| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
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| Research Abstract |
This study was the NMR structural analysis for the whole structure of human TFIIE, a member of the general transcription factors, needed for the initiation of transcription for protein coding genes. Human TFIIE is a heterodimer, consisting of an alpha and a beta subunit. Although the tendency to aggregate besides the high molecular weight (~80kDa) made the structural analysis difficult, full use of the latest NMR technologies enabled to gain structural information on three functional domains, identify widely existing intrinsically disordered regions and reveal the dynamics.
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