Structural study of the cell adhesion regulated by tension

• Project/Area Number: 21770114
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: HIRANO Yoshinori 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50452529)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 細胞接着 / メカノバイオロジー / 張力センサー / カテニン / 分子複合体 / 結晶構造解析 / 張力 / 力学センサー / X線結晶構造解析

Research Abstract

Structural studies of ・-Catenin especially focused on its novel function as a mechanosensor are promoted. As the result, we succeeded in visualizing the tension-dependent interaction between ・-Catenin and Vinculin at an atomic resolution. These results clarified the ・-Catenin mediated tension sensing mechanism, which converts physical information into biochemical reactions represented by a molecular recognition.

Research Products

• [Journal Article] Structural basis of cargo recogniti-on bythe myosin-X MyTH4-FERM domain. (2011)
  • Author(s): Hirano,Y., Hatano,T., Takahashi,A., Toriyama,M., Inagaki,N. Hakoshima,T.
  • Journal Title: EMBO J. Volume: 30 Pages: 2734-2747
  • Peer Reviewed
• [Journal Article] 植物ホルモンによって誘導されるタンパク質分解のメカニズム (2011)
  • Author(s): 村瀬浩司, 平野良憲, 箱嶋敏雄
  • Journal Title: 実験医学 Volume: 29 Pages: 1989-1994
• [Journal Article] 植物ホルモンの受容とシグナル伝達の構造基盤 (2011)
  • Author(s): 西村宜之, 平野良憲, 人見研一, 箱嶋敏雄, 村瀬浩司
  • Journal Title: 化学と生物 Volume: 49 Pages: 161-169
  • NAID: 10027898596
• [Journal Article] Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain (2011)
  • Author(s): Hirano, Y., Hatano, T., Takahashi, A., Toriyama, M., Inagaki, N., Hakoshima, T.
  • Journal Title: EMBO J Volume: 30 Pages: 2734-2747
  • Peer Reviewed
• [Journal Article] Crystallization of the plant hormone receptors PYL9/RCAR1, PYL5/RCAR8 and PYR1/RCAR11 in the presence of (+)- abscisic acid. (2010)
  • Author(s): Shibata,N., Kagiyama,M., Nakagawa,M, Hirano,Y., Hakoshima,T.
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun Volume: 60 Pages: 456-459
  • Peer Reviewed
• [Journal Article] Mechanism of Hormone and Effector Recognition by the Gibberellin Receptor (2010)
  • Author(s): Hirano,Y., Murase,K., Hakoshima,T.
  • Journal Title: SPring-8 Research Frontiers Pages: 24-25
• [Journal Article] ジベレリンの受容と情報伝達の分子メカニズム (2010)
  • Author(s): 村瀬浩司, 平野良憲, 箱嶋敏雄
  • Journal Title: 植物の生長調節 Volume: 45 Pages: 40-48
  • NAID: 110007641952
• [Journal Article] Crystallization of the plant hormone receptors PYL9/RCAE1, PYL5/RCAR8 and PYR1/RCAR11 in the presence of (+)-abscisic acid (2010)
  • Author(s): Shibata, N., Kagiyama, M., Nakagawa, M, Hirano, Y., Hakoshima, T.
  • Journal Title: Acta Crystallogr Sect F Struct Biol Cryst Commun Volume: 66 Pages: 456-459
  • Peer Reviewed
• [Journal Article] Mechanism of Hormone and Effector Recognition by the Gibberellin Receptor (2010)
  • Author(s): Hirano, Y., Murase, K., Hakoshima, T.
  • Journal Title: SPring-8 Research Frontiers Pages: 24-25
• [Journal Article] 植物によるジベレリンの感知とシグナル伝達 (2009)
  • Author(s): 箱嶋敏雄, 平野良憲, 村瀬浩司, Tai-ping Sun
  • Journal Title: 生物物理 Volume: 49 Pages: 200-201
  • NAID: 10025975787
• [Journal Article] 植物ホルモン受容体によるシグナル伝達制御の構造研究 (2009)
  • Author(s): 平野良憲, 村瀬浩司, 箱嶋敏雄
  • Journal Title: 蛋白質核酸酵素 Volume: 54 Pages: 833-842
  • NAID: 40016675782
• [Journal Article] ジベレリン受容体GID1によるジベレリン認識とその分子進化 (2009)
  • Author(s): 箱嶋敏雄, 村瀬浩司, 平野良憲, Tai-ping Sun
  • Journal Title: 日本結晶学会誌 Volume: 52 Pages: 37-37
• [Presentation] Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain. (2011)
  • Author(s): Hirano, Y., Hatano, T., Takahashi, A., Toriyama, M., Inagaki, N., Hakoshima, T.
  • Organizer: XXII Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: マドリッド(スペイン)
  • Year and Date: 2011-08-24
• [Presentation] Structural basis of cargo recognition by the myosin-X MyTH4-FERM domain (2011)
  • Author(s): Hirano, Y., Hatano, T, Takahashi, A., Toriyama, M., Inagaki, N., Hakoshima, T.
