| Project/Area Number |
21770106
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Kyoto Sangyo University (2010)
Tokyo Institute of Technology (2009) |
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Principal Investigator |
SUNO Ryoji Kyoto Sangyo University, 総合生命科学部, 特定研究員(PD) (60447521)
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| Co-Investigator(Renkei-kenkyūsha) |
AKIYAMA Yoshinori 京都大学, ウイルス研究所, 教授 (10192460)
IWATA So 京都大学, 医学研究科, 教授 (60452330)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | X線結晶構造解析 / AAA+タンパク質 / ATP依存性プロテアーゼ / 超分子複合体 / 酵素 / プロテアーゼ / タンパク質 / AAA+ファミリータンパク質 / 酵素反応 / 構造解析 / X線構造解析 / ATP加水分解 / 品質管理 |
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| Research Abstract |
It is the purpose of this research that ATP-dependent substrate degradation mechanism of membrane bound AAA+ protease FtsH is revealed by X-ray crystallographic analysis. During this study period, crystals of the functional cytosolic retion of FtsH were obtained in several new crystallization conditions including a few kinds of ATP analogues. These crystals difftacted to a resolution of about 3.5 Å, and now we are analyzing these data to determine new crystal structures of sFtsH bound several ATP analogues. It is expected that these structures show the detailed molecular mechanism of FtsH.
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