Functional analysis of E. coli Hsp90 (HtpG) to ribosome biogenesis
| Project/Area Number |
21770107
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Tokyo Institute of Technology |
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Principal Investigator |
MOTOJIMA Fumihiro Tokyo Institute of Technology, 総合生命科学部, 助教 (70372464)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 変性とフォールディング / 分子シャペロン / HtpG / Hsp90 / リボソーム / リボソームサブユニット |
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| Research Abstract |
We found that a 50S ribosomal protein L2 was purified as a complex with His-tagged HtpG from Escherichia coli cultured in minimum medium at 45oC. L2 specifically activated ATPase of HtpG more than ten-fold. HtpG was also found in fractions of 30S and 50S ribosome subunits in vivo. These results suggested that HtpG associates with various ribosomal proteins including L2 as a client protein and contributes for ribosome biogenesis.
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Report
(3 results)
Research Products
(4 results)
