Investigation about the behavior of supramolecular complex in cell membrane : mechanism

• Project/Area Number: 21770157
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: Tohoku University
• Principal Investigator: FUKUOKA Hajime 東北大学, 多元物質科学研究所, 助教 (50447190)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 分子モーター / イメージング / バクテリア / 一分子計測 / イオン

Research Abstract

Expression systems for GFP-FliN, FliM-GFP, and GFP-FliG were constructed, and the functional incorporation was confirmed. In a fluorescently labeled motor by GFP, the recovery of fluorescence after photobleaching by the evanescent light was observed. This is the first evidence for the exchange of rotor components in a functioning bacterial flagellar motor. The rotation of motors of individual E. coli cells by combining a high-speed camera and the bead assay method. From this experiment, it was showed that the switching of two different motors on a cell is controlled coordinatedly by an intracellular signal protein, phosphorylated CheY. From these results, wave-like propagation model of intracellular signal was proposed.

Research Products

• [Journal Article] Coordinated regulation of multiple flagellar motors by the Escherichia coli chemotaxis system. Biophysics (2012)
  • Author(s): Fukuoka, H., Inoue, Y., Ishijima, A.
  • Journal Title: Biophysics Volume: 8 Pages: 59-66
  • Peer Reviewed
• [Journal Article] Coordinated regulation of multiple flagellar motors by the Escherichia coli chemotaxis system (2012)
  • Author(s): Hajime Fukuoka, Yuichi Inoue, Akihiko Ishijima
  • Journal Title: BIOPHYSICS Volume: 8 Pages: 59-66
  • DOI: 10.2142/biophysics.8.59
  • NAID: 130002061593
  • ISSN: 1349-2942
  • Peer Reviewed
• [Journal Article] 大腸菌複数べん毛モーターの回転方向転換における同期性 (2011)
  • Author(s): 福岡創
  • Journal Title: 生物物理 Volume: 51 Pages: 234-235
  • NAID: 10029482520
  • Peer Reviewed
• [Journal Article] Coordinated Reversal of Flagellar Motors on a Single Escherichia coli Cell (2011)
  • Author(s): Terasawa, S., Fukuoka, H., Inoue, Y., Sagawa, T., Takahashi, H., and Ishijima, A.
  • Journal Title: Biophys. J Volume: 100 Pages: 2193-2200
  • Peer Reviewed
• [Journal Article] Coordinated Reversal of Flagellar Motors on a Single Escherichia coli Cell (2011)
  • Author(s): Shun Terasawa, Hajime Fukuoka, Yuichi Inoue, Takashi Sagawa, Hiroto Takahashi, Akihiko Ishijima
  • Journal Title: Biophysical Journal Volume: 100 Issue: 9 Pages: 2193-2200
  • DOI: 10.1016/j.bpj.2011.03.030
  • Peer Reviewed
• [Journal Article] Exchange of rotor components in functioning bacterial flagellar motor (2010)
  • Author(s): Fukuoka, H., Inoue, Y., Terasawa, S., Takahashi, H., and Ishijima, A.
  • Journal Title: Biochem. Biophys. Res. Commun Volume: 394 Pages: 130-135
  • Peer Reviewed
• [Journal Article] Exchange of rotor components in functioning bacterial flagellar motor (2010)
  • Author(s): Hajime Fukuoka, Yuichi Inoue, Shun Terasawa, Hiroto Takahashi, Akihiko Ishijima
  • Journal Title: Biochemical and Biophysical Research Communications 26 Pages: 130-135
  • Peer Reviewed
• [Presentation] Coordinated Regulation of Multiple Locomotive (2012)
  • Author(s): Fukuoka, H., Sagawa, T., Inoue, Y., Takahashi, T., Ishijima, A.
  • Organizer: apparatus in a Single Bacterial Cell. International Symposium on Innovative Nanobiodevices ISIN2012
  • Place of Presentation: 名古屋
  • Year and Date: 2012-03-21
• [Presentation] Coordinated Regulation of Multiple Locomotive apparatus in a Single Bacterial Cell (2012)
  • Author(s): Hajime Fukuoka, Takashi Sagawa, Yuichi Inoue, Hiroto Takahashi, Akihiko Ishijima
  • Organizer: International Symposium on Innovative Nanobiodevices ISIN2012
  • Place of Presentation: 日本,名古屋
  • Year and Date: 2012-03-21
• [Presentation] Role of the polar localization of CheZ for the propagation of intracellular signal in bacterial chemotaxis system (2012)
