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Functional analysis of heparan sulfate fine structures in Drosophila axon guidance

Research Project

Project/Area Number 21770154
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research Institution財団法人東京都医学総合研究所 (2011)
Tokyo Metropolitan Organization for Medical Research (2009-2010)

Principal Investigator

KAMIMURA Keisuke  財団法人東京都医学総合研究所, 脳発達・神経再生研究分野, 主席研究員 (30529524)

Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywordsヘパラン硫酸プロテオグリカン / ショウジョウバエ / ヘパラン硫酸 / プロテオグリカン / シナプス形成 / Wnt / ショウジョウバェ
Research Abstract

We have established the "glyocogenetics" approach, which allow the manipulation of heparan sulfate fine structures at will, using Drosophila, for which sophisticated genetic techniques are available. We also found that HSPGs have important roles in synapse formation.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (23 results)

All 2011 2010 2009 Other

All Journal Article (7 results) (of which Peer Reviewed: 6 results) Presentation (15 results) Remarks (1 results)

  • [Journal Article] In vivo manipulation of heparan sulfate structure and its effect on Drosophila development2011

    • Author(s)
      Keisuke Kamimura, Nobuaki Maeda, and Hiroshi Nakato
    • Journal Title

      Glycobiology

      Volume: 21(5) Issue: 5 Pages: 607-618

    • DOI

      10.1093/glycob/cwq202

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Functions of chondroitin sulfate and heparan sulfate in the developing brain2011

    • Author(s)
      Nobuaki Maeda, Maki Ishi, Kazunari Nishimura, and Keisuke Kamimura
    • Journal Title

      Neurochem Res

      Volume: 36(7) Issue: 7 Pages: 1228-1240

    • DOI

      10.1007/s11064-010-0324-y

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] In vivo manipulation of heparan sulfate structure and its effect on Drosophila development.2011

    • Author(s)
      Keisuke Kamimura, Nobuaki Maeda, Hiroshi Nakato
    • Journal Title

      Glycobiology

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation2010

    • Author(s)
      Adam Kleinschmit, Takashi Koyama, Katsufumi Dejima, Yoshiki Hayashi, Keisuke Kamimura and Hiroshi Nakato
    • Journal Title

      Dev. Biol

      Volume: 345(2) Issue: 2 Pages: 204-214

    • DOI

      10.1016/j.ydbio.2010.07.006

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Opposing functions of chondroitin sulfate and heparan sulfate during early neuronal polarization2010

    • Author(s)
      Kazunari Nishimura, Maki Ishii, Mutsuki Kuraoka, Keisuke Kamimura, and Nobuaki Maeda
    • Journal Title

      Neuroscience

      Volume: 169(4) Issue: 4 Pages: 1535-1547

    • DOI

      10.1016/j.neuroscience.2010.06.027

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Drosophila heparan sulfate 6-O endosulfatase regulates Wingless morphogen gradient formation.2010

    • Author(s)
      Adam Kleinschmit, Takashi Koyama, Katsufumi Dejima, Yoshiki Hayashi, Keisuke Kamimura, Hiroshi Nakato
    • Journal Title

      Developmental Biology

      Volume: 345 Pages: 204-214

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Regulation of the Function of Sonic Hedgehog by Hepacan Sulfate Proteoglycan2009

    • Author(s)
      Keisuke Kamimura
    • Journal Title

      Trends in Glycoscience and Glycotechnology Vol.21, No.122

      Pages: 354-356

    • Related Report
      2009 Annual Research Report
  • [Presentation] Perlecan regulates Wingless signaling at the Drosophila neuromuscular junction2011

    • Author(s)
      Keisuke Kamimura and Nobuaki Maeda
    • Organizer
      7^<th> International Conference on Proteoglycans
    • Place of Presentation
      Sydney, Australia
    • Year and Date
      2011-10-20
    • Related Report
      2011 Final Research Report
  • [Presentation] Perlecan regulates Wingless signaling at the Drosophila neuromuscular junction2011

    • Author(s)
      Keisuke Kamimura, Nobuaki Maeda
    • Organizer
      7th International Conference on Proteoglycans
    • Place of Presentation
      Sydney, Australia(招待講演)
    • Year and Date
      2011-10-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] ショウジョウバエの神経筋シナプス形成においてパールカンはWntシグナルを調節する2011

