Analysis of proton permeation mechanism of voltage-gated proton channel
| Project/Area Number |
21770171
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
KUROKAWA Tatsuki 大阪大学, 大学院・医学系研究科, 特任研究員 (40527701)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 生体膜 / 受容体 / チャネル / イオンチャネル / プロトン |
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| Research Abstract |
We performed site-directed cysteine scanning using accessibility of maleimide-reagent (AMS or NEM) as detected by Western blotting. When we examined the accessibility of the S4 by using NEM, we found only 6 inaccessible residues. These results suggest that the S4 segment is lined by water-accessible crevices interrupted by a small hydrophobic region.
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Report
(3 results)
Research Products
(20 results)
