Analysis of the molecular mechanism of autophagosome formation from the viewpoint of lipid molecules
| Project/Area Number |
21770129
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
OBARA Keisuke Hokkaido University, 大学院・薬学研究院, 助教 (30419858)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2009: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 脂質 / タンパク質 / オートファジー / タンパク質分解 / スフィンゴ / スフィンゴ脂質 / オートファゴソーム / 脂肪酸 / 酵母 / 液胞 |
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| Research Abstract |
In autophagy, sequestration of cytoplasmic contents by a newly formed double-membrane structure called the autophagosome is the most fundamental process. Lipid composition of the autophagosome is still largely unknown ; but form electron microscopic analyses, it has been speculated that the autophagosome is rich in some unusual lipids such as sphingolipids. In this study, we discovered that sphingolipid synthesis is required for proper progression of autophagy. We also suggested that inositolphosphoryl ceramide is involved in autophagy among several sphingolipid species in yeast. We next tried to reveal the process in which sphingolid is actually involved. Two ubiquitin-like conjugating reactions are essential for autophagy. Sphingolipid was not essential for these reactions. Sphingolipid synthesis was not required for the construction of the PAS a peri-vacuolar structure where autophagy-related proteins assemble, either. Maturation of vacuolar hydrolytic enzymes was not affected by inhibiting sphingolipid synthesis. Sphingolipid is considered to be involved in other processes in autophagy.
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Report
(3 results)
Research Products
(5 results)
