Molecular and morphological evolution on free-living nematodes

• Project/Area Number: 21770094
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biodiversity/Systematics
• Research Institution: Forestry and Forest Products Research Institute
• Principal Investigator: KANZAKI Natsumi 独立行政法人森林総合研究所, 森林微生物研究領域, 主任研究員 (70435585)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2009: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 進化 / 自由生活性線虫 / 昆虫寄生線虫 / 口腔形態 / 形態進化 / 分子進化 / イチジクコバチ / Parasitodiplogaster / Diplogastridae

Research Abstract

Diplogastrid nematodes were collected from Ficus syconia and insects(mostly beetles and wasps) from tropical and subtropical regions in Middle America, East Asia, South-East Asia and Africa. Based upon the morphological observations and molecular phylogenetic analyses, several new species descriptions and redescriptions were conducted. Further, the morphological characters were compared with molecular phylogenetic relationship, and the evolutionary history of stomatal morphologies was examined for diplogastrid nematodes.

Research Products

• [Journal Article] Teratodiplogaster martini n. sp. and Parasitodiplogaster doliostoma n. sp.(Nematoda : Diplogastridae) from the syconia of Ficus species from Africa (2012)
  • Author(s): Kanzaki, N., Giblin-Davis, R. M., Davies, K. A. & Center, B. J
  • Journal Title: Nematology Volume: 14(印刷中掲載確定)
  • Peer Reviewed
• [Journal Article] Description of three nematode species from Japan, Pristionchus exspectatus n. sp., P. arcanus n. sp., and P. japonicus n. sp., which form a cryptic species complex including the model organism P. pacificus(Diplogastridae) (2012)
  • Author(s): Kanzaki, N., Ragsdale, E. J., Herrmann, M., Mayer, W. E. & Sommer, R. J
  • Journal Title: Zoological Science Volume: 29(印刷中掲載確定)
  • Peer Reviewed
• [Journal Article] Parapristionchus giblindavisi n. gen, n. sp.(Rhabditida : Diplogastridae) isolated from stag beetles(Coleoptera : Lucanidae) in Japan (2012)
  • Author(s): Kanzaki, N., Ragsdale, E. J., Herrmann, M., Mayer, W. E., Tanaka, R. & Sommer, R. J
  • Journal Title: Nematology Volume: 14(印刷中掲載確定)
  • Peer Reviewed
• [Journal Article] Two new species of Pristionchus(Rhabditida : Diplogastridae): P. fissidentatus n. sp. from Nepal and La Reunion Island and P. elegans n. sp. from Japan (2012)
  • Author(s): Kanzaki, N., Ragsdale, E. J., Herrmann, M. & Sommer, R. J
  • Journal Title: Journal of Nematology Volume: 44(印刷中掲載確定)
  • Peer Reviewed
• [Journal Article] Teratodiplogaster martini n.sp.and Parasitodiplogaster doliumstoma n.sp.(Nematoda : Diplogastridae) from the syconia of Ficus species from Africa (2012)
  • Author(s): Kanzaki, N., Giblin-Davis, R.M., Davies, K.A., Center, B.J.
  • Journal Title: Nematology Volume: 14(In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Description of three nematode species from Japan, Pristionchus exspectatus n.sp., p.arcanus n.sp., and P.japonicus n.sp., which form a cryptic species complex including the model organism P.pacificus (Diplogastridae) (2012)
  • Author(s): Kanzaki, N., Ragsdale, E.J., Herrmann, M., Mayer, W.E., Sommer, R.J.
  • Journal Title: Zoological Science Volume: 29(In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Parapristionchus giblindavisi n.gen, n.sp.(Rhabditida : Diplogastridae) isolated from stag beetles (Coleoptera : Lucanidae) in Japan (2012)
  • Author(s): Kanzaki, N., Ragsdale, E.J., Herrmann, M., Mayer, W.E., Tanaka, R., Sommer, R.J.
  • Journal Title: Nematology Volume: 14(In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Two new species of Pristionchus (Rhabditida : Diplogastridae) : P.fissidentatus n.sp.from Nepal and La Reunion Island and P.elegans n.sp.from Japan (2012)
  • Author(s): Kanzaki, N., Ragsdale, E.J., Herrmann, M., Sommer, R.J.
