| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Research Abstract |
To understand molecular mechanisms of transcriptional regulation via histone modifications, I have tried to identify the molecule(s) that is required for transcription from the chromatin template and have been focusing histone acetylation to analyze the relationship between histone modifications and transcriptional regulation. Using a purified in vitro transcription system, multiple chromatin transcription-enabling activities were identified in HeLa nuclear extract-derived fractions. Also, some of the acetylation-deficient mutants of histone H3 or H4 showed different transcription activity from the wild-type histone, suggesting that acetylation of the specific sites is important for transcriptional regulation.
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