Physical measurement of cell membrane by using novel optical microscopy
| Project/Area Number |
21770170
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
WATANABE Tomonobu Osaka University, 免疫学フロンティア研究センター, 特任助教 (00375205)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Keywords | 生物物理 / ナノバイオ / フォトニック結晶 / 可視化 / 1分子計測 |
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| Research Abstract |
In order to reveal mechanism of filopodia protrusion, we have developed a novel microscopy to detect nano structures like cekk membrane in real time with high contrast. Unfortunately, this development was not achieved for 2 year, however, done after the grant. Since the present developments have some trouble as above mentioned, we developed biological assay system to quantify the dynamics of myosin-X, which is responsible for filopodia protrusion (J Biol Chem., 285, 19605). Furthermore, we developed superresolution microscopy to observe the cell membrane with 90 nm and 80 ms spatial-temporal resolutions (Biophys.J., 99, L50-52).
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Report
(3 results)
Research Products
(12 results)
