• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of 2-step excitation transient grating method, and elucidation of photo-reaction mechanism and control of signal transferring protein.

Research Project

Project/Area Number 21770165
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Biophysics
Research InstitutionNagoya Institute of Technology

Principal Investigator

INOUE Keiichi  Nagoya Institute of Technology, 大学院・工学研究科, 助教 (90467001)

Research Collaborator SUDO Yuki  名古屋大学, 理学研究科, 准教授 (10452202)
Project Period (FY) 2009 – 2010
Project Status Completed (Fiscal Year 2010)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2010: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2009: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Keywords光受容タンパク質 / 過渡回折格子法 / 光受容蛋白質
Research Abstract

Sensory rhodopsin I from eubacterium Salinibacter ruber is the photo-censor for the bacterial photo-taxis. In this study the photo-reaction dynamics of this protein was studied by transient grating (TG) method. As the result, the presences of new photo-intermediates were identified in the photo-cycle of sensory rhodopsin I. In addition, the enthalpy differences of the intermediates were determined by TG method and we found that they are significantly affected by the binding and dissociation of chloride ion.

Report

(3 results)
  • 2010 Annual Research Report   Final Research Report ( PDF )
  • 2009 Annual Research Report
  • Research Products

    (44 results)

All 2011 2010 2009 Other

All Journal Article (15 results) (of which Peer Reviewed: 15 results) Presentation (28 results) Remarks (1 results)

  • [Journal Article] Spectrally Silent Intermediates during the Photochemical Reactions of Salinibacter Sensory Rhodopsin I2011

    • Author(s)
      Keiichi Inoue, Yuki Sudo, Michio Homma, Hideki Kandori
    • Journal Title

      The Journal of Physical Chemistry B

      Volume: 115 Pages: 4500-4508

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Spectrally Silent Intermediates during the Photochemical Reactions of Salinibacter Sensory Rhodopsin I2010

    • Author(s)
      Keiichi Inoue、Yuki Sudo、Michi Homma、Hideki Kandori
    • Journal Title

      J.Phys.Chem.B 112巻

      Pages: 2542-2547

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Chimeric Microbial Rhodopsins Containing the Third Cytoplasmic Loop of Bovine Rhodopsin2010

    • Author(s)
      Aya Nakatsuma、Takahiro Yamashita、Kengo Sasaki、Akira Kawanabe、Keiichi Inoue、Yuji Furutani、Yoshinori Shichida、Hideki Kandori
    • Journal Title

      Biophysical.Journal 100

      Pages: 1874-1882

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dual Emission Caused by Ring Inversion Isomerization of a 4-Methyl-2-pyridyl-pyrimidine Copper(I) Complex2010

    • Author(s)
      Michihiro Nishikawa、Kuniharu Nomoto、Shoko Kume、Keiichi Inoue、Makoto Sakai、Masaaki Fujii、Hiroshi Nishihara
    • Journal Title

      J.Am.Chem.Soc. 132

      Pages: 9579-9581

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Infrared Imaging of an A549 Cultured Cell by a Vibrational Sum-frequency Generation Detected Infrared Super-resolution Microscope2010

    • Author(s)
      Satoshi Kogure、Keiichi Inoue、Tsutomu Ohmori、Miya Ishihara、Makoto Kikuchi、Masaaki Fujii、Makoto Sakai
    • Journal Title

      Optics Express 18

      Pages: 13402-13406

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] 蛍光検出を利用した赤外超解像顕微鏡法によるA549細胞の測定(Infrared Super-Resolution Imaging of a Single A549 Cell by Fluorescence Detection)2010

    • Author(s)
      大森努、井上圭一、酒井誠、藤井正明、石原美弥、菊地眞
    • Journal Title

      日本レーザー医学会誌 30

      Pages: 435-440

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development of a Non-scanning VSFG detected IR Super-resolution Microscope and its Application to Biological Cells2010

    • Author(s)
      Keiichi Inoue、Masaaki Fujii、Makoto Sakai、
    • Journal Title

      Applied Spectroscopy 64

      Pages: 275-281

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Spectroscopic studies of a sensory rhodopsin I homologue from the archaeon Haloarcula vallismortis2010

    • Author(s)
      Jin Yagasaki、Daisuke Suzuki、Kunio Ihara、Keiichi Inoue、Takashi Kikukawa、Makoto Sakai、Masaaki Fujii、Michio Homma、Hideki Kandori、Yuki Sudo
    • Journal Title

      Biochemistry 49

      Pages: 1183-1190

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Visible-super-resolution Infrared Microscopy Using Saturated Transient Fluorescence Detected Infrared Spectroscopy2010