  • Journal Title: Journal of Nematology Volume: 44(In press)(掲載確定)
  • Peer Reviewed
• [Journal Article] Poikilolaimus carsiops n. sp.(Rhabditida : Rhabditidae) associated with Neotermes koshunensis(Kalotermitidae) in Kenting National Park, Taiwan (2011)
  • Author(s): Kanzaki, N., Li, H.-F., Lan, Y.-C., Kosaka, H., Giblin-Davis, R. M. & Center, B. J
  • Journal Title: Nematology Volume: 13 Pages: 155-164
  • Peer Reviewed
• [Journal Article] Poikilolaimus carsiops n.sp.(Rhabditida : Rhabditidae) associated with Neotermes koshunensis (Kalotermitidae) in Kenting National Park, Taiwan (2011)
  • Author(s): Kanzaki, N., Li, H.-F., Lan, Y.-C., Kosaka, H., Giblin-Davis, R.M., Center, B.J.
  • Journal Title: Nematology Volume: 13 Pages: 155-164
  • Peer Reviewed
• [Journal Article] Redescription of two Panamanian nematodes, Parasitodiplogaster citrinema Poinar & Herre, 1991 and P. popenema Poinar & Herre, 1991 (2010)
  • Author(s): Kanzaki, N., Giblin-Davis, R. M., Herre, E. A. & Center, B. J
  • Journal Title: Nematology Volume: 12 Pages: 89-104
  • Peer Reviewed
• [Journal Article] Acrostichus megaloptae n. sp.(Nematoda : Diplogastridae), a phoretic associate of Megalopta spp.(Hymenoptera : Halictidae) in Central America (2010)
  • Author(s): Kanzaki, N., Giblin-Davis, R. M., Wcislo, W. T., Zeng, Y., Ye, W., Center, B. J., Esquivel, A. & Thomas, W. K
  • Journal Title: Nematology Volume: 12 Pages: 453-468
  • Peer Reviewed
• [Journal Article] Ultrastructure and life history of Myolaimus byersi n. sp.; a phoretic associate of the crane fly Limonia schwarzi(Alexander)(Tipulidae) in Florida (2010)
  • Author(s): Giblin-Davis, R. M., Kanzkai, N., Williams, D. S., De Ley, P., Schreirenberg, E., Zeng, Y. Center, B. J
  • Journal Title: Nematology Volume: 12 Pages: 519-542
  • Peer Reviewed
• [Journal Article] Pseudaphelenchus vindai n. sp.(Tylenchomorpha : Aphelenchoididae) associated with termites(Termitidae) in Barro Colorado Island, Panama (2010)
  • Author(s): Kanzaki, N., Giblin-Davis, R. M., Herre, E. A, Scheffrahn, R. H. & Center, B. J
  • Journal Title: Nematology Volume: 12 Pages: 905-914
  • Peer Reviewed
• [Journal Article] Ditylenchus halictus n. sp.(Nematoda : Anguinidae), an associate of the sweat bee Halictus sexcinctus(Halictidae) from Germany (2010)
  • Author(s): Giblin-Davis, R. M., Erteld, C., Kanzaki, N., Ye, W., Zeng, Y., & Center, B. J
  • Journal Title: Nematology Volume: 12 Pages: 891-904
  • Peer Reviewed
• [Journal Article] Schistonchus(Aphelenchoididae) from Ficus(Moraceae) in Australia, with description of S. aculeata sp. n (2010)
  • Author(s): Davies, K. A., Bartholomaeus, F., Ye, W., Kanzaki, N. & Giblin-Davis, R. M
  • Journal Title: Nematology Volume: 12 Pages: 935-958
  • Peer Reviewed
• [Journal Article] Acrostichus megaloptae n.sp.(Nematoda : Diplogastridae), a phoretic as sociate of Megalopta spp.(Hymenoptera : Halictidae) in Central America. (2010)
  • Author(s): Kanzaki, N., Giblin-Davis, R.M., Wcislo, W.T., Zeng, Y., Ye, W., Center, B.J., Esquivel, A., Thomas, W.K.
  • Journal Title: Nematology Volume: 12 Pages: 453-468
  • Peer Reviewed
• [Journal Article] Pseudaphelenchus vindai n.sp.(Tylenchomorpha : Aphelenchoididae) associated with termites (Termitidae) in Barro Colorado Island, Panama. (2010)
  • Author(s): Kanzaki, N., Giblin-Davis, R.M., Herre, E.A., Scheffrahn, R.H., Center, B.J.
  • Journal Title: Nematology Volume: 12 Pages: 905-914
  • Peer Reviewed
• [Journal Article] Schistonchus (Aphelenchoididae) from Ficus (Moraceae) in Australia, with description of S.aculeata sp.n. (2010)
  • Author(s): Davies, K., Bartholomaeus, F., Ye, W., Kanzaki, N., Giblin-Davis, R.M.
