| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
ClpB can rescue the aggregated proteins(disaggregation). When the motion of N domain was restricted by the disulfide bond, the disaggregation activity of ClpB reduced. We found that the cause is in the threading-through process following the substrate-binding process. Moreover, we made two ClpB mutants in which two or six adjacent subunits of ClpB hexamer were cross-linked by the disulfide bond(s). Using these mutants, we found that the efficient disaggregation by ClpB required the moderate dissociation/association of the hexamer.
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