    • Author(s)
      Nandor Bokor、Keiichi Inoue、Satoshi Kogure、Masaaki Fujii、Makoto Sakai
    • Journal Title

      Optics Communications 283

      Pages: 509-514

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a Signaling Complex Composed of Sensory Rhodopsin I and Its Cognate Transducer Protein from the Eubacterium Salinibacter ruber2009

    • Author(s)
      Yuki Sudo、Akiko Okada、Daisuke Suzuki、Keiichi Inoue、Hiroki Irieda、Makoto Sakai、Masaaki Fujii、Yuji Furutani、Hideki Kandori、Michio Homma
    • Journal Title

      Biochemistry 48

      Pages: 10136-10145

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two-point-separation in a Sub-micron Nonscanning IR Super-resolution Microscope Based on Transient Fluorescence Detected IR Spectroscopy2009

    • Author(s)
      Keiichi Inoue、Nandor Bokor、Satoshi Kogure、Masaaki Fujii、Makoto Sakai
    • Journal Title

      Virtual Journal for Biomedical Optics 4

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Two-point-separation in a Sub-micron Nonscanning IR Super-resolution Microscope Based on Transient Fluorescence Detected IR Spectroscopy2009

    • Author(s)
      Keiichi Inoue、Nandor Bokor、Satoshi Kogure、Masaaki Fujii、Makoto Sakai
    • Journal Title

      Optics Express 17

      Pages: 12013-12018

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Chloride Ion Binding on the Photochemical Properties of Salinibacter Sensory Rhodopsin I2009

    • Author(s)
      Daisuke Suzuki、Yuji Furutani、Keiichi Inoue、Takashi Kikukawa、Makoto Sakai、Masaaki Fujii、Hideki Kandori、Michio Homma、Yuki Sudo
    • Journal Title

      Journal of Molecular Biology 392

      Pages: 48-62

    • Related Report
      2010 Final Research Report
    • Peer Reviewed
  • [Journal Article] Characterization of a Signaling Complex Composed of Sensory Rhodopsin I and Its Cognate Transducer Protein from the Eubacterium Salinibacter ruber2009

    • Author(s)
      Yuki Sudo^*, Akiko Okada, Daisuke Suzuki, Keiichi Inoue, Hiroki Irieda, Makoto Sakai, Masaaki Fujii, Yuji Furutani, Hideki Kandori, Michio Homma
    • Journal Title

      Biochemistry 48

      Pages: 10136-10145

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of Chloride Ion Binding on the Photochemical Properties of Salinibacter Sensory Rhodopsin I2009

    • Author(s)
      Daisuke Suzuki, Yuji Furutani, Keiichi Inoue, Takashi Kikukawa, Makoto Sakai, Masaaki Fujii, Hideki Kandori, Michio Homma, Yuki Sudo
    • Journal Title

      Journal of Molecular Biology 392

      Pages: 48-62

    • Related Report
      2009 Annual Research Report
    • Peer Reviewed
  • [Presentation] 全反射赤外分光によるSalinibacterセンサリーロドプシンIの塩化物イオン結合サイトの構造研究2011

    • Author(s)
      Keiichi Inoue, Yuki Sudo, Michio Homma, Hideki Kandori,
    • Organizer
      日本化学会第91春季年会
    • Place of Presentation
      神奈川
    • Year and Date
      2011-03-28
    • Related Report
      2010 Final Research Report
  • [Presentation] 分光学的手法による生体π空間の制御機構解明と新機能の開拓-過渡回折格子法を用いたダイナミクス研究2011

    • Author(s)
      井上圭一、神取秀樹
    • Organizer
      新学術領域研究「高次π空間の創発と機能開発」第5回公開シンポジウム
    • Place of Presentation
      吹田
    • Year and Date
      2011-03-22
    • Related Report
      2010 Final Research Report
  • [Presentation] IR imaging of living cells by a vibrational sum-frequency generation detected IR super-resolution microscope2010

    • Author(s)
      Keiichi Inoue, Satoshi Kogure, Katsuya Kikuchi, Masaaki Fujii, Makoto Sakai,
    • Organizer
      The 2010 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2010-12-19
    • Related Report
      2010 Final Research Report
  • [Presentation] Study on the photo-reaction cycle of Salinibacter ruber Sensory rhodopsin I2010

    • Author(s)
      Keiichi Inoue, Yuki Sudo, Michio Homma, Hideki Kandori
    • Organizer
      The 2010 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Year and Date
      2010-12-18
    • Related Report
      2010 Final Research Report
  • [Presentation] Study on the photo-reaction cycle of Salinibacter ruber Sensory rhodopsin I2010

