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Nano-scale analysis and computational fluid mechanics of the flow on the surface of tracheal lumen

Research Project

Project/Area Number 21770158
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionTohoku University

Principal Investigator

UENO Hironori  東北大学, 国際高等研究教育機構, 助教 (70518240)

Research Collaborator ISHIKAWA Takuji  東北大学, 大学院・工学研究科 (20313728)
ISHIKAWA Takashi  ポールシェラ研究所
Project Period (FY) 2009 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2011: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords繊毛・鞭毛 / 気管 / ダイニン / クライオ電子線トモグラフィー法 / 数値シミュレーション / 気管上皮細胞
Research Abstract

Mucociliary clearance on the surface of the tracheal lumen is an important component of lung defense against viruses and bacteria. We analyzed the detail of ciliary movement with high spatial precision by labeling the ciliary tip using quantum dots, and axonemal structure by cryo-electron tomography. We revealed the overall directional flow from the lungs to the oropharynx on tracheal lumen by effective ciliary motion with the asymmetric axonemal structure. Moreover, we discovered that collective swimming of microorganisms is the most energy-efficient way of moving. And also, we developed new program code and GPGPU computational system to analyze the fluid flow mechanism on the surface of tracheal lumen.

Report

(4 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • 2009 Annual Research Report
  • Research Products

    (38 results)

All 2012 2011 2010 2009 Other

All Journal Article (5 results) (of which Peer Reviewed: 4 results) Presentation (28 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Mouse respiratory cilia with the asymmetric axonemal structure on sparsely distributed ciliary cells can generate overall directional flow. Nanomedicine2012

    • Author(s)
      Hironori Ueno, Takuji Ishikawa, Khanh Huy Bui, Kohsuke Gonda K, Takashi Ishikawa, Yamaguchi T.
    • Journal Title

      Nanotechnology, Biology, and Medicine

      Volume: (In press)

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mouse respiratory cilia with the asymmetric axonemal structure on sparsely distributed ciliary cells can generate overall directional flow2012

    • Author(s)
      Hironori Ueno, et al
    • Journal Title

      Nanomedicine : Nanotechnology, Biology, and Medicine

      Volume: (未定)(In press) Issue: 7 Pages: 1081-1087

    • DOI

      10.1016/j.nano.2012.01.004

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Energy Transport in a Concentrated Suspension of Bacteria2011

    • Author(s)
      Takuji Ishikawa, Naoto Yoshida, Hironori Ueno, M. Wiedeman, Yosuke Imai, and Takami Yamaguchi
    • Journal Title

      Physical Review Letters

      Volume: Vol.107, No.2

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Energy Transport in a Concentrated Suspension of Bacteria2011

    • Author(s)
      Takuji Ishikawa, et al
    • Journal Title

      Physical Review Letters

      Volume: 107 Issue: 2

    • DOI

      10.1103/physrevlett.107.028102

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] ダイニン・微小管複合体の高分解能構造解析2009

    • Author(s)
      広瀬恵子・上野裕則
    • Journal Title

      顕微鏡(特集:生体高分子の高分解能構造解析) Vol.44

      Pages: 93-97

    • NAID

      130007788907

    • Related Report
      2009 Annual Research Report
  • [Presentation] Nano-imaging of respiratory cilia with Light and electron microscopy2012

    • Author(s)
      Hironori Ueno, et al
    • Organizer
      5th International Symposium on Nanomedicine (ISNM2011)
    • Place of Presentation
      University of Nagoya
    • Year and Date
      2012-05-16
    • Related Report
      2011 Annual Research Report
  • [Presentation] マウス気管繊毛の運動とクライオ電子線トモグラフィー法による軸糸構造2012

    • Author(s)
      上野裕則, 他
    • Organizer
      第24回日本機械学会バイオエンジニアリング講演会
    • Place of Presentation
      大阪大学(招待講演)
    • Year and Date
      2012-01-07
    • Related Report
      2011 Annual Research Report
  • [Presentation] Nano-imaging of respiratory cilia with light and electron microscopy2012

    • Author(s)
      Hironori Ueno
    • Organizer
      5th International Symposium on Nanomedicine(ISNM2011)
    • Place of Presentation
      the University of Nagoya, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] マウス気管繊毛の運動とクライオ電子線トモグラフィー法による軸糸構造2012

    • Author(s)
      上野裕則
    • Organizer
      第24回日本機械学会バイオエンジニアリング講演会
    • Place of Presentation
      大阪大学
    • Related Report
      2011 Final Research Report
  • [Presentation] 大腸菌懸濁液内のエネルギー輸送2012

