Regulation of peroxisome matrix protein import by ubiquitin modification

• Project/Area Number: 21770117
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: Kyushu University
• Principal Investigator: OKUMOTO Kanji Kyushu University, 大学院・理学研究院, 助教 (20363319)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 細胞内小器官 / ユビキチン化 / タンパク質輸送 / 翻訳後修飾 / ペルオキシソーム

Research Abstract

Peroxisome, one of the intracellular organelle, includes various physiological functions and the deficiency causes fetal genetic diseases in human. This study clarified that import of peroxisomal matrix proteins into peroxisomes, the most important process in peroxisome biogenesis, is elaborately regulated by unique ubiquitin modification of Pex5p, a cytosolic receptor of peroxisome targeting signal type-1.

Research Products

• [Journal Article] Cysteine-ubiquitination of peroxisome-targeting-signal type 1 (PTS1)-receptor Pex5p regulates Pex5p import and export
  • Author(s): 奥本寛治、美園紗知、宮田暖、松元由依、向井悟、藤木幸夫
  • Journal Title: Traffic in press
  • Peer Reviewed
• [Presentation] Functional roles of two serine proteaases, Tysnd1 and PsLon (2010)
  • Author(s): 奥本寛治、亀谷紫、藤木幸夫
  • Organizer: in peroxisome matrix The 3rd International Symposium on Protein Community
  • Place of Presentation: 日航ホテル奈良(奈良市)
  • Year and Date: 2010-09-14
• [Presentation] Characterization of peroxisome membrane protein TMEM135/PMP52 (2010)
  • Author(s): 奥本寛治、藤木幸夫
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場(大阪市)
  • Year and Date: 2010-05-19
• [Presentation] 新規ペルオキシソーム膜タンパク質TMEM135/PMP52の機能解析 (2010)
  • Author(s): 奥本寛治、藤木幸夫
  • Organizer: 第62回日本細胞生物学会大会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-05-19
• [Presentation] The complex of RING peroxins, Pex10p and Pex12p, functions as an ubiquitin ligase for the PTS1 receptor Pex5p and regulates peroxisomal matrix protein import (2009)
  • Author(s): 奥本寛治、野田浩美、藤木幸夫
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-09
• [Presentation] The complex of RING peroxis, Pex10p and Pex12p, fuctions as an ub iquitin ligase for the PTS1 receptor Pex5p and regulates peroxisomal matrix protein import. (2009)
  • Author(s): 奥本寛治、野田浩美、藤木幸夫
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2009-12-09
• [Presentation] The complex of RING peroxins, Pex10p and Pex12p, ubiquitinates the PTS1 receptor Pex5p and regulates import of peroxisomal matrix proteins (2009)
  • Author(s): 奥本寛治、野田浩美、藤木幸夫
  • Organizer: International meeting on peroxisome research
  • Place of Presentation: Institute forSystem Biology(シアトル、米国)
  • Year and Date: 2009-11-19
• [Presentation] RING ペルオキシンPex10p-Pex12p複合体はPTS1レセプターPex5pをユビキチン化し、Pex5p のペルオキシソーム-細胞質間のシャトリングを制御する (2009)
  • Author(s): 奥本寛治、野田浩美、藤木幸夫
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋国際会議場(名古屋市)
  • Year and Date: 2009-06-03
• [Presentation] PINGペルオキシンPex10p-Pex12p複合体PTS1レセプターPex5pをユビキチン化し、Pex5pのペルオキシソーム-細胞質間のシャトリングを制御する。 (2009)
  • Author(s): 奥本寛治、野田浩美、藤木幸夫
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2009-06-03
• [Remarks] ホームページ等
  • URL: http://www.biology.kyushu-u.ac.jp/%7Etaisha/
• [Remarks]
  • URL: http://www.biology.kyushu-u.ac.jp/~taisha/
• [Remarks]
  • URL: http://www.biology.kyushu-u.ac.jp/~taisha/