The molecular dynamics study of an oxytocin/vasopressin superfamily peptide in the ascidian, Ciona intestinalis.

• Project/Area Number: 21770082
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Animal physiology/Animal behavior
• Research Institution: 公益財団法人サントリー生命科学財団 (2010); Suntory Institute for Bioorganic Research (2009)
• Principal Investigator: KAWADA Tsuyoshi 公益財団法人サントリー生命科学財団, 生物有機科学研究所, 研究員 (90300821)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 神経生物 / オキシトシン / バソプレシン

Research Abstract

Ci-VP is an oxytocin/vasopressin like peptide identified from an ascidian, Ciona intestinalis. We have established a transgenic line of the ascidian that expresses a fluorescent protein with Ci-VP gene promoter region. The imaging analysis with the fluorescent protein demonstrated that Ci-VP gene is transcribed not only in the brain ganglion but also in the nerve fibers and peripheral neural cells.

Research Products

• [Journal Article] Peptidomic Analysis of the Central Nervous System of the Protochordate, Ciona intestinalis : Homologs and Prototypes of Vertebrate Peptides and Novel Peptides. (2011)
  • Author(s): Kawada T., Ogasawara M., Sekiguchi T., Aoyama M., Hotta K., Oka K., Satake H.
  • Journal Title: Endocrinology 152 Pages: 2416-2427
  • Peer Reviewed
• [Journal Article] Expression of neuropeptide-and hormone-encoding genes in the Ciona intestinalis larval brain. (2011)
  • Author(s): Hamada M., Shimozono N., Ohta N., Satou Y., Horie T., Kawada T., Satake H., Sasakura Y., Satoh N.
  • Journal Title: Dev Biol. 352 Pages: 202-214
  • Peer Reviewed
• [Journal Article] Peptidomic analysis of the central nervous system of the protochordate, Ciona intestinalis : homologs and prototypes of vertebrate peptides and novel peptides (2011)
  • Author(s): Tsuyoshi Kawada
  • Journal Title: Endocrinology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Neuropeptides, hormone peptides, and their receptors in Cionaintestinalis : an update. (2010)
  • Author(s): Kawada T., Sekiguchi T., Sakai T., Aoyama M., Satake H.
  • Journal Title: Zoolog Sci. 27 Pages: 134-153
  • NAID: 40017358565
  • Peer Reviewed
• [Journal Article] Neuropeptides, hormon Peptides, and Their Receptors in Ciona intestinelis : An Update (2010)
  • Author(s): Tsuyoshi Kawada
  • Journal Title: Zoological Science 27 Pages: 134-153
  • Peer Reviewed
• [Journal Article] A Novel Inhibitory Gonadotropin-releasing Hormone-Related Neuropeptide in the Ascidian, Ciona intestinalis (2009)
  • Author(s): Kawada T., Aoyama M., Okada I., Sakai T., Sekiguchi T., Ogasawara M., Satake H.
  • Journal Title: Peptides 30 Pages: 2200-2205
  • Peer Reviewed
• [Journal Article] Calcitonin in a Protochordate, Ciona intestinalis--the Prototype of the Vertebrate Calcitonin/Calcitonin Gene-related Peptide Superfamily (2009)
  • Author(s): Sekiguchi T., Suzuki N., Fujiwara N., Aoyama M., Kawada T., Sugase K., Murata Y., Sasayama Y., Ogasawara M., Satake H.
  • Journal Title: FEBS J. 276 Pages: 4437-4447
  • Peer Reviewed
• [Presentation] カタユウレイボヤのオキシトシン・バソプレシン様ペプチドの同定および遺伝子発現解析 (2010)
  • Author(s): 川田剛士
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-09-23
• [Presentation] カタユウレイボヤのオキシトシン・バソプレシン様ペプチドの同定および遺伝子発現解析 (2010)
  • Author(s): 川田剛士
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京
• [Presentation] カタユウレイボヤ脳神経節に実在する神経ペプチドの網羅的解析 (2009)
  • Author(s): 川田剛士
  • Organizer: 第34回日本比較内分泌学会大会・日本比較生理生化学会第31回大会合同大会
  • Place of Presentation: 千里ライフサィエンスセンター(大阪府)
  • Year and Date: 2009-10-23
• [Presentation] Characterization of a novel oxytocin/vasopressin superfamily peptide and its receptor from an ascidian, Ciona intestinalis (2009)
  • Author(s): 川田剛士
  • Organizer: 16^<th> International Congress of Comparative Endocrinology
  • Place of Presentation: 香港大学(中国)
  • Year and Date: 2009-06-25
• [Presentation] カタユウレイボヤ脳神経節に実在するペプチドの神経ペプチドの網羅的解析 (2009)
  • Author(s): 川田剛士
  • Organizer: 第34回日本比較内分泌学会大会・日本比較生理生化学会第31回大会合同大会
  • Place of Presentation: 大阪
• [Presentation] カタユウレイボヤのニューロテンシン様ペプチドの機能解析 (2009)
  • Author(s): 川田剛士
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡
• [Presentation] Characterization of a novel oxytocin/vasopressin superfamily peptide and its receptor from an ascidian, Ciona intestinalis (2009)
  • Author(s): Kawada T.
  • Organizer: 16th International Congress of Comparative Endocrinology
  • Place of Presentation: Hong Kong, China
• [Book] Nova Science Publishers, Inc., NY, USA.(eds. Hugo Jastrow and Deniela Feuerbach)"Evolutionary Aspects of Molecular Forms and Biological Functions of Oxytocin Family Peptides" In Handbook of Oxytocin Research : Synthesis, Storage and Release, Actions, and Drug Forms. (2009)
  • Author(s): Kawada T., Sekiguchi T., Sugase K., Kanda A., Satake H.
• [Book] Handbook of Oxytocin Research : Synthesis, Storage and Release, Actions, and Drug Forms (2009)
  • Author(s): Tsuyoshi Kawada
  • Total Pages: 28
  • Publisher: Nova Science Publishers, Inc.(NY, USA)
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