    • Author(s)
      石川拓司、吉田尚人、上野裕則、Wiedeman M. 、今井陽介、山口隆美
    • Organizer
      第24回日本機械学会バイオエンジニアリング講演会
    • Place of Presentation
      大阪大学
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] バクテリアの遊泳能を利用したソーティングデバイスの開発2012

    • Author(s)
      塩入達也、石川拓司、沼山恵子、今井陽介、上野裕則、山口隆美
    • Organizer
      第24回日本機械学会バイオエンジニアリング講演会
    • Place of Presentation
      大阪大学
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] マウス気管における繊毛運動・流体解析、及びクライオ電子線トモグラフィー法による軸糸構造解析2012

    • Author(s)
      上野裕則
    • Organizer
      2012年生体運動研究合同班会議
    • Place of Presentation
      筑波大学
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Analysis of fluid flow on the surface of tracheal lumen through effective ciliary motion2011

    • Author(s)
      Koki Kiyota, et al
    • Organizer
      5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
    • Place of Presentation
      National University of Singapore (NUS), Singapore
    • Year and Date
      2011-12-12
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of ciliary motion and the axonemal structure in the mouse respiratory cilia2011

    • Author(s)
      Hironori Ueno, et al
    • Organizer
      International Symposium on Mechanobiology (The Fifth Shanghai International Conference on Biophysics and Molecular biology)
    • Place of Presentation
      Shanghai, China(招待講演)
    • Year and Date
      2011-11-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Development of a micro fluidic device to separate motile bacteria2011

    • Author(s)
      Tatsuya Shioiri, et al
    • Organizer
      ASME, 6th Frontiers in Biomedical Devices Conference
    • Place of Presentation
      Irvine, CA, USA
    • Year and Date
      2011-09-26
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of ciliary motion and fluid flow on the surface of tracheal cells2011

    • Author(s)
      Hironori Ueno, et al
    • Organizer
      Japan-Portugal Nano-BME Symposium
    • Place of Presentation
      University of Porto, Portgal(招待講演)
    • Year and Date
      2011-06-06
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of ciliary motion and fluid flow on the surface of tracheal cells2011

    • Author(s)
      Hironori Ueno, et al
    • Organizer
      Workshop of Microscale Modeling in Biomechanics and Mechanobiology
    • Place of Presentation
      Hotel Vila Gale, Ericeria, Portgal(招待講演)
    • Year and Date
      2011-05-31
    • Related Report
      2011 Annual Research Report
  • [Presentation] Analysis of fluid flow on the surface of tracheal lumen through effective ciliary motion2011

    • Author(s)
      Koki Kiyota, Hironori Ueno, Takuji Ishikawa, Keiko Numayama-Tsuruta, Yosuke Imai, Noriaki Matsuki and Takami Yamaguchi
    • Organizer
      5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering, Singapore
    • Place of Presentation
      National University of Singapore(NUS)
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of a micro fluidic device to separate motile bacteria2011

    • Author(s)
      Tatsuya Shioiri, Takuji Ishikawa, Keiko Numayama-Tsuruta, Hironori Ueno, Yosuke Imai and Takami Yamaguchi
    • Organizer
      5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
    • Place of Presentation
      National University of Singapore (NUS), Singapore
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Entrapment of fresh water ciliates at the interface fluid-air2011

    • Author(s)
      Jonathan Ferracci, Takuji Ishikawa, Hironori Ueno, Keiko Numayama-Tsuruta, Yosuke Imai and Takami Yamaguchi
    • Organizer
      5th East Asian Pacific Student Workshop on Nano-Biomedical Engineering
    • Place of Presentation
      National University of Singapore (NUS), Singapore
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Analysis of ciliary motion and the axonemal structure in the mouse respiratory cilia2011

    • Author(s)
      Hironori Ueno
    • Organizer
      International Symposium on Mechanobiology(The Fifth Shanghai International Conference on Biophysics and Molecular biology)
    • Place of Presentation
      Shanghai-Hangzhou, China
    • Related Report
      2011 Final Research Report
  • [Presentation] Development of a microdevice for sorting motile bacteria2011

    • Author(s)
      Tatsuya Shioiri, Takuji Ishikawa, Keiko Numayama-Tsuruta, Hironori Ueno, Yosuke Imai and Takami Yamaguchi
    • Organizer
      ASME, 6th Frontiers in Biomedical Devices Conference
    • Place of Presentation
      Irvine, CA, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Analysis of ciliary motion and fluid flow on the surface of tracheal cells2011

