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Regulation of receptor trafficking and cytoskeleton by Arf small G proteins and their effectors

Research Project

Project/Area Number 21770150
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research InstitutionTokyo University of Pharmacy and Life Science

Principal Investigator

INOUE Hiroki  Tokyo University of Pharmacy and Life Science, 生命科学部, 講師 (10294448)

Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords膜輸送と輸送タンパク質 / 膜輸送 / 細胞骨格 / エンドサイトーシス / ホスホリパーゼA1 / Arf G protein / Rab G protein / WAVE複合体
Research Abstract

ASAP1 and FIP3 are effectors and/or regulators of Arf and Rab small G proteins. In this study, the possibility that they are involved in actin cytoskeleton regulation and endocytosis of growth factor receptor was evaluated. For the purpose of this, circular dorsal ruffles formation and PDGF receptor endocytosis was used as a model system. ASAP1 has been proposed to be a negative regulator for CDR formation because its overexpression down -regulates CDR formation. In this work, FI P3, which is an ASAP1-interacting protein, was identified as a positive regulator for CDR formation and PDGF endocytosis. Some of Arf and Rab proteins were localized in CDRs. Taken together, FIP3 may play a role in a crosstalk between actin remodeling and receptor endocytosis as one of key molecules.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (7 results)

All 2010 2009 Other

All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Golgi-localized KIAA0725p regulates membrane trafficking from the Golgiapparatus to the plasma membrane in mammalian cells.2010

    • Author(s)
      Sato, S.,^* Inoue, H.,^* Kogure, T., Tagaya, M., Tani, K.(^*, These authors contributed equally to this work.)
    • Journal Title

      FEBS lett. 584

      Pages: 4389-4395

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Golgi-localized KIAA0725p regulates membrane trafficking from the Golgi apparatus to the plasma membrane in mammalian cells.2010

    • Author(s)
      Sato, S., Inoue, H., Kogure, T., Tagaya, M., Tani, K.
    • Journal Title

      FEBS lett.

      Volume: 584 Pages: 4389-4395

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Ciliary targeting motif VxPx directs assembly of a trafficking module through Arf4.2009

    • Author(s)
      Mazelova, J., Astuto-Gribble, L., Inoue, H., Tam, B.M., Schonteich, E., Prekeris, R., Morit,z O.L., Randazzo, P.A., Deretic, D.
    • Journal Title

      EMBO J. 28

      Pages: 183-192

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Presentation] KIAA0725p/DDHD2, a mammalian Golgi-associated phospholipase A_1, regulates membrane trafficking from the Golgi apparatus to the plasma membrane.2010

    • Author(s)
      Inoue, H., Sato, S., Kogure, T., Arimitsu, N., Tagaya, M., Tani, K.
    • Organizer
      Sapporo International Cancer Symposium 2010 "Membranetraffic and Cancer"
    • Place of Presentation
      札幌
    • Related Report
      2010 Annual Research Report
  • [Presentation] Sec16Bはミトコンドリアおよびペルオキシソームの形態形成に関与する2009

    • Author(s)
      米川周佑、中島寿基、井上弘樹、多賀谷光男、谷佳津子
    • Organizer
      日本生化学会 第82回大会
    • Place of Presentation
      神戸
    • Related Report
      2010 Final Research Report
  • [Presentation] Sec16Bはミトコンドリアおよびペルオキシソームの形態形成に関与する.2009

    • Author(s)
      米川周佑
    • Organizer
      日本生化学会第82回大会
    • Place of Presentation
      神戸
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.ls.toyaku.ac.jp/Life-Science/lmcb-6/

    • Related Report
      2010 Final Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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