The distribution and metabolism of D-arginine in animals.

• Project/Area Number: 21770080
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: Kochi University
• Principal Investigator: UDA Kouji 高知大学, 教育研究部・自然科学系, 助教 (10448392)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: D-アミノ酸 / アルギニンキナーゼ / D-アルギニン / フォスファゲンキナーゼ

Research Abstract

Recent research has demonstrated that D-amino acids are widely distributed in living organisms, from bacteria to mammals, and play a key role in specific physiological functions, although the full picture is not yet known. In this study, we isolated D-arginine and arginine kinase from various animals. Furthermore, we propose a unique substrate-recognition system for Sabellastarte arginine kinase.

Research Products

• [Journal Article] Complete mitochondrial genomes of two Japanese precious corals, Paracorallium japonicum and Corallium konojoi(Cnidaria, Octocorallia, Coralliidae) (2011)
  • Author(s): Uda K., Komeda Y., Koga K., Fujita T., Iwasaki N., Suzuki, T
  • Journal Title: Notable differences in gene arrangement Volume: 476 Pages: 27-37
  • Peer Reviewed
• [Journal Article] Molecular and catalytic properties of an arginine kinase from the nematode Ascar is suum (2011)
  • Author(s): Nagataki M.
  • Journal Title: J.Helminthol. Volume: 25 Issue: 3 Pages: 1-11
  • DOI: 10.1017/s0022149x11000381
  • Peer Reviewed
• [Journal Article] Molecular characterization of a cDNA-derived phosphagen kinases from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni (2011)
  • Author(s): Agatsuma T., Fukunaga S., Jarilla R.B., Natataki M.
  • Journal Title: Med.Entomol.Zool. Volume: Vol.62 Pages: 1-11
  • Peer Reviewed
• [Journal Article] Complete mitochondrial genomes of two Japanese precious corals, Paracorallium japonicum and Corallium konojoi (Cnidaria, Octocorallia, Coralliidae) : Notable differences in gene arrangement (2011)
  • Author(s): Uda K., Komeda Y., Koga K., Fujita T., Iwasaki N., Suzuki, T
  • Journal Title: Gene Volume: Vol.476 Issue: 1-2 Pages: 27-37
  • DOI: 10.1016/j.gene.2011.01.019
  • Peer Reviewed
• [Journal Article] Arginine kinase from the tardigrade, Macrobiotus occidentalis. Molecular cloning, phylogenetic analysis and enzymatic properties (2010)
  • Author(s): Uda K., Ishida M., Matsui T., Suzuki, T
  • Journal Title: Zoological Science Volume: 27 Pages: 795-803
  • NAID: 40017358645
  • Peer Reviewed
• [Journal Article] Identification of key amino acid residues distinguishing chiral guanidino substrates(D-and L-arginine) in Sabellastarte arginine kinase (2010)
  • Author(s): Uda K., Matumoto, A., Suzuki, T
  • Journal Title: J. Molecular Catalysis Volume: 64 Pages: 75-80
  • Peer Reviewed
• [Journal Article] Identification of key amino acid residues distinguishing chiral guanidino substrates(D- and L-arginine)in Sabellastarte arginine kinase. (2010)
  • Author(s): Uda K.
  • Journal Title: J.Molecular Catalysis 64 Pages: 75-80
  • Peer Reviewed
• [Presentation] ミダレウミシダのフォスファゲンキナーゼ遺伝子の単離とその酵素活性 (2011)
  • Author(s): 宇田幸司
  • Organizer: 日本動物学会
  • Place of Presentation: 大雪クリスタルホール(北海道)
  • Year and Date: 2011-09-22
• [Presentation] オウムガイ・アルギニンキナーゼにおける基質結合時の構造変化について (2010)
  • Author(s): 松本保
  • Organizer: 日本動物学会第81回東京大会
  • Place of Presentation: 東京大学(東京)
  • Year and Date: 2010-09-25
• [Presentation] クマムシのアルギニンキナーゼ遺伝子の単離とその酵素活性 (2009)
  • Author(s): 宇田幸司
  • Organizer: 日本動物学会
  • Place of Presentation: 静岡グランシップ(静岡県)
  • Year and Date: 2009-09-19
• [Presentation] クマムシのアルギニンキナーゼ遺伝子の単離とその酵素活性 (2009)
  • Author(s): 宇田幸司
  • Organizer: 日本動物学会 第80回静岡大会
  • Place of Presentation: 静岡グランシップ(静岡県)
  • Year and Date: 2009-09-19