    • Author(s)
      Hironori Ueno
    • Organizer
      Workshop of Microscale Modeling in Biomechanics and Mechanobiology
    • Place of Presentation
      Hotell Vila Gale, ericeria, Portgal
    • Related Report
      2011 Final Research Report
  • [Presentation] Analysis of ciliary motion and fluid flow on the surface of tracheal cells2011

    • Author(s)
      Hironori Ueno
    • Organizer
      Japan-Portugal Nano-BME Symposium, Faculty of Engineering of the University of Porto
    • Place of Presentation
      Polytechnic Institute of Braganca
    • Related Report
      2011 Final Research Report
  • [Presentation] Dynein generates force by winch mechanism2010

    • Author(s)
      Hironori Ueno
    • Organizer
      ESF-EMBO, (Emergent Propert is of the Cytoskelton : Molecules to Cells)
    • Place of Presentation
      Hotel Eden Roc, Sant Feliu de Guixols, Spain
    • Year and Date
      2010-10-05
    • Related Report
      2010 Annual Research Report
  • [Presentation] 大腸菌を用いたバイオマイクロミキサーの基礎研究2010

    • Author(s)
      松浦亮、石川拓司、上野裕則、今井陽介、松木範明、山口隆美
    • Organizer
      第45回総会・講演会日本機械学会東北支部会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-03-12
    • Related Report
      2011 Final Research Report
  • [Presentation] 共焦点マイクロPIVシステムによる大腸菌溶液のメゾスケール流動構造の計測2010

    • Author(s)
      吉田尚人、石川拓司、上野裕則、今井陽介、松木範明、山口隆美
    • Organizer
      第45回総会・講演会日本機械学会東北支部会
    • Place of Presentation
      東北大学
    • Year and Date
      2010-03-12
    • Related Report
      2011 Final Research Report
  • [Presentation] Dynein generates force by winch mechanism2010

    • Author(s)
      Hironori Ueno
    • Organizer
      ESF-EMBO,(Emergent Propertis of the Cytoskelton : Molecules to Cells)
    • Place of Presentation
      Hotel Eden Roc, Sant Feliu de Guixols, Spain
    • Related Report
      2011 Final Research Report
  • [Presentation] FLUID FLOW ANALYSIS ON THE SURFACE OF TRACHEAL CILIARY CELLS2009

    • Author(s)
      Hironori Ueno
    • Organizer
      Dyenin 2009
    • Place of Presentation
      神戸
    • Year and Date
      2009-11-03
    • Related Report
      2009 Annual Research Report
  • [Presentation] Structural changes in dynein molecules accompanying force generation2009

    • Author(s)
      Hironori Ueno
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      徳島
    • Year and Date
      2009-10-31
    • Related Report
      2009 Annual Research Report
  • [Presentation] Structural changes in dynein molecules accompanying force generation2009

    • Author(s)
      Hironori Ueno, Takuo Yasunaga, Chikako Shingyoji, Takami Yamaguchi and Keiko Hirose
    • Place of Presentation
      アスティ徳島(徳島)
    • Related Report
      2011 Final Research Report
  • [Presentation] Fluid flow analysis on the surface of tracheal ciliary cells2009

    • Author(s)
      Hironori Ueno
    • Organizer
      International Dynein Workshop 2009
    • Place of Presentation
      神戸ファッション美術館オルビスホール
    • Related Report
      2011 Final Research Report
  • [Presentation] Dynein pulls microtubules without rotating its stalk2009

    • Author(s)
      Hironori Ueno
    • Organizer
      ASME Summer Bioengineering Conference
    • Place of Presentation
      Lake Tahoe, CA, USA
    • Related Report
      2011 Final Research Report
  • [Book] ダイニン・微小管複合体の高分解能構造解析顕微鏡(特集:生体高分子の高分解能構造解析)2009

    • Author(s)
      広瀬恵子、上野裕則
    • Related Report
      2011 Final Research Report
  • [Remarks] 研究室HP、プレスリリース関連

    • URL

      http://www.pfsl.mech.tohoku.ac.jp/contents/press_ueno/ueno_press_jp.html

    • Related Report
      2011 Final Research Report
  • [Remarks] 東北大学HP、プレスリリース

    • URL

      http://www.tohoku.ac.jp/japanese/2012/02/press20120213-03.html

    • Related Report
      2011 Final Research Report
  • [Remarks] 研究室HP

    • URL

      http://www.pfsl.mech.tohoku.ac.jp/contents/press_ueno/ueno_press_jp.html

    • Related Report
      2011 Annual Research Report
  • [Remarks] 東北大学プレスリリース

    • URL

      http://www.tohoku.ac.jp/japanese/2012/02/press20120213-03.html

    • Related Report
      2011 Annual Research Report

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Published: 2009-04-01   Modified: 2016-04-21  

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