Three-dimensional structure of lipid-raft localized membrane protein and elucidation of the mechanism forming membrane vesicles

• Project/Area Number: 21770122
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: University of Shizuoka
• Principal Investigator: YOKOYAMA Hideshi University of Shizuoka, 薬学部, 講師 (70433208)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2009: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: 膜小胞 / ストマチン / フロチリン / 脂質ラフト / 結晶化 / X線結晶構造解析 / 膜タンパク質

Research Abstract

Stomatin and flotillin, which are involved in forming membrane vesicles, are located in lipid rafts. Until now, it is not elucidated how these proteins function. I tried to prepare proteins, human stomatin and flotillin using Escherichia coli, and to determine three-dimensional structures in order to elucidate their functions. I succeeded to get these proteins. And if I can get crystals of these proteins, I will determine the structures.

Research Products

• [Journal Article] 膜タンパク質ストマチンの三次元構造とその特異的切断プロテアーゼの機能 (2010)
  • Author(s): 横山英志
  • Journal Title: YAKUGAKU ZASSHI 130 Pages: 1289-1293
  • NAID: 130000451671
  • Peer Reviewed
• [Journal Article] 膜タンパク質ストマチンの三次元構造とその特異的切断プロテアーゼの機能 (2010)
  • Author(s): 横山英志
  • Journal Title: YAKUGAKU ZASSHI Volume: 130(10) Pages: 1289-1293
  • NAID: 130000451671
  • Peer Reviewed
• [Journal Article] Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii (2009)
  • Author(s): Yohta Kuwahara, Satoru Unzai, Takashi Nagata, Yoko Hiroaki, Hideshi Yokoyama, Ikuo Matsui, Takahisa Ikegami, Yoshinori Fujiyoshi, Hidekazu Hiroaki
  • Journal Title: Biophys.J. 97 Pages: 2034-2043
  • Peer Reviewed
• [Presentation] X-ray crystal structure and function of 1510-N protease specific for stomatin from P.horikoshii (2010)
  • Author(s): Hideshi Yokoyama
  • Organizer: JST-CNRS Joint Seminar 2010
  • Place of Presentation: Paris, France
  • Year and Date: 2010-09-22
• [Presentation] 膜タンパク質ストマチンの三次元構造とその特異的切断プロテアーゼの機能 (2010)
  • Author(s): 横山英志、藤井敏、松井郁夫
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山
  • Year and Date: 2010-03-29
• [Presentation] 膜タンパク質ストマチンの三次元構造とその特異的切断プロテアーゼの機能 (2010)
  • Author(s): 横山英志
  • Organizer: 日本薬学会第130年会
  • Place of Presentation: 岡山県桃太郎アリーナ(岡山)
  • Year and Date: 2010-03-29
• [Presentation] X-ray crystal structure of stomatin from P.horikoshii (2009)
  • Author(s): Hideshi Yokoyama
  • Organizer: JST-CNRS Joint Seminar 2009
  • Place of Presentation: Tsukuba, Japan
  • Year and Date: 2009-10-30
• [Presentation] 膜タンパク質ストマチンの特異的切断プロテアーゼの二量体構造 (2009)
  • Author(s): 横山英志
  • Organizer: 静岡県立大学2008USフォーラム
  • Place of Presentation: 静岡
  • Year and Date: 2009-08-05
• [Presentation] 膜タンパク質ストマチンの三次元構造 (2009)
  • Author(s): 横山英志
  • Organizer: 第55回日本薬学会東海支部総会・大会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-07-11
• [Presentation] 膜タンパク質ストマチンの三次元構造 (2009)
  • Author(s): 横山英志
  • Organizer: 第55回日本薬学会東海支部総会・大会
  • Place of Presentation: 愛知学院大学(名古屋)
  • Year and Date: 2009-07-11
• [Remarks] ホームページ等
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/bukka/yokoyama/index.html
• [Remarks]
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/~bukka/yokoyama/index.html
• [Remarks]
  • URL: http://w3pharm.u-shizuoka-ken.ac.jp/~bukka/yokoyama/index.html
• [Patent(Industrial Property Rights)] 新規耐熱性プロテアーゼ (2010)
  • Inventor(s): 松井郁夫、横山英志
  • Industrial Property Rights Holder: 独立行政法人産業技術総合研究所
  • Acquisition Date: 2010-01-08
• [Patent(Industrial Property Rights)] ストマチン欠失変異体 (2007)
  • Inventor(s): 松井郁夫、横山英志
  • Industrial Property Rights Holder: 独立行政法人産業技術総合研究所
  • Patent Publication Number: 2009-143857
  • Filing Date: 2009-07-02
  • Acquisition Date: 2007-12-14