    • Author(s)
      Keiichi Inoue, Yuki Sudo, Michio Homma Hideki Kandori
    • Organizer
      PACHIFICHEM 2010
    • Place of Presentation
      Honolulu, USA
    • Year and Date
      2010-12-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] 特定領域研究「高次系分子科学」時間分解分光法によるミクロからマクロにおよぶタンパク質の反応ダイナミクスの理解2010

    • Author(s)
      井上圭一
    • Organizer
      第4回公開シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2010-11-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 時間分解分光法によるミクロからマクロにおよぶタンパク質の反応ダイナミクスの理解2010

    • Author(s)
      井上圭一
    • Organizer
      特定領域研究「高次系分子科学」第4回公開シンポジウム
    • Place of Presentation
      東北大学・片平さくらホール,仙台
    • Year and Date
      2010-11-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] 見えないものを見る分光学2010

    • Author(s)
      井上圭一
    • Organizer
      名古屋工業大学第1回化学公開セミナー
    • Place of Presentation
      名古屋
    • Year and Date
      2010-11-10
    • Related Report
      2010 Final Research Report
  • [Presentation] Dynamic conformational change of sensory rhodopsin II and transducer studied by transient grating method2010

    • Author(s)
      Keiichi Inoue
    • Organizer
      International Conference on Recent Frontiers in Applied Spectroscopy (ICORFAS-2010)
    • Place of Presentation
      Tamil Nadu, India
    • Year and Date
      2010-09-23
    • Related Report
      2010 Final Research Report
  • [Presentation] Dynamic conformational change of sensory rhodopsin II and transducer studied by transient grating method2010

    • Author(s)
      Keiichi Inoue
    • Organizer
      International Conference on Recent Frontiers in Applied Spectroscopy (ICORFAS-2010)
    • Place of Presentation
      Annamalai University, Tamil Nadu, India
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] ウシロドプシンの細胞質側第3ループを含む光駆動プロトンポンプの性質2010

    • Author(s)
      佐々木賢吾、山下高廣、中妻亜弥、井上圭一、七田芳則、神取秀樹
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-21
    • Related Report
      2010 Final Research Report
  • [Presentation] 過渡回折格子法によるbacteriorhodopsinの光反応ダイナミクスの研究2010

    • Author(s)
      井上圭一、寺嶋正秀、神取秀樹
    • Organizer
      第48回日本生物物理学会年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-20
    • Related Report
      2010 Final Research Report
  • [Presentation] Study on the photo reaction dynamics of bacteriorhodopsin by transient grating method2010

    • Author(s)
      Keiichi Inoue, Masahide Terazima, Hideki Kandori
    • Organizer
      The 48th Annual Meeting of the Biophysical Society of Japan
    • Place of Presentation
      東北大学・川内キャンパス,仙台
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report
  • [Presentation] ロドプシン/古細菌型ロドプシンキメラタンパク質を用いたGタンパク質活性化機構の解明2010

    • Author(s)
      井上圭一、佐々木賢吾、中妻亜弥、山下高廣、七田芳則、神取秀樹
    • Organizer
      第4回分子科学討論会
    • Place of Presentation
      大阪
    • Year and Date
      2010-09-15
    • Related Report
      2010 Final Research Report
  • [Presentation] ロドプシン/古細菌型ロドプシンキメラタンパク質を用いたGタンパク質活性化機構の解明2010

    • Author(s)
      井上圭一, 佐々木賢吾, 中妻亜弥, 山下高廣, 七田義則, 神取秀樹
    • Organizer
      第4回分子科学討論会
    • Place of Presentation
      大阪大学・豊中キャンパス,吹田
    • Year and Date
      2010-09-15
    • Related Report
      2010 Annual Research Report
  • [Presentation] 分子拡散過程で観るアナベナセンサリーロドプシンの蛋白質間相互作用ダイナミクス2010

    • Author(s)
      近藤正人、井上圭一、佐々木純、John L.Spudich、寺嶋正秀
    • Organizer
      2010年光化学討論会
    • Place of Presentation
      千葉
    • Year and Date
      2010-09-08
    • Related Report
      2010 Final Research Report
  • [Presentation] Transient grating studies on photo-reaction dynamics of archeal rhodopsin2010

    • Author(s)
      Keiichi Inoue
    • Organizer
      Prof.Mathies Laboratory in University of California Berkeley
    • Place of Presentation
      Berkeley, USA
    • Year and Date
      2010-08-06
    • Related Report
      2010 Final Research Report
  • [Presentation] Spectrally Silent Process and Conformational Change on Activated State of Salinibacter Sensory Rhodopsin I2010

