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Structural and functional analysis of Uro-adherence factor A(UafA) from Staphylococcus saprophyticus

Research Project

Project/Area Number 21770101
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionHokkaido University

Principal Investigator

TANAKA Yoshikazu  北海道大学, 創成研究機構, 特任助教 (20374225)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywords接着因子 / 結晶構造解析 / 腐性ブドウ球菌
Research Abstract

Pathogenic bacteria express several cell wall-anchored proteins to adhere to the surface of host cell, whereas Staphylococcus saprophyticus, a uropathogen involved in urinary tract infection of young females, possesses a unique cell wall-anchored protein, UafA. In this study, we investigated the adhesion mechanism of UafA by structure analysis and biochemical assay.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (31 results)

All 2012 2011 2010 2009 Other

All Journal Article (14 results) (of which Peer Reviewed: 14 results) Presentation (16 results) Remarks (1 results)

  • [Journal Article] Crystal Structure of the Octameric Pore of Staphylococcalγ-hemolysin Reveals theβ-barrel Pore Formation Mechanism by Two Components2011

    • Author(s)
      Keitaro Yamashita, Yuka Kawai, Yoshikazu Tanaka, Nagisa Hirano, Jun Kaneko, Noriko Tomita, Makoto Ohta, Yoshiyuki Kamio, Min Yao, and Isao Tanaka
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 108 Pages: 17314-17319

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular basis of dihydrouridine formation on tRNA2011

    • Author(s)
      Futao Yu, Yoshikazu Tanaka, Keitaro Yamashita, Takeo Suzuki, Akiyoshi Nakamura, Nagisa Hirano, Tsutomu Suzuki, Min Yao, and Isao Tanaka
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 108 Pages: 19593-19598

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the functional region of Uro-adherence factor A(UafA) from Staphylococcus saprophyticus reveals participation of the B domain in ligand binding2011

    • Author(s)
      Eriko Matsuoka, Yoshikazu Tanaka, Makoto Kuroda, Yuko Shouji, Toshiko Ohta, Isao Tanaka, and Min Yao
    • Journal Title

      Protein Science

      Volume: 20 Pages: 406-416

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Structural and Mutational Analyses of the Receptor Binding Domain of Botulinum D/C Mosaic Neurotoxin : Insight into the Ganglioside Binding Mechanism2011

    • Author(s)
      Nuemket Nipawan, Yoshikazu Tanaka, Kentaro Tsukamoto, Takao Tsuji, Keiji Nakamura, Shunji Kozaki, Min Yao, and Isao Tanaka
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 411 Pages: 433-439

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystallization and preliminary X-ray crystallographic analysis of dihydrouridine synthase from Thermus thermophilus and its complex with tRNA2011

    • Author(s)
      Futao Yu, Yoshikazu Tanaka, Shiho Yamamoto, Akiyoshi Nakamura, Shunsuke Kita, Nagisa Hirano, Isao Tanaka, and Min Yao
    • Journal Title

      Acta Crystallographica Section F

      Volume: F67 Pages: 685-688

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 2-Methyl-2, 4-pentanediol induces spontaneous assembly of staphylococcal alpha-hemolysin into heptameric pore structure2011

    • Author(s)
      Yoshikazu Tanaka, Nagisa Hirano, Jun Kaneko, Yoshiyuki Kamio, Min Yao, and Isao Tanaka
    • Journal Title

      Potein Science

      Volume: 20 Pages: 448-456

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the octameric pore of staphylococcal γ-hemolysin reveals the β-barrel pore formation mechanism by two components2011

    • Author(s)
      Keitaro Yamashita, Yuka Kawai, Yoshikazu Tanaka, Nagisa Hirano, Jun Kaneko, Noriko Tomita, Makoto Ohta, Yoshiyuki Kamio, Min Yao, Isao Tanaka
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 108 Issue: 42 Pages: 17314-17319

    • DOI

      10.1073/pnas.1110402108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural and Mutational Analyses of the Receptor Binding Domain of Botulinum D/C Mosaic Neurotoxin : Insight into the Ganglioside Binding Mechanism2011

    • Author(s)
      Nuemket Nipawan, Yoshikazu Tanaka, Kentaro Tsukamoto, Takao Tsuji, Keiji Nakamura, Shunji Kozaki, Min Yao, Isao Tanaka
    • Journal Title

      Biochemical and Biophysical Research Communication

      Volume: 411 Pages: 433-439

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Crystal structure of the functional region of Uro-adherence factor A (UafA) from Staphylococcus saprophyticus reveals participation of the B do main in ligand binding.2011

    • Author(s)
      E.Matsuoka, Y.Tanaka, M.Kuroda, Y.Shouji, T.Ohta, I.Tanaka, M.Yao
    • Journal Title