    • Author(s)
      神村圭亮、前田信明
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都府京都市
    • Year and Date
      2011-09-24
    • Related Report
      2011 Final Research Report
  • [Presentation] ショウジョウバエの神経筋シナプス形成においてパールカンはWntシグナルを調節する2011

    • Author(s)
      神村圭亮、前田信明
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都府京都市(招待講演)
    • Year and Date
      2011-09-24
    • Related Report
      2011 Annual Research Report
  • [Presentation] ショウジョウバエの神経筋接合部においてパールカンはWinglessシグナル伝達を調節する2011

    • Author(s)
      神村圭亮、前田信明
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      神奈川県横浜市
    • Year and Date
      2011-09-15
    • Related Report
      2011 Final Research Report
  • [Presentation] ショウジョウバエの神経筋接合部においてパールカンはWinglessシグナル伝達を調節する2011

    • Author(s)
      神村圭亮、前田信明
    • Organizer
      第34回日本神経科学大会
    • Place of Presentation
      神奈川県横浜市(招待講演)
    • Year and Date
      2011-09-15
    • Related Report
      2011 Annual Research Report
  • [Presentation] ショウジョウバエの神経筋シナプス形成におけるヘパラン硫酸プロテオグリカンの役割2011

    • Author(s)
      神村圭亮、前田信明
    • Organizer
      第29回日本糖質学会大会
    • Place of Presentation
      岐阜県高山市
    • Year and Date
      2011-09-10
    • Related Report
      2011 Final Research Report
  • [Presentation] Perlecan regulates BMP signaling essential for synaptic growth2011

    • Author(s)
      神村圭亮、前田信明
    • Organizer
      第28回内藤コンファレンス「糖鎖の発現と制御[I]-機能から病態まで-」
    • Place of Presentation
      神奈川県湘南国際村センター
    • Year and Date
      2011-07-28
    • Related Report
      2011 Final Research Report
  • [Presentation] In vivo manipulation of heparan sulfate structure and its effects on Drosophila development2010

    • Author(s)
      神村圭亮、前田信明、中藤博志
    • Organizer
      BMB2010
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2010-12-09
    • Related Report
      2011 Final Research Report
  • [Presentation] In vivo manipulation of heparan sulfate structure and its effects on Drosophila development.2010

    • Author(s)
      神村圭亮、前田信明、中藤博志
    • Organizer
      BMB2010
    • Place of Presentation
      神戸ポートアイランド
    • Year and Date
      2010-12-09
    • Related Report
      2010 Annual Research Report
  • [Presentation] ショウジョウバエの神経筋シナプス形成においてパールカンはBMPシグナルを調節する2010

    • Author(s)
      神村圭亮、前田信明
    • Organizer
      Neuro2010
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2010-09-03
    • Related Report
      2011 Final Research Report
  • [Presentation] ショウジョウバエの神経筋シナプス形成においてパールカンはBMPシグナルを調節する2010

    • Author(s)
      神村圭亮、前田信明
    • Organizer
      Neuro2010
    • Place of Presentation
      神戸コンベンションセンター
    • Year and Date
      2010-09-03
    • Related Report
      2010 Annual Research Report
  • [Presentation] ショウジョウバエの神経筋シナプス形成におけるヘパラン硫酸プロテオグリカンの機能解析2009

    • Author(s)
      神村圭亮、前田信明
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      兵庫県神戸市
    • Year and Date
      2009-10-24
    • Related Report
      2011 Final Research Report
  • [Presentation] ショウジョウバエの神経筋シナプス形成におけるヘパラン硫酸プロテオグリカンの機能解析2009

    • Author(s)
      神村圭亮、前田信明
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸国際会議場(兵庫県)
    • Year and Date
      2009-10-24
    • Related Report
      2009 Annual Research Report
  • [Presentation] ショウジョウバエの神経筋シナプス形成におけるヘパラン硫酸プロテオグリカンの機能解析2009

    • Author(s)
      神村圭亮、前田信明
    • Organizer
      第29回日本糖質学会
    • Place of Presentation
      飛騨世界生活文化センター(岐阜県)
    • Year and Date
      2009-09-10
    • Related Report
      2009 Annual Research Report
  • [Remarks]

    • URL

      http://www.igakuken.or.jp/research/project/res_prj08.html

    • Related Report
      2011 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

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