  • Journal Title: Nematology Volume: 12 Pages: 935-958
  • Peer Reviewed
• [Journal Article] Redescription of two Panamanian nematodes, Parasitodiplogaster citrinema Poinar & Herre, 1991 and P.popenema Poinar & Herre, 1991 (2010)
  • Author(s): Kanzaki, N., Giblin-Davis, R.M., Herre, E.A., Center, BJ.
  • Journal Title: Nematology 12 Pages: 89-104
  • Peer Reviewed
• [Journal Article] Schistonchus virens sp. n.(Aphelenchoididae) and Parasitodiplogaster australis sp. n.(Diplogastridae), from Ficus virens(Moraceae) in Australia (2009)
  • Author(s): Bartholomaeus, F., Davies K. A., Ye, W., Kanzaki, N., & Giblin-Davis, R. M
  • Journal Title: Nematology Volume: 11 Pages: 583-601
  • Peer Reviewed
• [Journal Article] Acrostichus rhynchophori n. sp.(Rhabditida : Diplogastridae): a phoretic associate of Rhynchophorus cruentatus Fabricius and R. palmarum L.(Coleoptera : Curculionidae) in the America (2009)
  • Author(s): Kanzaki, N., Giblin-Davis, R. M., Zeng, Y., Ye, W. & Center, B. J
  • Journal Title: Nematology Volume: 11 Pages: 669-688
  • Peer Reviewed
• [Journal Article] Tropical nematode diversity : vertical stratification of nematode communities in a Costa Rican humid lowland rainforest (2009)
  • Author(s): Powers. T. O., Neher, D. A., Mullin, P., Esquivel, A., Giblin-Davis, R. M., Kanzaki, N., Stock, S. P., Mora, M. M. & Uribe-Lorio, L
  • Journal Title: Molecular Ecology Volume: 18 Pages: 985-996
  • Peer Reviewed
• [Journal Article] Evaluating high throughput sequencing as a method for metagenomic analysis of nematode diversity (2009)
  • Author(s): Porazinska, D. L., Giblin-Davis, R. M., Farmerie, W., Powers, T. O., Kanzaki, N., Faller, L., Morris, K., Sung, W. & Thomas, W. K
  • Journal Title: Molecular Ecology Resources Volume: 9 Pages: 1439-1450
  • Peer Reviewed
• [Journal Article] Teratodiplogaster fignewmani gen. nov., sp. nov.(Nematoda : Diplogastridae) from the syconia of Ficus racemosa in Australia (2009)
  • Author(s): Kanzaki, N., Giblin-Davis, R. M., Davies, K., Ye, W., Center, B. J. & Thomas, W. K
  • Journal Title: Zoological Science Volume: 26 Pages: 569-578
  • NAID: 40016772433
  • Peer Reviewed
• [Journal Article] Dauer juveniles of Poikilolaimus floridensis.(Rhabditida : Rhabditidae) isolated from drywood termites(Kalotermitidae) (2009)
  • Author(s): Kanzaki, N., Giblin-Davis, R. M., Scheffrahn, R. H. & Center, B. J
  • Journal Title: Nematropica Volume: 39 Pages: 287-292
  • Peer Reviewed
• [Journal Article] Pseudaphelenchus yukiae n. gen., n. sp.(Tylenchina : Aphelenchidae) associated with Cylindrotermes macrognathus(Termitidae : Termitinae) in La Selva, Costa Rica (2009)
  • Author(s): Kanzaki, N., Giblin-Davis, R. M., Scheffrahn, R. H., Center, B. J. & Davies, K. A
  • Journal Title: Nematology Volume: 11 Pages: 869-881
  • Peer Reviewed
• [Journal Article] Acrostichus puri n. sp.(Nematoda : Diplogastridae), a phoretic associate of Augochlora pura mosieri Cockerell(Hymenoptera : Halictidae) (2009)
  • Author(s): Kanzaki, N., Giblin-Davis, R. M., Zeng, Y., Ye, W., Center, B. J. & Thomas, W. K
  • Journal Title: Nematology Volume: 12 Pages: 49-64
  • Peer Reviewed
• [Journal Article] Teratodiplogaster fignewmani gen.nov., sp.nov.(Nematoda : Diplogastridae)from the syconia of Ficus racemosa in Australia (2009)
  • Author(s): Kanzaki, N., Giblin-Davis, R.M., Davies, K., Ye, W., Center, B.J. Thomas, W.K.
