Identification of genes involved in transport of GPI-anchored proteins

• Project/Area Number: 21770140
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: FUJITA Morihisa Osaka University, 微生物病研究所, 特任助教 (30532056)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 糖鎖生物学 / タンパク質輸送 / 糖鎖 / 翻訳後修飾 / 細胞内輸送 / 糖脂質 / GPIアンカー / GPIアンカー型タンパク質

Research Abstract

Glycosylphosphatidylinositol (GPI) anchoring of proteins is a post-translational modification occurring in the endoplasmic reticulum (ER). Here, we established mutant cell lines that were selectively defective in transport of GPI-anchored proteins from the ER to the Golgi. We identified a responsible gene, designated PGAP5 (post-GPI-attachment to proteins 5). PGAP5 catalyzed the remodeling of the glycan moiety on GPI-APs. Our data demonstrate that GPI glycan acts as an ER-exit signal and suggest that glycan remodeling mediated by PGAP5 regulates GPI-AP transport in the early secretory pathway

Research Products

• [Journal Article] Biogenesis of GPI-anchored proteins is essential for surface expression of sodium channels in zebrafish Rohon-Beard neurons to respond to mechanosensory stimulation. (2010)
  • Author(s): Yuri Nakano, Morihisa Fujita, Kazutoyo Ogino, Louis Saint-Amant, Taroh Kinoshita, Yoichi Oda, Hiromi Hirata.
  • Journal Title: Development 137 Pages: 1689-1698
  • Peer Reviewed
• [Journal Article] Structural remodeling of GPI anchors during biosynthesis and after attachment to proteins. (2010)
  • Author(s): Morihisa Fujita, Taroh Kinoshita.
  • Journal Title: FEBS Lett. 584 Pages: 1670-1677
  • Peer Reviewed
• [Journal Article] Biogenesis of GPI-anchored proteins is essential for surface expression of sodiumchannels in zebrafish Rohon-Beard neurons to respond to mechanosensory stimulation (2010)
  • Author(s): 中野由梨、藤田盛久
  • Journal Title: Development Volume: 137 Pages: 1689-1698
  • Peer Reviewed
• [Journal Article] GPI-Anchor : Update for Biosynthesis and Remodeling (2010)
  • Author(s): 前田裕輔、藤田盛久
  • Journal Title: Trends in Glycoscience and Glycotechnology Volume: 22 Pages: 182-193
  • NAID: 10027892890
  • Peer Reviewed
• [Journal Article] GPI-glycan remodeling by PGAP5 regulates transport of GPI-anchored proteins from the ER to the Golgi. (2009)
  • Author(s): Morihisa Fujita, Yusuke Maeda, Moonjin Ra, Yoshiki Yamaguchi, Ryo Taguchi, Taroh Kinoshita.
  • Journal Title: Cell 139 Pages: 352-365
  • Peer Reviewed
• [Journal Article] GPI glycan remodeling by PGAP5 regulates transport of GPI-anchored proteins from the ER to the Golgi (2009)
  • Author(s): 藤田盛久
  • Journal Title: Cell 139 Pages: 352-365
  • Peer Reviewed
• [Journal Article] Structural remodeling of GPI anchors during biosynthesis and after attachment toproteins (2009)
  • Author(s): 藤田盛久
  • Journal Title: FEBS Letters (in press)
  • Peer Reviewed
• [Presentation] 小胞体におけるGPIアンカー型タンパク質の選別輸送機構 (2010)
  • Author(s): 藤田盛久、前田裕輔、木下タロウ
  • Organizer: BMB2010
  • Place of Presentation: 神戸市(兵庫県)
  • Year and Date: 2010-12-07
• [Presentation] Molecular ticket for transport of GPI-anchored proteins from the ER : Transient modification by a side-chain ethanolamine phosphate on GPI-glycan (2010)
  • Author(s): 藤田盛久
  • Organizer: The 25th International Carbohydrate Symposium
  • Place of Presentation: 千葉市(千葉県)
  • Year and Date: 2010-08-03
• [Presentation] Structural remodeling and selective transport of GPI-anchored proteins (2010)
  • Author(s): 藤田盛久
