| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
We have previously demonstrated that Src tyrosine kinase plays an important role in the Xenopus egg activation. However, the molecular mechanism of egg activation is still unclear. Here, we investigated the activation mechanism and the physiological function of Src kinase in Xenopus egg fertilization. Biochemical and immunochemical analyses have revealed the involvement of heterotrimeric G proteins in sperm-induced Src activation. Moreover, we analyzed hnRNP K protein, which is known as a substrate of Src kinase in Xenopus egg fertilization. Our molecular biological and biochemical analyses showed that the hnRNP K binds to specific mRNAs in unfertilized egg, and that Src-mediated tyrosine phosphorylation acts as a trigger for their dissociation at egg activation.
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