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Effect of exosome-associated gangliosides on the assembly of amyloid-β protein

Research Project

Project/Area Number 21770128
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Structural biochemistry
Research InstitutionHokkaido University (2010)
National Institute for Longevity Sciences,NCGG (2009)

Principal Investigator

YUYAMA Kohei  Hokkaido University, 大学院・先端生命科学研究院, 特任助教 (80415546)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords糖質 / ガングリオシド / アルツハイマー病 / エクソソーム
Research Abstract

Alzheimer's disease is pathologically featured by extraneuronal deposition of amyloid fibrils, which are composed of amyloid-β protein (Aβ). However, it still remains to be clarified how soluble monomeric Aβ assemble into fibrils. In the present study, we found that ganglioside GM1 is release from mouse primary cortical neurons, in the association with exosome. Furthermore, the exosome-associated GM1 accelerated the assembly of synthetic Aβ in vitro.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (13 results)

All 2011 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (7 results) Remarks (1 results)

  • [Journal Article] Ganglioside GD3 monoclonal antibody-induced paxillin tyrosine phosphorylation and filamentous actin assembly in cerebellar growth cones.2011

    • Author(s)
      Yuyama K., Sekino-Suzuki N, Yamamoto N, Kasahara K.
    • Journal Title

      J.Neurochem. 116

      Pages: 845-850

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Ganglioside GD3 monoclonal antibody-induced paxillin tyrosine phosphorylation and filamentous actin assembly in cerebellar growth cones2011

    • Author(s)
      Yuyama K., Sekino-Suzuki N, Yamamoto N, Kasahara K.
    • Journal Title

      J.Neurochem.

      Volume: 116 Pages: 845-850

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sphingomyelin accumulation provides a favorable milieu for GM1 ganglioside-induced assembly of amyloid β-protein.2010

    • Author(s)
      Yuyama K., Yanagisawa K.
    • Journal Title

      Neurosci.Lett. 481

      Pages: 168-172

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sphingomyelin accumulation provides a favorable milieu for GM1 ganglioside-induced assembly of amyloid beta-protein2010

    • Author(s)
      Yuyama K., Yanagisawa K.
    • Journal Title

      Neurosci.Lett.

      Volume: 481 Pages: 168-172

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] アルツハイマー病におけるエンドサイトーシス障害2009

    • Author(s)
      湯山耕平, 柳澤勝彦
    • Journal Title

      Dementia Japan 23

      Pages: 47-54

    • Related Report
      2010 Final Research Report
  • [Presentation] 神経細胞endocytosis障害によるGM1ガングリオシド誘導性アミロイドβ蛋白質重合の促進2010

    • Author(s)
      湯山耕平
    • Organizer
      第29回日本認知症学会
    • Place of Presentation
      愛知県名古屋、ウインク愛知
    • Related Report
      2010 Final Research Report
  • [Presentation] Involvement of sphingomyelin in the generation of an endogenous seed for Alzheimer amyloid, Gaβ2010

    • Author(s)
      Kohei Yuyama
    • Organizer
      第53回日本神経化学会
    • Place of Presentation
      兵庫県神戸、神戸コンベンションセンター
    • Related Report
      2010 Final Research Report
  • [Presentation] Endocytic pathway perturbation as a putative cause of GM1-ganglioside-induced amyloid fibril formation in Alzheimer's disease.2010

    • Author(s)
      Kohei Yuyama
    • Organizer
      The 27th Naito Conference on Membrane Dynamics and Lipid Biology [I]
    • Place of Presentation
      Sapporo, Hokkaido, Japan
    • Related Report
      2010 Final Research Report
  • [Presentation] エンドサイトーシス障害によるGM1ガングリオシド蓄積とアミロイドβ白質の重合促進2010

    • Author(s)
      湯山耕平
    • Organizer
      第5回スフィンゴテラピー研究会
    • Place of Presentation
      鳥取県米子、米子市観光センター
    • Related Report
      2010 Final Research Report
  • [Presentation] エンドサイトーシス障害時におけるGM1ガングリオシド依存性アミロイド蛋白質重合2009

    • Author(s)
      湯山耕平、柳澤勝彦
    • Organizer
      日本糖質学会
    • Place of Presentation
      岐阜県高山市
    • Year and Date
      2009-09-09
    • Related Report
      2009 Annual Research Report
  • [Presentation] アルツハイマー病におけるGM1ガングリオシド誘導アミロイドβ蛋白質重合に関与する後期エンドサイトーシス障害2009

    • Author(s)
      湯山耕平
    • Organizer
      第52回日本神経化学会
    • Place of Presentation
      群馬県渋川市、ホテル天坊
    • Year and Date
      2009-06-22
    • Related Report
      2010 Final Research Report
  • [Presentation] エンドサイトーシス障害におけるGM1ガングリオシド依存性アミロイドβ蛋白質重合2009

    • Author(s)
      湯山耕平
    • Organizer
      第29回日本糖質学会年会
    • Place of Presentation
      岐阜県高山市、飛騨・世界生活文化センター
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://biomem.pharm.hokudai.ac.jp/

    • Related Report
      2010 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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