Structural and biochemical analysis of Sec translocon machinery
| Project/Area Number |
21770135
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | タンパク質 / 膜透過 / 膜タンパク質 / 膜組込 / トランスロコン / Sec / 構造解析 / 構造生物学 / 蛋白質 / トランスコロン / 膜蛋白質 |
|---|
| Research Abstract |
Transport of proteins across membranes is one of the fundamental and essential cellular activities in all organisms. We solved the first 3D structure of a membrane component SecDF, which plays an important role in efficient translocation of newly synthesized proteins across the membrane in bacteria. From the structural features and functional analyses, we proposed that SecDF functions as a membrane-integrated chaperone, which drives movement of proteins without using a major currency, ATP, of energy, but with remarkable cycles of conformational changes, powered by a proton H+ion) gradient across the membrane. Our model of the SecDF function has been verified by a series of biochemical and biophysical approaches. This research uncovers a new, atomic-level principle that underlies the cellular protein delivery systems in particular and biological mechanisms of active transport in general, by which cells acquire or dispose not only macromolecules but also ions, drugs and nutrients.
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Report
(4 results)
Research Products
(40 results)
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[Journal Article] Crystal structure of the channelrhodopsin light-gated cation channel2012
Author(s)
Hideaki E.Kato, Feng Zhang, Ofer Yizhar, Charu Ramakrishnan, Tomohiro Nishizawa, Kunio Hirata, Jumpei Ito, Yusuke Aita, Tomoya Tsukazaki, Shigehiko Hayashi, Peter Hegemann, Andres D.Maturana, Ryuichiro Ishitani, Karl Deisseroth, Osamu Nureki
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Journal Title
Nature
Volume: 482
Issue: 7385
Pages: 369-374
DOI
Related Report
Peer Reviewed
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[Journal Article] Crystallization and preliminary X-ray diffraction of the first periplasmic domain of SecDF, a translocon-associated membrane protein, from Thermus thermophilus2011
Author(s)
Echizen, Y., Tsukazaki, T., Dohmae, N., Ishitani, R. and Nureki, O
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Journal Title
Acta. Crystallograph. Sect. F Struct. Biol. Cryst. Commun
Volume: 67
Pages: 1367-1370
URL
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[Journal Article] Mg^<2+>-dependent gating ofbacterial MgtE channel underlies Mg^<2+> homeostasis2009
Author(s)
Hattori, M., Iwase, N., Furuya, N., Tanaka, Y., Tsukazaki, T., Ishitani, R., Maguire, M. E., Ito, K. and Nureki, O
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Journal Title
EMBO J
Volume: 28
Pages: 3602-361
URL
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