  • Organizer: XXII Congress and General Assembly of the International Union of Crystallography
  • Place of Presentation: マドリッド(スペイン)
  • Year and Date: 2011-08-24
• [Presentation] Myosin-X のMyTH4-FERM ドメインによる積み荷認識機構の構造的基盤 (2011)
  • Author(s): 平野良憲, 畑野大樹, 高橋彩, 箱嶋敏雄
  • Organizer: 第11回 日本蛋白質科学会年会
  • Place of Presentation: 大阪
  • Year and Date: 2011-06-07
• [Presentation] ジベレリン受容体のジベレリン識別とエフェクター認識の構造的基礎 (2009)
  • Author(s): 箱嶋敏雄, 村瀬浩司, 平野良憲, Tai-ping Sun
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-10
• [Presentation] ジベレリン受容体のジベレリン識別とエフェクター認識の構造的基礎 (2009)
  • Author(s): 箱嶋敏雄, 村瀬浩司, 平野良憲, Tai-ping Sun
  • Organizer: 第32回日本分子生物学会年会 ワークショップ「翻訳後修飾・分解による細胞制御の構造生物学の最前線」
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-10
• [Presentation] 張力による細胞接着制御機構の解明に向けたαカテニンの構造研究 (2009)
  • Author(s): 平野良憲, 米村重信, 箱嶋敏雄
  • Organizer: 特定領域研究「生体超分構造」 第６回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪府)
  • Year and Date: 2009-12-02
• [Presentation] 張力による細胞接着制御機構の解明に向けたαカテニンの構造研究 (2009)
  • Author(s): 平野良憲, 米村重信, 箱嶋敏雄
  • Organizer: 特定領域研究「生体超分構造」第6回公開シンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪府)
  • Year and Date: 2009-12-02
• [Presentation] 植物ホルモン受容体によるシグナル伝達制御の構造的基盤 (2009)
  • Author(s): 平野良憲, 村瀬浩司, Tai-ping Sun, 箱嶋敏雄
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」 第５回ワークショップ
  • Place of Presentation: 湘南国際村センター, 国際会議場(神奈川県)
  • Year and Date: 2009-07-27
• [Presentation] 植物ホルモン受容体によるシグナル伝達制御の構造的基盤 (2009)
  • Author(s): 平野良憲, 村瀬浩司, Tai-ping Sun, 箱嶋敏雄
  • Organizer: 特定領域研究「生体超分子の構造形成と機能制御の原子機構」第5回ワークショップ
  • Place of Presentation: 湘南国際村センター国際会議場(神奈川県)
  • Year and Date: 2009-07-27
• [Presentation] Structure-function analysis of GA receptor and DELLA protein in Arabidopsis (2009)
  • Author(s): Murase, K., Rodolfo, Z., Hirano, Y., Hu, J., Olszewski, N., Jeong, S.Y., Hakoshima, T., Sun, T.P.
  • Organizer: Plant Biology 2009 (Joint Annual Meetings of the American Society of Plant Biologists and the Phycological Society of America)
  • Place of Presentation: ホノルル(アメリカ合衆国)
  • Year and Date: 2009-07-20
• [Presentation] Structure-function analysis of GA receptor and DELLA protein in Arabidopsis (2009)
  • Author(s): Koji Murase, Zentella Rodolfo, Yoshinori Hirano, Jianhong Hu, Neil Olszewski, Sun Yong Jeong, Toshio Hakoshima, Tai-ping Sun
  • Organizer: Plant Biology 2009 (Joint Annual Meetings of the American Society of Plant Biologists and the Phycological Society of America)
  • Place of Presentation: アメリカ合衆国(ホノルル)
  • Year and Date: 2009-07-20
• [Presentation] Mechanism of gibberellin perception and DELLA protein recognition by the Arabidopsis gibberellin receptor (2009)
  • Author(s): Murase, K., Hirano, Y., Sun, T.P., Hakoshima, T.
  • Organizer: Plant Biology 2009 (Joint Annual Meetings of the American Society of Plant Biologists and the Phycological Society of America)
  • Place of Presentation: ホノルル(アメリカ合衆国)
  • Year and Date: 2009-07-19
• [Presentation] Mechanism of gibberellin perception and DELLA protein recognition by the Arabidopsis gibberellin receptor (2009)
  • Author(s): Koji Murase, Yoshinori Hirano, Tai-ping Sun, Toshio Hakoshima
  • Organizer: Plant Biology 2009 (Joint Annual Meetings of the American Society of Plant Biologists and the Phycological Society of America)
  • Place of Presentation: アメリカ合衆国(ホノルル)
  • Year and Date: 2009-07-19
• [Presentation] 植物核内受容体の作用機構 (2009)
  • Author(s): 村瀬浩司, 平野良憲, Tai-ping Sun, 箱嶋敏雄
  • Organizer: 日本農芸化学会2009 年度大会
  • Place of Presentation: 福岡
  • Year and Date: 2009-03-28
• [Presentation] ジベレリンとその受容体、およびDELLA タンパク質複合体の結晶構造 (2009)
  • Author(s): 村瀬浩司, 平野良憲, Tai-ping Sun, 箱嶋敏雄
  • Organizer: 第50回日本植物生理学会年会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-22
• [Presentation] ジベレリン受容体GID1 によるDELLA タンパク質認識の構造的基盤 (2008)
  • Author(s): 平野良憲, 村瀬浩司, Tai-ping Sun, 箱嶋敏雄
  • Organizer: 第31回分子生物学会年会, 第81回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2008-12-12