Mechanisms involved in ghrelin degradation in circulation and identification of its processing enzymes.

• Project/Area Number: 21770149
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Dokkyo Medical University
• Principal Investigator: SATOU Motoyasu 獨協医科大学, 医学部, 助教 (20418891)
• Research Collaborator: NISHI Yoshihiro 久留米大学, 医学部, 講師 (20352122)
• Project Period (FY): 2009 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: グレリン / 酵素 / ペプチドホルモン / 脂質修飾 / タンパク質精製 / 血液 / 食欲 / プロテインC

Research Abstract

A peptide hormone ghrein has divers physiological actions including growth hormone release, appetite control and immunomodulation. In this study, the ghrelin deacylation enzyme (APT1) and two ghrelin peptidases (APC and TAFI) were purified from animal blood, and their cDNAs were isolated. The enzymatic processed formsshowed synergistic or distinctive roles in ghrelin actions such as cell differentiation.

Research Products

• [Journal Article] The study of ghrelin deacylation enzymes (2012)
  • Author(s): Satou M, Sugimoto, H.
  • Journal Title: Methods Enzymol Volume: 514 Pages: 165-179
  • DOI: 10.1016/b978-0-12-381272-8.00011-8
  • Peer Reviewed
• [Journal Article] Transmembrane topology and oligomeric structure of the high-affinity choline transporter. (2012)
  • Author(s): Okuda, T.
  • Journal Title: The Journal of biological chemistry Volume: 287 Issue: 51 Pages: 42826-42834
  • DOI: 10.1074/jbc.m112.405027
  • Peer Reviewed
• [Journal Article] Understanding the functional significance of ghrelin processing and degradation (2011)
  • Author(s): Satou M, Nakamura Y, Ando H, Sugimoto H
  • Journal Title: Peptides Volume: 32 Issue: 11 Pages: 2183-2190
  • DOI: 10.1016/j.peptides.2011.06.029
  • Peer Reviewed
• [Journal Article] The heteromeric G protein subunits Gαq and Gβ1 have lysophospholipase D activity (2011)
  • Author(s): Aoyama C, Sugimoto H, Ando H, Yamashita S, Horibata Y, Sugimoto S, Satou M
  • Journal Title: Biochem J Volume: 440 Issue: 2 Pages: 241-250
  • DOI: 10.1042/bj20110545
  • Peer Reviewed
• [Journal Article] Identification and characterization of Acyl-protein thioesterase1/lysophospholipase I as a ghrelin deacylation/lysophospholipid hydrolyzing enzyme in fetal bovine serum and conditioned medium (2010)
  • Author(s): Satou M, Nishi Y, Yoh J,Hattori, Y, Sugimoto H
  • Journal Title: Endoclinol Volume: 151(10) Issue: 10 Pages: 4765-4775
  • DOI: 10.1210/en.2010-0412
  • Peer Reviewed
• [Journal Article] Identification and characterization of Acyl-protein thioesterase1/lysophospholipase I as a ghrelin deacylation/lysophospholipid hydrdlyzing enzyme in fetsl bovine serum and conditioned medium. (2010)
  • Author(s): Satou M., et al.
  • Journal Title: Endocrinotogy Volume: 151 Pages: 4765-4775
  • Peer Reviewed
• [Journal Article] Regional distribution and dynamics of n-decanoylghrelin, another acyl-form of ghrelin, upon fasting in rodents. (2009)
  • Author(s): Himejima H., et al.
  • Journal Title: Regulatory Peptides Vol.156 Pages: 47-59
  • Peer Reviewed
• [Presentation] 血中グレリン切断酵素の精製と分解産物の生理活性 (2012)
  • Author(s): 佐藤元康、西芳寛、杉本博之
  • Organizer: 第54回日本脂質生化学会
  • Place of Presentation: 福岡
  • Year and Date: 2012-06-08
• [Presentation] Lysophospholipase1(Lypla1)/Acylprotein thioesterase 1(APT1)のプロモーター解 析による転写調節機構の解明 (2012)
  • Author(s): 中村祐介
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡市
• [Presentation] 血中グレリン切断酵素の精製と分解産物の生理活性 (2012)
  • Author(s): 佐藤元康
  • Organizer: 第54日本脂質生化学会
  • Place of Presentation: 福岡市
• [Presentation] 25-OHコレステロールによるCTP : ホスホエタノールアミン・シチジリルトランスフェラーゼ(ET)の転写抑制における分子機構の解析 (2012)
  • Author(s): 安戸博美
  • Organizer: 第54日本脂質生化学会
  • Place of Presentation: 福岡市
• [Presentation] PC12細胞の神経突起伸長におけるアシルプロテインチオエステラーゼ1の機能的役割 (2011)
  • Author(s): 佐藤元康、西芳寛、杉本博之
  • Organizer: 第84回日本生化学会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-24
• [Presentation] PC12細胞の神経突起伸長におけるアシルプロテインチオエステラーゼ1の機能的役割 (2011)
  • Author(s): 佐藤元康, 他
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-24
• [Presentation] 細胞内リゾホスホリパーゼD活性と三量体Gタンパク質との関連 (2011)
  • Author(s): 青山智英子, 他
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-22
• [Presentation] 25-OHコレステロールによるCTP:ホスホエタノールアミン・シチジリルトランスフェラーゼ(ET)の転写抑制における分子機構の解析 (2011)
  • Author(s): 安戸博美, 他
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-22
• [Presentation] Mechanism of supression of transcription and enzyme activity of CTP : Phosphoethanolamine cytidylyltransferase in vitro by Low-density lipoprotein and oxysterols. (2010)
  • Author(s): Ando H., et al.
  • Organizer: 第83回日本生化学会大会・第33日本分子生物学会年会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-10
• [Presentation] グレリン受容体を介したEGR-1遺伝子の活性化に対するPI3K非依存的なLY294002の効果 (2010)
  • Author(s): 佐藤元康、西芳寛、杉本博之
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Identification of Novel Lysophospholipase D from Rat Brain. (2010)
  • Author(s): Aoyama C., et al.
  • Organizer: 第83回日本生化学会大会・第33日本分子生物学会年会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-09
• [Presentation] グレリン受容体を介したEGR-1遺伝子の活性化に対するPI3K非依存的なLY294002の効果 (2010)
  • Author(s): 佐藤元康
  • Organizer: 第83回日本生化学会大会・第33日本分子生物学会年会合同大会
  • Place of Presentation: 神戸市
  • Year and Date: 2010-12-07
• [Presentation] 活性型プロテインCによる短縮グレリン(1-15)の生成とその生理活性 (2009)
  • Author(s): 佐藤元康・西芳寛・杉本博之
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Presentation] Identification of truncated and partially active form of ghrelin (1-15) generated by activated protein C in bovine plasma (2009)
  • Author(s): 佐藤元康
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2009-12-12
• [Presentation] 血中グレリン脱アシル化酵素としてのリゾホスホリパーゼIの役割 (2009)
  • Author(s): 佐藤元康
  • Organizer: 第51回日本脂質生化学会
  • Place of Presentation: ウィル愛知(名古屋市)
  • Year and Date: 2009-07-30
• [Presentation] デカン酸修飾型グレリンの分泌動態の検討:肥満度、インスリン感受性との関連 (2009)
  • Author(s): 葉純子
  • Organizer: 第82回日本内分泌学会学術総会
  • Place of Presentation: 群馬県民会館(前橋市)
  • Year and Date: 2009-04-23
• [Book] Read & Researchmap
  • URL: http://researchmap.jp/satoumdokkyomed/