Neutron crystallographic analysis of human transthyretin

• Project/Area Number: 21770159
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: University of Toyama (2011); Ibaraki University (2009-2010)
• Principal Investigator: YOKOYAMA Takeshi 富山大学, 大学院・医学薬学研究部(薬学), 助教 (50524162)
• Project Period (FY): 2009 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 中性子結晶構造解析 / 構造生物学 / アミロイドーシス

Research Abstract

Towards to the neutron crytallographic analysis, crystallization of large crytals and neutron diffraction data collection were carried out, and we succeeded to determine the neutron crystal structure of transthyretin. Neutron structure revealed the structural stability and molecular pH sensitivity, related to the amyloid fibril formation.

Research Products

• [Journal Article] Hydrogen-bond network and pH sensitivity in transthyretin : Neutron crystal structure of human transthyretin (2012)
  • Author(s): Yokoyama T., Mizuguchi M., Nabeshima Y., Kusaka K., Yamada T., Hosoya T., Ohhara T., Kurihara K., Tomoyori K., Tanaka I., Niimura N.
  • Journal Title: J. Struct. Biol Volume: 177-2 Pages: 283-290
  • Peer Reviewed
• [Journal Article] Hydrogen-bond network and pH sensitivity in transthyretin : neutron crystal structure of human transthyretin (2012)
  • Author(s): Yokoyama, T., Mizuguchi, M., 他9名
  • Journal Title: Journal of Structural Biology Volume: 177 Issue: 2 Pages: 283-290
  • DOI: 10.1016/j.jsb.2011.12.022
  • Peer Reviewed
• [Journal Article] Neutron structure analysis using the IBARAKI biological crystal diffractometer(iBIX) at J-PARC (2010)
  • Author(s): Tanaka I., Kusaka K., Hosoya T., Niimura N., Ohhara T., Kurihara K., Yamada T., Ohnishi Y., Tomoyori K., Yokoyama T.
  • Journal Title: Acta Crystallogr D Biol Crystallogr Volume: 66-11 Pages: 1194-1197
  • Peer Reviewed
• [Journal Article] Structure and function of a Campylobacter jejuni thioesterase Cj0915, a hexameric hot dog fold enzyme (2009)
  • Author(s): Yokoyama T., Choi KJ., Bosch AM., Yeo HJ.
  • Journal Title: Biochim Biophys Acta Volume: 1974-7 Pages: 1073-1081
  • Peer Reviewed
• [Journal Article] Structure of a sigma28-regulated nonflagellar virulence protein from Campylobacter jejuni (2009)
  • Author(s): Kirkpatrick AS., Yokoyama T., Choi KJ., Yeo HJ.
  • Journal Title: Biochem Biophys Res Commun Volume: 6 ; 380(2) Pages: 407-412
  • Peer Reviewed
• [Presentation] トランスサイレチンの結晶構造解析 (2012)
  • Author(s): 横山武司
  • Organizer: 第三回MLFシンポジウム
  • Place of Presentation: 東海村
  • Year and Date: 2012-01-19
• [Presentation] トランスサイレチンの結晶構造解析 (2012)
  • Author(s): 横山武司
  • Organizer: 第3回MLFシンポジウム
  • Place of Presentation: 東海(口頭発表)
• [Presentation] トランスサイレチンの結晶構造解析 (2011)
  • Author(s): 横山武司
  • Organizer: 平成22年度茨城県中性子ビームライン成果報告会
  • Place of Presentation: 東京(口頭発表)
  • Year and Date: 2011-09-26
• [Presentation] トランスサイレチンの結晶構造解析 (2011)
  • Author(s): 横山武司
  • Organizer: 平成22年度茨城県中性子ビームライン成果報告会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-26
• [Presentation] トランスサイレチンの中性子結晶構造解析 (2011)
  • Author(s): 横山武司
  • Organizer: 第12回Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 富山(口頭発表)
  • Year and Date: 2011-06-18
• [Presentation] トランスサイレチンの中性子結晶構造解析 (2011)
  • Author(s): 横山武司
  • Organizer: 第12回Pharmaco-Hematologyシンポジウム
  • Place of Presentation: 富山
  • Year and Date: 2011-06-18
• [Presentation] XXII Congress and General Assembly International Union of Crystallography(IUCr2011) (2011)
  • Author(s): Yokoyama T., Nabeshima Y., Hosoya T., Ohhara T., Kurihara K., Kusaka K., Mizuguchi M., Tanaka I., Niimura N.
  • Place of Presentation: マドリッド(ポスター発表)
• [Presentation] Preliminary neutron crystallographic study of mutant transthyretin (2011)
  • Author(s): Takeshi Yokoyama
  • Organizer: XXII Congress and General Assembly International Union of Crystallography
  • Place of Presentation: マドリッド(スペイン)
• [Presentation] Preliminary neutron crystallographic study and X-ray structural analysis of mutant Transthyretin (2010)
  • Author(s): Takeshi Yokoyama
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大学・川内北キャンパス
  • Year and Date: 2010-09-21
• [Presentation] 変異体トランスサイレチンの中性子回折測定及びX線結晶構造解析 (2010)
  • Author(s): Yokoyama T., Nabeshima Y., Hosoya T., Ohhara T., Kurihara K., Kusaka K., Mizuguchi M., Tanaka I., Niimura N.
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 仙台(ポスター発表)
  • Year and Date: 2010-09-20
• [Presentation] 変異体トランスサイレチンの中性子回折実験 (2010)
  • Author(s): 横山武司
  • Organizer: 水和ナノ構造研究会
  • Place of Presentation: 伊豆(口頭発表)
  • Year and Date: 2010-09-06
• [Presentation] Towards the neutron crystallographic analysis of amyloid protein, TTR. (2009)
  • Author(s): Yokoyama T., Nabeshima Y., Mizuguchi M., Tanaka I., Niimura N.
  • Organizer: 第47回日本生物物理学会年会
  • Place of Presentation: 徳島(ポスター発表)
  • Year and Date: 2009-10-30
• [Presentation] アミロイドーシス原因タンパク質トランスサイレチンの中性子結晶構造解析に向けて (2009)
  • Author(s): 横山武司
  • Organizer: 日本生物物理学会 第47回
  • Place of Presentation: 徳島・アスティとくしま
• [Remarks]
  • URL: http://info.ibaraki.ac.jp/scripts/websearch/index.htm