  • Journal Title: Zoological Science 26 Pages: 569-578
  • Peer Reviewed
• [Journal Article] Schistonchus virens sp.n.(Aphelenchoididae)and Parasitodiplogaster australis sp.n.(Diplogastridae), from Ficus virens(Moraceae)in Australia (2009)
  • Author(s): Bartholomaeus, F., Davies K.A., Ye, W., Kanzaki, N., Giblin-Davis, R.M.
  • Journal Title: Nematology 11 Pages: 583-601
  • Peer Reviewed
• [Presentation] Reverse taxonomy for elucidating diversity of termite-associated nematodes (2011)
  • Author(s): Kanzaki, N., Giblin-Davis, R. M., Scheffrahn, R. H., Taki, H., Esquivel, A., Davies, K. A. & Herre, E. A
  • Organizer: The 50th Annual Meeting of Society of Nematologists
  • Place of Presentation: Oregon State University(Corvallis, Oregon, USA)
  • Year and Date: 2011-07-20
• [Presentation] The amazing fig-nematodes of Martin et al.: forty years on (2011)
  • Author(s): Giblin-Davis, R. M., Kanzaki, N., Davies, K. & Center, B. J
  • Organizer: The 50th Annual Meeting of Society of Nematologists
  • Place of Presentation: Oregon State University(Corvallis, Oregon, USA)
  • Year and Date: 2011-07-20
• [Presentation] First report of pinworms from termite hindguts from central America. (2010)
  • Author(s): Giblin-Davis, R.M., Kanzaki, N., Esquivel, A., Davies, K.A., Herre, E.A., Center, B.J., Scheffrahn, R.H.
  • Organizer: The 49th Annual Meeting of Society of Nematologists
  • Place of Presentation: アイダホ大学(アメリカ・アイダホ)
• [Presentation] Termite-associated nematodes in Kenting National Park, Taiwan and Miyako and Hateruma islands, Okinawa, Japan. (2010)
  • Author(s): Kanzaki, N., Li, H.-F., Lan, T.-C., Kosaka, H., Gibin-Davis, R.M., Akiba, M., Sahashi, N., Ota, Y.
  • Organizer: XXIII IUFRO World Congress
  • Place of Presentation: COEX Center(韓国・ソウル)
• [Presentation] Termite associated nematodes : their association pattern and evolutional relationship. (2010)
  • Author(s): Kanzaki, N.
  • Organizer: Symposium and Workshop of Termite Biology and Control in Taiwan
  • Place of Presentation: 台湾国立大学(台湾・台北)(招待講演)
• [Presentation] Termite-associated nematodes in Kenting national park (2009)
  • Author(s): Kanzaki, N., Li, H.-F., Lan, Y.-C., Kosaka, H. & Giblin-Davis, R. M
  • Organizer: The 48th Annual Meeting of Society of Nematologists
  • Place of Presentation: University of Vermont(Burlington, Vermont, USA)
  • Year and Date: 2009-08-18
• [Presentation] Morphological observation of two Panamanian nematodes, Parasitodiplogaster citrinema Poinar & Herre, 1991 and P. popenema Poinar & Herre, 1991 with a comparison with molecular phylogenetic relationship (2009)
  • Author(s): Kanzaki, N., Giblin-Davis, R. M., Herre, E. A. & Center, B. J
  • Organizer: The 48th Annual Meeting of Society of Nematologists
  • Place of Presentation: University of Vermont(Burlington, Vermont, USA)
  • Year and Date: 2009-08-18
• [Presentation] Nematode associations with wild bees from global diversity survey (2009)
  • Author(s): Giblin-Davis, R. M., Kanzaki, N., Hazir, C., Hazir, S., Wcislo, W., Ye, W. & Thomas, W. K
  • Organizer: The 48th Annual Meeting of Society of Nematologists
  • Place of Presentation: University of Vermont(Burlington, Vermont, USA)
  • Year and Date: 2009-08-18
• [Presentation] Morphological observation of two Panamanian nematodes, Parasitodiplogaster citrinema Poinar & Herre, 1991 and P.popenema Poinar & Herre, 1991 with a comparison with molecular phylogenetic relationship (2009)
  • Author(s): Kanzaki, N., Giblin-Davis, R.M., Herre, E.A. Center, B.J.
  • Organizer: 第48回アメリカ線虫学会大会
  • Place of Presentation: Burlington, Vermont, USA