The Structural Analysis of Ribonucleaprotein complex vault

• Project/Area Number: 21770111
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: University of Hyogo
• Principal Investigator: KATO Koji University of Hyogo, 大学院・生命理学研究科, 特任助教 (30452428)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: vault / 核酸-タンパク質複合体 / 生体超分子複合体 / X線結晶構造解析 / タンパク質-核酸複合体 / ボルト

Research Abstract

A good quality crystal was obtained by improving the crystallization condition, and it diffracted up to 2.9Å resolution at beam line BL44XU of SPring-8. It is expected that the high resolution structure become an important footholds for clarifying the function of the vault in vivo.

Research Products

• [Journal Article] Structure of Rat Liver Vault at 3.5 A Resolution (2009)
  • Author(s): 加藤公児、田中秀明
  • Journal Title: SPring-8 Research Frontiers Pages: 28-29
  • Peer Reviewed
• [Journal Article] 分子量が1000万にも及ぶ生体巨大粒子に対する構造解析 (2009)
  • Author(s): 山下栄樹、加藤公児、田中秀明
  • Journal Title: 放射光学会誌 Vol.22 Pages: 284-291
  • Peer Reviewed
• [Journal Article] 細胞内にある巨大な核酸-蛋白質複合体vault のX線結晶構造解析 (2009)
  • Author(s): 加藤公児、田中秀明
  • Journal Title: タンパク質、核酸、酵素 Vol.51 Pages: 1159-1165
  • Peer Reviewed
• [Journal Article] ラット肝由来の巨大な核酸タンパク質複合体vaultのX 線結晶構造 (2009)
  • Author(s): 住澤知之、加藤公児、田中秀明
  • Journal Title: 実験医学 Vol.27 Pages: 1751-1754
  • Peer Reviewed
• [Journal Article] 分子量約1000 万の巨大粒子vault のX 線結晶構造解析 (2009)
  • Author(s): 田中秀明、加藤公児、山下栄樹
  • Journal Title: 結晶学会誌 Vol.51 Pages: 189-194
  • NAID: 10025158264
  • Peer Reviewed
• [Journal Article] ラット肝臓由来の巨大な核酸タンパク質複合体vaultのX線結晶構造解析 (2009)
  • Author(s): 住澤知之
  • Journal Title: 実験医学 27 Pages: 1751-1754
  • Peer Reviewed
• [Journal Article] 細胞内にある巨大な核酸-蛋白質複合体vaultのX線結晶構造解析 (2009)
  • Author(s): 加藤公児
  • Journal Title: 蛋白質核酸酵素 51 Pages: 1159-1165
  • Peer Reviewed
• [Journal Article] 分子量1000万の巨大粒子vaultのX線結晶構造解析 (2009)
  • Author(s): 田中秀明
  • Journal Title: 結晶学会誌 51 Pages: 189-194
  • Peer Reviewed
• [Journal Article] 分子量が1000万にも及ぶ生体巨大粒子に対する構造解析 (2009)
  • Author(s): 山下栄樹
  • Journal Title: 放射光学会誌 22 Pages: 284-291
  • Peer Reviewed
• [Presentation] The structure of rat liver vault at 3.5 resolution (2010)
  • Author(s): 加藤公児
  • Organizer: 第10回アジア結晶学会
  • Place of Presentation: プサン、韓国
  • Year and Date: 2010-11-01
• [Presentation] The structure of rat liver vault at 3.5 A resolution (2010)
  • Author(s): 加藤公児
  • Organizer: 第10回アジア結晶学会
  • Place of Presentation: プサン(韓国)
  • Year and Date: 2010-11-01
• [Presentation] 巨大な超分子複合体を見る (2010)
  • Author(s): 加藤公児
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
  • Year and Date: 2010-06-18
• [Presentation] 巨大な超分子を見る (2010)
  • Author(s): 加藤公児
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 北海道
  • Year and Date: 2010-06-16
• [Presentation] The structure of rat liver vault at 3.5 resolution (2009)
  • Author(s): 加藤公児
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島県
  • Year and Date: 2009-10-30
• [Presentation] The structure of rat liver vault at 3.5Å resolution (2009)
  • Author(s): 加藤公児
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島県
  • Year and Date: 2009-10-30
• [Presentation] The structure of rat liver vault at 3.5 A resolution (2009)
  • Author(s): 加藤公児
  • Organizer: The 3^<rd> International Congress of Nanobiotechnology and Nanomedicine
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2009-06-22
• [Presentation] The structure of rat liver vault at 3.5Å resolution (2009)
  • Author(s): 加藤公児
  • Organizer: The 3^<rd> International Congress of Nanobiotechnology and Nanomedicine
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2009-06-22
• [Remarks] ホームページ等
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• [Remarks]
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• [Remarks]
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