      Protein Science

      Volume: 20 Pages: 406-416

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 2-Methyl-2,4-pentanediol induces spontaneous assembly of staphylococcal alpha-hemolysin into heptameric pore structure2011

    • Author(s)
      Y.Tanaka, N.Hirano, J.Kaneko, Y.Kamio, M.Yao, I.Tanaka
    • Journal Title

      Protein Science

      Volume: 20 Pages: 448-456

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Structural and Energetic Hot-Spots for the Interaction between a Ladder-like Polycyclic Ether and the Anti-Ciguatoxin Antibody 10C9Fab.2011

    • Author(s)
      M.Ui, Y.Tanaka, T.Tsumuraya, I.Fujii, M.Inoue, M.Hirama, K.Tsumoto
    • Journal Title

      Molecular BioSystems

      Volume: 7 Pages: 793-798

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Deduced RNA binding mechanism of ThiI based on structural and binding analyses of a minimal RNA ligand2009

    • Author(s)
      Y.Tanaka, S.Yamagata, Y.Kitago, Y.Yamada, S.Chimnaronk, M.Yao, I.Tanaka
    • Journal Title

      RNA 15

      Pages: 1498-1506

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The interaction of hyperthermophilic TATA-box binding protein with single-stranded DNA is entropically favorable and exhibits a large negative heat capacity change at high salt conce ntration2009

    • Author(s)
      S.Nagatoishi, Y.Tanaka, M.Kudou, K.Tsumoto
    • Journal Title

      Mol.BioSyst. 5

      Pages: 957-961

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Contributions of interfacial residues of human Interleukin15 to the specificity and affinity for its private alpha-receptor2009

    • Author(s)
      S.Sakamoto, J.M.Caaveiro, E.Sano, Y.Tanaka, M.Kudou, K.Tsumoto
    • Journal Title

      J.Mol.Biol. 389

      Pages: 880-894

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] Crystal structure of pore-forming toxin from Staphylococcus aureus-An example of the utility of crystal structure analysis-2012

    • Author(s)
      Yoshikazu Tanaka, Keitaro Yamashita, Yuka Kawai, Jun Kaneko, Noriko Tomita, Makato Ohta, Yoshiyuki Kamio, Min Yao, and Isao Tanaka
    • Organizer
      Japan-Australia Symposium on Plant Sciences for Agriculture(JAS2012)
    • Place of Presentation
      北海道大学(札幌市)
    • Year and Date
      2012-01-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Crystal structure of pore-forming toxin from Staphylococcus aureus-An example of the utility of crystal structure analysis-2012

    • Author(s)
      Yoshikazu Tanaka, Keitaro Yamashita, Yuka Kawai, Jun Kaneko, Noriko Tomita, Makato Ohta, Yoshiyuki Kamio, Min Yao, Isao Tanaka
    • Organizer
      Japan-Australia Symposium on Plant Sciences for Agriculture (JAS2012)
    • Place of Presentation
      北海道
    • Year and Date
      2012-01-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] Thermus thermophilus由来ジヒドロウリジン合成酵素とtRNA複合体の結晶構造解析2011

    • Author(s)
      田中良和, Yu Futao, 山下恵太郎, 鈴木健夫, 中村彰良, 平野なぎ沙, 鈴木勉, 姚閔, 田中勲
    • Organizer
      日本結晶学会年会
    • Place of Presentation
      北海道
    • Year and Date
      2011-11-25
    • Related Report
      2011 Annual Research Report
  • [Presentation] Crystal Structure of the Octameric Pore of Staphylococcal gamma-hemolysin2011

    • Author(s)
      Yoshikazu Tanaka, Keitaro Yamashita, Yuka Kawai, Nagisa Hirano, Jun Kaneko, Noriko Tomita, Makato Ohta, Yoshiyuki Kamio, Min Yao, and Isao Tanaka
    • Organizer
      The Eighth International Conference on Flow Dynamics(ICFD2011)
    • Place of Presentation
      ホテルメトロポリタン仙台(仙台市)
    • Year and Date
      2011-11-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Crystal Structure of the Octameric Pore of Staphylococcal gamma-hemolysin2011

    • Author(s)
      Yoshikazu Tanaka, Keitaro Yamashita, Yuka Kawai, Nagisa Hirano, Jun Kaneko, Noriko Tomita, Makato Ohta, Yoshiyuki Kamio, Min Yao, Isao Tanaka
    • Organizer
      The Eighth International Conference on Flow Dynamics (ICFD2011)
    • Place of Presentation
      宮城県
    • Year and Date
      2011-11-10
    • Related Report
      2011 Annual Research Report
  • [Presentation] 黄色ブドウ球菌由来巨大蛋白質Ebhの全体構造に関する研究2011