  • Author(s): Fukuoka, H.
  • Organizer: Gordon Research Conferences, Sensory Transduction in Microorganisms
  • Place of Presentation: USA, Ventura
  • Year and Date: 2012-01-17
• [Presentation] Consideration of the role of CheZ in the intracellular signaling of Escherichia coli cell (2011)
  • Author(s): Fukuoka, H., Inoue, Y., Takahashi, H., Ishijima, A.
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
  • Year and Date: 2011-09-18
• [Presentation] Consideration of the role of CheZ in the intracellular signaling of Escherichia coli cell (2011)
  • Author(s): 福岡創, 井上裕一, 高橋泰人, 石島秋彦
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 日本,姫路
  • Year and Date: 2011-09-18
• [Presentation] Coordinated regulation of flagellar motors on a single Escherichia coli cell (2011)
  • Author(s): Fukuoka, H., Terasawa, S., Inoue, Y., Sagawa, T., Takahashi, H., Ishijima, A.
  • Organizer: BLAST XI meeting
  • Place of Presentation: USA, New Orleans
  • Year and Date: 2011-01-18
• [Presentation] 偏光による蛍光分子の配向検出のための顕微鏡構築 (2010)
  • Author(s): 福岡創, 清水孝浩, 井上裕一, 高橋泰人, 石島秋彦
  • Organizer: 第36回日本生体エネルギー研究会
  • Place of Presentation: 吹田市
  • Year and Date: 2010-11-18
• [Presentation] 偏光による蛍光分子の配向検出のための顕微鏡構築 (2010)
  • Author(s): 福岡創, 清水孝浩, 井上裕一, 高橋泰人, 石島秋彦
  • Organizer: 第36回日本生体エネルギー研究会
  • Place of Presentation: 日本,吹田市
  • Year and Date: 2010-11-18
• [Presentation] Synchronous regulation of multiple flagellar motors on a single Escherichia coli cell (2010)
  • Author(s): Fukuoka, H., Terasawa, S., Inoue, Y., Takahashi, H., Ishijima, A.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-20
• [Presentation] Synchronous regulation of multiple flagellar motors on a single Escherichia coli cell (2010)
  • Author(s): Hajime Fukuoka, Shun Terasawa, Yuichi Inoue, Hiroto Takahashi, Akihiko Ishijima
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 日本,仙台
  • Year and Date: 2010-09-20
• [Presentation] he monitoring of intracelluar signaling in one bacterial cell by the measurement of multiple flagellar rotations (2010)
  • Author(s): Fukuoka, H.
  • Organizer: International Symposium of Joint Research Network on Advanced Materials and Devices
  • Place of Presentation: Hokkaido, Japan
  • Year and Date: 2010-03-25
• [Presentation] The monitoring of intracelluar signaling in one bacterial cell by the measurement of multiple flagellar rotations (2010)
  • Author(s): 福岡創
  • Organizer: International Symposium of Joint Research Network on Advanced Materials and Devices
  • Place of Presentation: Hokkaido, Japan
  • Year and Date: 2010-03-25
• [Presentation] 機能的細菌べん毛モーターにおけるモーター構成素子の交換割合の見積り (2009)
  • Author(s): 福岡創
  • Organizer: 第47回生物物理学会年
  • Place of Presentation: 徳島
  • Year and Date: 2009-10-31
• [Presentation] 機能的細菌べん毛モーターにおけるモーター構成素子の交換割合の見積り (2009)
  • Author(s): 福岡創
  • Organizer: 第47回生物物理学会年会
  • Place of Presentation: 日本, 徳島
  • Year and Date: 2009-10-31
• [Presentation] The chemotactic response and correlation of the multiple flagellar motors in a single bacterial cell (2009)
  • Author(s): Fukuoka, H.
  • Organizer: International Symposium"Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes"
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-09-08
• [Presentation] The chemotactic response and correlation of the multiple flagellar motors in a single bacterial cell (2009)
  • Author(s): 福岡創
  • Organizer: International Symposium "Innovative Nanoscience of Supermolecular Motor Proteins Working in Biomembranes"
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2009-09-08
• [Presentation] 機能的な生体回転モーターにおけるモーター構成素子交換の可視化 (2009)
  • Author(s): 福岡創
  • Organizer: 東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2009-06-16
• [Presentation] 機能的な生体回転モーターにおけるモーター構成素子交換の可視化 (2009)
  • Author(s): 福岡創
  • Organizer: 東北大学バイオサイエンスシンポジウム
  • Place of Presentation: 日本, 仙台
  • Year and Date: 2009-06-16