| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Previously we demonstrated that COUP-TF nuclear receptors contribute to the establishment of dorso-ventral expression pattern of cone opsins in mouse retina. In this study I'd like to clarify the mechanism of this regulation by focusing on epigenetic mechanism. I analyzed DNA methylation status of dorsal- and ventral-region of mouse retina, or DNA methylation and histone modification status in retinoblastoma cell line in which endogenous COUP-TFs were knocked down by shRNA. However, significant correlation between COUP-TFs expression and these epigenetic status has not been seen yet. On the other hand, I identified TRbeta2 regulating genomic region in human red opsin gene and found that COUP-TFs show the different effect on TRbeta2-mediated activation in this genomic region between human and mouse genome. Furthermore, I found that Brg1, a chromatin remodeling factor, activates blue opsin gene expression and COUP-TFs suppress this Brg1-mediated activation. These results suggest that COUP-TFs regulate cone opsin expression in retina through interaction of these factors, which establishes each animal specific cone opsin expression pattern.
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