| Project/Area Number |
21770188
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
OHKAWA Yasuyuki Kyushu University, 医学研究院, 特別准教授 (80448430)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | クロマチン / クロマチンリモデリング / ジーンクラスタリング / 細胞分化 / 高次クロマチン構造 / 骨格筋分化 / Brg1 / RNA factory |
|---|
| Research Abstract |
During skeletal muscle differentiation, the myogenic determinant MyoD binds to target genes upon its induction, promoting activation of genes expressed early in the differentiation process through recruitment of SWI/SNF ATP-dependent chromatin remodeling enzymes. The molecular basis by which late expressed genes remain transcriptionally silent during the early stages of differentiation is not well understood. We show that the regulatory sequences, but not other regions of genes highly expressed at late times of myogenesis, are in close physical proximity in differentiating embryonic tissue as well as in differentiating culture cells, despite these genes being located on different chromosomes. Global analysis of interchromosomal interactions reveals frequent genome-wide associations specifically between genes that are highly expressed in skeletal muscle but only infrequent interactions between housekeeping genes or between the two different classes of genes.
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