| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Expression systems for GFP-FliN, FliM-GFP, and GFP-FliG were constructed, and the functional incorporation was confirmed. In a fluorescently labeled motor by GFP, the recovery of fluorescence after photobleaching by the evanescent light was observed. This is the first evidence for the exchange of rotor components in a functioning bacterial flagellar motor. The rotation of motors of individual E. coli cells by combining a high-speed camera and the bead assay method. From this experiment, it was showed that the switching of two different motors on a cell is controlled coordinatedly by an intracellular signal protein, phosphorylated CheY. From these results, wave-like propagation model of intracellular signal was proposed.
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