| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Research Abstract |
Motile microorganisms, such as bacteria and archaea, sense and respond to extracellular stimuli by changing their swimming mode to migrate towards more favorite habitats. In this research, we analyzed interaction, ion transport and structural changes in a stator complex of the bacterial flagellar motor using biophysical techniques. By means of attenuated total reflection Fourier-transform infrared spectroscopy, we directly observed binding of Na^+ to carboxylates in the Pom/B complex, including the functionally essential residue Asp24 (Sudo et al.2009b, Biochemistry). We also demonstrated that Ala39-MotB and Cys31-PomB form part of the ion flux pathway (Sudo et al. 2009, Biophysics), and reported the crystal structure of C-terminal region of MotB (Kojima et al. 2009, Mol.Microbiol.). Moreover we found and characterized new receptor proteins (Suzuki et al. 2009, J.Mol.Biol., Sudo et al.2009a, Biochemistry, Yagasaki et al.2010, Biochemistry, Sudo et al. 2011, J.Biol.Chem.).
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