizer: Gordon Research Conference : Fertilization and the Activation of Development
  • Place of Presentation: Holderness School, Plymouth, USA
• [Presentation] Role of Ca2+ in chemotactic behavior of ascidian sperm (2009)
  • Author(s): Kotetsu Maruyama, Kogiku Shiba, Shoji A Baba, Manabu Yoshida
  • Organizer: Gordon Research Conference : Fertilization and the Activation of Development
  • Place of Presentation: Holderness School, Plymouth, USA
• [Presentation] ホヤ精子運動活性化に関与するアデニル酸シクラーゼの同定 (2009)
  • Author(s): 柴小菊・保住暁子・稲葉一男
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡グランシップ, 静岡
• [Presentation] ホヤ精子鞭毛カラクシンの鞭毛運動における機能 (2009)
  • Author(s): 水野克俊, 柴小菊, 稲葉一男
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡グランシップ, 静岡
• [Presentation] Characterization of adenylylcyclase involved in Flagellar motility activation in Ciona sperm (2009)
  • Author(s): 柴小菊・保住暁子・稲葉一男
  • Organizer: The International Workshop Dynein 2009
  • Place of Presentation: Kobe Fashion Museum, Orbis Hall, Kobe, JAPAN
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