Studies on regulation of transport vesicle budding from endoplasmic reticulum by the calcium-binding protein ALG-2
| Project/Area Number |
21770139
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | Nagoya University |
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Principal Investigator |
SHIBATA Hideki 名古屋大学, 生命農学研究科, 准教授 (30314470)
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| Research Collaborator |
MAKI Masatoshi 名古屋大学, 大学院・生命農学研究科, 教授 (40183610)
WADA Ikuo 福島県立医科大学, 医学部, 教授 (40182969)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2009: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 細胞情報伝達機構 / 小胞輸送 / カルシウム / COPII 小胞 / タンパク質問相互作用 / 蛍光イメージング / シグナル伝達 / タンパク質間相互作用 / COPII小胞 / アネキシン / FRAP / カルシウム結合タンパク / 小胞体 / ALG-2 |
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| Research Abstract |
Sec31A is an outer component of COPII coat of endoplasmic reticulum (ER)-to-Golgi transport vesicles. We previously found that the penta-EF-hand protein ALG-2 calcium-dependently interacts with Sec31A. In this study, we first narrowed the Sec31A region responsible for ALG-2 binding to 13 amino acids. Next, fluorescence recovery after photobreaching analysis revealed that the ALG-2 binding region constitutes a high affinity binding site of Sec31A for the ER membrane. Finally, the calcium/phospholipid binding protein annexin All was identified as a recruiting protein to the budding site of COPII by the adaptor function of ALG-2.
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Report
(3 results)
Research Products
(30 results)
