The role of the ubiquitin-proteasome pathway in heme oxygenase system
| Project/Area Number |
21770152
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Functional biochemistry
|
|---|
| Research Institution | Kurume University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
|---|
| Keywords | ヘムオキシゲナーゼ / 小胞体関連分解(ERAD) / ユビキチン / 小胞体関連分解 |
|---|
| Research Abstract |
The present study investigated the cellular mechanism underlying the degradation of heme oxygenase(HO), an endoplasmic reticulum-anchored protein. High molecular weight ubiquitin conjugates were co-immunoprecipitated with HO from HEK293 cells after proteasome inhibition, and HO ubiquitination was confirmed in HEK293 cells overexpressing His-tagged HO and HA-tagged ubiquitin. HO specific ubiquitine-ligase was also identified using mass spectrometry.
|
Report
(4 results)
Research Products
(82 results)
-
[Journal Article] Identification of essential amino acid residues involved in the interaction of heme oxygenase-1 with caveolin-12012
Author(s)
Taira, J., Sugishima, M., Kida, Y., Oda, E., Noguchi, M., and Higashimoto, Y.
-
Journal Title
Peptide Science
Volume: 48
Pages: 35-38
NAID
Related Report
Peer Reviewed
-
-
-
-
[Journal Article] Caveolin-1 is a competitive inhibitor of heme oxygenase-1(H0-1) with heme : Identification of a minimum sequence in caveolin-1 for binding to H0-12011
Author(s)
Taira, J., Sugishima, M., Kida, Y., Oda, E., Noguchi, M., and Higashimoto, Y.
-
Journal Title
Biochemistry
Volume: 50
Pages: 6824-6831
Related Report
Peer Reviewed
-
-
-
[Journal Article] Modifications on amphiphilicity and cationicity of unnatural amino acid containing peptides for the improvement of antimicrobial activity against pathogenic bacteria2010
Author(s)
Taira, J., Kida, Y., Yamaguchi, H., Kuwano, K., Higashimoto, Y. and Kodama, H.
-
Journal Title
J. Pept. Sci
Volume: 16
Pages: 607-612
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