    • Author(s)
      田中良和
    • Organizer
      日本化学会東北支部化学系学協会東北大会
    • Place of Presentation
      東北大学(仙台市)
    • Year and Date
      2011-09-17
    • Related Report
      2011 Final Research Report
  • [Presentation] 黄色ブドウ球菌由来巨大蛋白質Ebhの全体構造に関する研究2011

    • Author(s)
      田中良和
    • Organizer
      日本化学会東北支部化学系学協会東北大会
    • Place of Presentation
      宮城県
    • Year and Date
      2011-09-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] Crystal structure of dihydrouridine synthase in complex with RNA reveals its tRNA recognition and reaction mechanisms2011

    • Author(s)
      Yoshikazu Tanaka, Futao Yu, Keitaro Yamashita, Akiyoshi Nakamura, Takeo Suzuki, Tsutomu Suzuki, Min Yao, and Isao Tanaka
    • Organizer
      The 16th Annual Meeting of the RNA Society(Kyoto)
    • Place of Presentation
      国立京都国際会館(京都府)
    • Year and Date
      2011-06-17
    • Related Report
      2011 Final Research Report
  • [Presentation] Crystal structure of dihydrouridine synthase in complex with RNA reveals its tRNA recognition and reaction mechanisms2011

    • Author(s)
      Yoshikazu Tanaka, Futao Yu, Keitaro Yamashita, Akiyoshi Nakamura, Takeo Suzuki, Tsutorou Suzuki, Min Yao, Isao Tanaka
    • Organizer
      The 16th Annual Meeting of the RNA Society (Kyoto)
    • Place of Presentation
      京都府
    • Year and Date
      2011-06-17
    • Related Report
      2011 Annual Research Report
  • [Presentation] RNA binding mechanism of ThiI deduced from structural and binding analyses of a minimal RNA ligand2010

    • Author(s)
      Yoshikazu Tanaka, Shiori Yamagata, Yu Kitago, Yoko Yamada, Sarin Chimnaronk, Min Yao and Isao Tanaka
    • Organizer
      Asian Crystallographic Association
    • Place of Presentation
      Busan, Korea
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] 部分RNAの結晶構造解析から推察されたtRNAチオ化修飾酵素ThiIのtRNA認識機構2010

    • Author(s)
      田中良和
    • Organizer
      九州シンポジウム
    • Place of Presentation
      玄海ロイヤルホテル(宗像市)
    • Related Report
      2011 Final Research Report 2010 Annual Research Report
  • [Presentation] Deduced RNA binding mechanism of ThiI2010

    • Author(s)
      Yoshikazu Tanaka, Shiori Yamagata, Yu Kitago, Yoko Yamada, Sarin Chimnaronk, Min Yao, Isao Tanaka
    • Organizer
      Protein Society
    • Place of Presentation
      San Diego, USA
    • Related Report
      2010 Annual Research Report
  • [Presentation] 黄色ブドウ球菌由来α-hemolysinの自発的7量体形成2010

    • Author(s)
      田中良和, 平野なぎ沙, 河内宏樹, 山下恵太郎, 金子淳, 神尾好是, 姚閔, 田中勳
    • Organizer
      蛋白質科学会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] 黄色ブドウ球菌由来a-ヘモリジンは自発的に7量体へと会合する2010

    • Author(s)
      田中良和, 平野なぎ沙, 河内宏樹, 山下恵太郎, 金子淳, 神尾好是, 姚閔, 田中勳
    • Organizer
      毒素シンポジウム
    • Place of Presentation
      長浜ロイヤルホテル(長浜市)
    • Related Report
      2010 Annual Research Report
  • [Presentation] tRNAチオ化修飾酵素ThiIのRNA認識機構の解析2009

    • Author(s)
      田中良和, 山形しおり, 北郷悠, 山田洋子, チムナロンサリン, 姚閔, 田中勲
    • Organizer
      RNA学会
    • Place of Presentation
      新潟、新潟コンベンミョンセンター
    • Year and Date
      2009-07-27
    • Related Report
      2009 Annual Research Report
  • [Presentation] tRNAチオ化修飾酵素 ThiIのtRNA認識機構2009

    • Author(s)
      田中良和, 山形しおり, 北郷悠, 山田洋子, チムナロンサリン, 姚閔, 田中勲
    • Organizer
      日本蛋白質科学会
    • Place of Presentation
      熊本、熊本全日空ホテルニュースカイ
    • Year and Date
      2009-05-22
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://altair.sci.hokudai.ac.jp/g6/

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2019-06-06  

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