Peptidomics-based mining of novel antimicrobial peptides

• Project/Area Number: 21770156
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: National Cardiovascular Center Research Institute
• Principal Investigator: OSAKI Tsukasa 独立行政法人国立循環器病研究センター, 分子薬理部, 特任研究員 (60380565)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: ホルモンと生理活性物質 / 抗菌ペプチド / ペプチドーム / 抗生物質 / 感染症

Research Abstract

Recently, it has been an increasing problem of drug-resistant bacteria in a hospital. One of candidates effective against these bacteria is an antimicrobial peptide. This study was aimed at discovery of novel antimicrobial peptides from identified peptides using peptidomic analysis. We screened them based on antimicrobial activity and found two novel antimicrobial peptides including AMP-IBP5. AMP-IBP5 is an intramolecular disulfide-linked 22-residue amidated peptide. This peptide showed antimicrobial activity stronger than β-defensin-2 and could become candidates for new drugs.

Research Products

• [Journal Article] Peptidomics-based discovery of an antimicrobial peptide derived from insulin-like growth factor-binding protein 5. (2011)
  • Author(s): T.Osaki, K.Sasaki, N.Minamino
  • Journal Title: Journal of Proteome Research 10 Pages: 1870-1880
  • Peer Reviewed
• [Journal Article] Peptidomics-based discovery of an antimicrobial peptide derived from insulin-like growth factor-binding protein 5 (2011)
  • Author(s): 尾崎司
  • Journal Title: Journal of Proteome Research Volume: 10 Pages: 1870-1880
  • Peer Reviewed
• [Journal Article] Secretopeptidome mining for novel bioactive peptides (2009)
  • Author(s): 尾崎司
  • Journal Title: Peptide Science Pages: 143-144
  • Peer Reviewed
• [Presentation] Secretopeptidome mining for novel biologically active peptides (2010)
  • Author(s): Tsukasa Osaki, Kazuki Sasaki, Naoto Minamino
  • Organizer: 第14回国際内分泌会議ICE2010
  • Place of Presentation: 京都
  • Year and Date: 2010-03-30
• [Presentation] Secretopeptidome mining for novel biologically active peptides (2010)
  • Author(s): 尾崎司
  • Organizer: 第14回国際内分泌学会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2010-03-30
• [Presentation] Secretopeptidome mining for novel bioactive peptides (2009)
  • Author(s): 尾崎司、佐々木一樹、南野直人
  • Organizer: 第46回ペプチド討論会
  • Place of Presentation: 北九州(福岡)
  • Year and Date: 2009-11-04
• [Presentation] 分泌ペプチドーム解析による新規活性ペプチドの探索 (2009)
  • Author(s): 尾崎司
  • Organizer: 第46回ペプチド討論会
  • Place of Presentation: 北九州国際会議場
  • Year and Date: 2009-11-04
• [Book] Secretopeptidome mining for novel bioactive peptides. (2009)
  • Author(s): T.Osaki, K.Sasaki, N.Minamino
  • Publisher: Peptide Science
• [Patent(Industrial Property Rights)] ペプチド及びその用途 (2010)
  • Inventor(s): 尾崎司、佐々木一樹、南野直人、南祇利実
  • Industrial Property Rights Holder: 独立行政法人国立循環器病研究センター・総長、武田薬品工業株式会社
  • Filing Date: 2010-07-06
• [Patent(Industrial Property Rights)] ペプチドおよびその用途 (2010)
  • Inventor(s): 尾崎司
  • Industrial Property Rights Holder: 国立循環器病研究センター総長
  • Filing Date: 2010-06-15
  • Overseas
• [Patent(Industrial Property Rights)] ペプチド及びその用途 (2009)
  • Inventor(s): 尾崎司、佐々木一樹、南野直人、南祇利実
  • Industrial Property Rights Holder: 独立行政法人国立循環器病研究センター・総長、武田薬品工業株式会社
  • Industrial Property Number: 2009-142232
  • Filing Date: 2009-06-15
• [Patent(Industrial Property Rights)] ペプチドおよびその用途 (2009)
  • Inventor(s): 尾崎司
  • Industrial Property Rights Holder: 国立循環器病センター総長
  • Industrial Property Number: 2009-142232
  • Filing Date: 2009-06-15