  • Organizer: The Joint Symposium of the 5th International Symposium of Institute Network
  • Place of Presentation: 金沢市(石川県)
  • Year and Date: 2010-06-24
• [Presentation] Sorting, trafficking and remodeling of GPI-anchored proteins (2010)
  • Author(s): Morihisa Fujita
  • Organizer: The 4th International Symposium of WPI IFReC "Immunology at the Forefront"
  • Place of Presentation: 吹田市(大阪府)
  • Year and Date: 2010-06-02
• [Presentation] Sorting, trafficking and remodeling of GPI-anchored proteins (2010)
  • Author(s): 藤田盛久
  • Organizer: The 4th International Symposium of WPI IFReC "Immunology at the Forefront"
  • Place of Presentation: 吹田市(大阪府)(招待講演)
  • Year and Date: 2010-06-02
• [Presentation] Sorting of GPI-anchored proteins at the ER-exit site (2010)
  • Author(s): Morihisa Fujita, Taroh Kinoshita
  • Organizer: EMBO conference series : Towards a comprehensive understanding of endoplasmic reticulum functions
  • Place of Presentation: Girona (Spain)
• [Presentation] Structural remodeling and selective transport of GPI-anchored proteins (2010)
  • Author(s): 藤田盛久
  • Organizer: International Symposium on Organelle Network : Interface among Infection-Immunity, Cell Biology and Glycobiology
  • Place of Presentation: 豊中市(大阪府)
• [Presentation] Sorting of GPI-anchored proteins at the ER-exit site (2010)
  • Author(s): 藤田盛久
  • Organizer: EMBO conference series : Towards a comprehensive understanding of endoplasmic reticulum functions
  • Place of Presentation: ジローナ(スペイン)
• [Presentation] GPIアンカー型タンパク質の輸送を制御する糖鎖リモデリング (2009)
  • Author(s): 藤田盛久
  • Organizer: 第7回糖鎖科学コンソーシアムシンポジウム
  • Place of Presentation: 豊中市(大阪府)
  • Year and Date: 2009-12-08
• [Presentation] GPIアンカー型タンパク質の輸送を制御する糖鎖リモデリング (2009)
  • Author(s): 藤田盛久
  • Organizer: 第7回 糖鎖科学コンソーシアムシンポジウム
  • Place of Presentation: 千里ライフサイエンスセンター(大阪府)
  • Year and Date: 2009-12-08
• [Presentation] GPIアンカーの構造変化によるタンパク質輸送の制御 (2009)
  • Author(s): 藤田盛久、前田裕輔、羅紋眞、山口芳樹、田口良、木下タロウ
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸市(兵庫県)
  • Year and Date: 2009-10-22
• [Presentation] GPIアンカーの構造変化によるタンパク質輸送の制御 (2009)
  • Author(s): 藤田盛久
  • Organizer: 第82回 日本生化学会大会
  • Place of Presentation: 神戸ポートアイランド(兵庫県)
  • Year and Date: 2009-10-22
• [Presentation] GPIアンカー型タンパク質の細胞内輸送を制御する糖鎖リモデリング (2009)
  • Author(s): 藤田盛久
  • Organizer: 第61回 細胞生物学会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-06-04
• [Presentation] GPI-glycan remodeling by PGAP5 regulates transport of GPI-anchored proteins from the ER (2009)
  • Author(s): Morihisa Fujita, Yusuke Maeda, Moonjin Ra, Yoshiki Yamaguchi, Ryo Taguchi, Taroh Kinoshita
  • Organizer: FASEB Summer Research Conferences : Protein lipidation, signaling and membrane domains
  • Place of Presentation: Saxtons River, Vermont(USA)
• [Presentation] GPI-glycan remodeling by PGAP5 regulates transport of GPI-anchored proteins from the ER (2009)
  • Author(s): 藤田盛久
  • Organizer: FASEB Summer Research Conferences : Protein lipidation, signaling and membrane domains
  • Place of Presentation: SAXTONS RIVER, VERMONT (USA)
• [Book] 実験医学増刊・分子から個体へと深化する脂質生物学(GPI付加によるタンパク質選別輸送・局在の調節) (2010)
  • Author(s): 藤田盛久、木下タロウ
• [Book] 実験医学(GPIアンカーの脂質・糖鎖リモデリング)(Vol.28, No.8) (2010)
  • Author(s): 神澤範行、藤田盛久、木下タロウ
• [Book] 実験医学Vol.28, No.8 (2010)
  • Author(s): 神澤範行、藤田盛久
  • Publisher: GPIアンカーの脂質・糖鎖リモデリング
• [Book] 実験医学増刊・分子から個体へと深化する脂質生物学 (2010)
  • Author(s): 藤田盛久
  • Publisher: GPI付加によるタンパク質選別輸送・局在の調節
• [Remarks] ホームページ等
  • URL: http://www.biken.osaka-u.ac.jp/biken/men-eki-huzen/