Structural basis of the complex proteins involved in the iron-sulfur cluster biogenesis
Project/Area Number |
21770112
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
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Research Institution | Osaka University |
Principal Investigator |
WADA Kei Osaka University, 大学院・理学研究科, 助教 (80379304)
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Project Period (FY) |
2009 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
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Keywords | 鉄硫黄クラスター / X線結晶構造解析 / ATP分解酵素 / SUFマシナリー / ISCマシナリー |
Research Abstract |
In this study, the quaternary structure of the SufC_2-SufD_2 complex involved in Fe-S cluster biogenesis in which SufC is bound to the C-terminal domain of SufD was determined. Comparison with the monomeric structure of SufC revealed conformational change of the active-site residues : SufC becomes competent for ATP binding and hydrolysis upon association with SufD. These findings, together with biochemical characterization of the SufB-SufC-SufD complex, have led us to propose a model for the Fe-S cluster biosynthesis in the complex.
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Report
(3 results)
Research Products
(28 results)
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[Journal Article] Expression, purification and preliminary X-ray crystallographic analysis of the cyanobacterial biliverdin reductase2011
Author(s)
Watanabe, A., Hirata, K., Hagiwara, Y., Yutani, Y., Sugishima, M., Yamamoto, M., Fukuyama, K., Wada K.
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Journal Title
Acta Crystallograph.Sect.F Struct.Biol.Cryst.Commun.
Volume: F67
Pages: 313-317
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Peer Reviewed
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[Journal Article] Molecular Dynamism of Fe-S Cluster Biosynthesis Implicated by the Structure of the SufC_2-SufD_2 Complex.2009
Author(s)
Wada, K., Sumi, N., Nagai, R., Iwasaki, K., Sato, T., Suzuki, K., Hasegawa, Y., Kitaoka, S., Minami, Y., Outten, W., F., Takahashi, Y., Fukuyama, K
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Journal Title
K.J.Mol.Biol. 387
Pages: 245-258
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Peer Reviewed
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[Presentation] Crystal Structure of the SufC2-SufD2 Complex : Implications for Dynamic Rearrangement During Fe-S cluster Biosynthesis.2009
Author(s)
Wada, K., Sumi, N., Nagai, R., Iwasaki, K., Sato, T. Suzuki, K., Nagasaka, Y., Minami., Y., Outten, F.W., Takahashi, Y., Fukuyama., K.
Organizer
Iron-Sulfur Cluster Biogenesis and Regulation Conference
Place of Presentation
Geogia, USA
Year and Date
2009-09-01
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