    • Author(s)
      Satoshi Kogure, Keiichi Inoue, Katsuya Kikuchi, Kana Kitatsugu, Masaaki Fujii, Makoto Sakai,
    • Organizer
      14th International Conference on Retinal Proteins
    • Place of Presentation
      Santa Cruz, California, USA
    • Year and Date
      2010-08-06
    • Related Report
      2010 Final Research Report
  • [Presentation] Spectrally silent process and conformational change on activated state of Salinibacter sensory rhodopsin I2010

    • Author(s)
      Keiichi Inoue, Yuki Sudo, Michio Homma, Hideki Kandori
    • Organizer
      The 14th International Conference on Retinal Proteins
    • Place of Presentation
      University of California, Santa Cruz, USA
    • Year and Date
      2010-08-06
    • Related Report
      2010 Annual Research Report
  • [Presentation] 時間分解分光法によるミクロからマクロにおよぶタンパク質の反応ダイナミクスの理解2010

    • Author(s)
      井上圭一
    • Organizer
      特定領域研究「高次系分子科学」第5回班会議
    • Place of Presentation
      淡路
    • Year and Date
      2010-05-26
    • Related Report
      2010 Final Research Report
  • [Presentation] 時間分解分光法によるミクロからマクロにおよぶタンパク質の反応ダイナミクスの理解2010

    • Author(s)
      井上圭一
    • Organizer
      特定領域研究「高次系分子科学」第5回班会議
    • Place of Presentation
      南淡路ロイヤルホテル,淡路
    • Year and Date
      2010-05-26
    • Related Report
      2010 Annual Research Report
  • [Presentation] Development of a vibrational sum-frequency generation detected IR super-resolution microscope and its application to cells2010

    • Author(s)
      井上圭一, 小暮聡, 菊地克也, 藤井正明, 酒井誠
    • Organizer
      The December, 18, 2010 International Chemical Congress of Pacific Basin Societies (PACIFICHEM 2010)
    • Place of Presentation
      Honolulu, Hawaii, USA
    • Related Report
      2010 Final Research Report
  • [Presentation] 2波長レーザー分光法を用いた赤外超解像顕微鏡の開発と細胞の赤外分光測定2009

    • Author(s)
      井上圭一
    • Organizer
      日本分光学会先端レーザー分光部会 第5回先端的レーザー分光の若手シンポジウム
    • Place of Presentation
      横浜
    • Year and Date
      2009-12-11
    • Related Report
      2010 Final Research Report
  • [Presentation] HAMPドメインの構造変化ダイナミクス:センサリーロドプシンII-トランスデューサータンパク質2009

    • Author(s)
      井上圭一、寺嶋正秀
    • Organizer
      第47回日本生物物理学会年会 シンポジウム「膜蛋白質の立体構造ダイナミクス解析の最前線」
    • Place of Presentation
      徳島
    • Year and Date
      2009-11-01
    • Related Report
      2010 Final Research Report
  • [Presentation] 振動和周波発生赤外超解像顕微鏡の開発と細胞への応用2009

    • Author(s)
      井上圭一、小暮聡、菊地克也、藤井正明、酒井誠
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      徳島
    • Year and Date
      2009-10-30
    • Related Report
      2010 Final Research Report
  • [Presentation] VSFG検出赤外超解像顕微鏡を用いた生きた細胞の赤外イメージング2009

    • Author(s)
      小暮聡、井上圭一、菊地克也、藤井正明、酒井誠
    • Organizer
      第47回日本生物物理学会年会
    • Place of Presentation
      徳島
    • Year and Date
      2009-10-30
    • Related Report
      2010 Final Research Report
  • [Presentation] 振動和周波(VSFG)赤外超解像顕微鏡の開発と細胞内構造の赤外分光イメージング2009

    • Author(s)
      井上圭一、小暮聡、菊地克也、藤井正明、酒井誠
    • Organizer
      第3回分子科学討論会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-24
    • Related Report
      2010 Final Research Report
  • [Presentation] 振動和周波赤外超解像顕微鏡を用いた生細胞の赤外分光イメージング2009

    • Author(s)
      小暮聡, 井上圭一、菊地克也、藤井正明、酒井誠
    • Organizer
      第3回分子科学討論会
    • Place of Presentation
      名古屋
    • Year and Date
      2009-09-22
    • Related Report
      2010 Final Research Report
  • [Remarks] ホームページ等

    • URL

      http://www.ach.nitech.ac.jp/~physchem/kandori/index_j.html

    • Related Report
      2010 Final Research Report

URL: 

Published: 2009-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi