Mechanisms of the piRNA biogenesis in germ line cells

• Project/Area Number: 21770191
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: Keio University
• Principal Investigator: SAITO Kuniaki Keio University, 医学部, 講師 (30423396)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 生殖 / 生合成 / piRNA / ショウジョウバエ / Piwi / レトロトランスポゾン / 卵巣 / 生殖細胞 / RNAi / 培養細胞 / 3'UTR

Research Abstract

piRNAs associating with Piwi family proteins are essential small RNAs for germ cell development. In this study, I investigated how piRNAs are produced in germ cells using Drosophila melanogaster as a model organism. As a result, I have identified novel piRNA biogenesis factors and characterized the molecular functions of them. Thus, this study will contribute to our understanding of the characters of germ line cells.

Research Products

• [Journal Article] Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. (2010)
  • Author(s): Saito K., et al.
  • Journal Title: Genes & Dev. 24 Pages: 2493-2498
  • Peer Reviewed
• [Journal Article] Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila. (2010)
  • Author(s): Kuniaki Saito
  • Journal Title: Genes & Development Volume: 24 Pages: 2493-2498
  • Peer Reviewed
• [Journal Article] A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. (2009)
  • Author(s): Saito K., et al.
  • Journal Title: Nature 461 Pages: 1296-1299
  • NAID: 40019474458
  • Peer Reviewed
• [Journal Article] A regulatory circuit for piwi by the large Maf gene traffic jam in Drosophila. (2009)
  • Author(s): Kuniaki Saito
  • Journal Title: Nature 461 Pages: 1296-1299
  • NAID: 40019474458
  • Peer Reviewed
• [Presentation] Yb bodyはショウジョウバエPrimary piRNAの生合成とPiwiの核移行を制御する (2010)
  • Author(s): 齋藤都暁
  • Organizer: 日本RNA学会
  • Place of Presentation: 一橋記念講堂(東京都)
  • Year and Date: 2010-07-27
• [Presentation] New components of the primary processing pathway for Piwi-interacting piRNAs in Drosophila. (2010)
  • Author(s): Saito K.
  • Organizer: Keystone symposia
  • Place of Presentation: Keystone Resort (USA)
  • Year and Date: 2010-01-17
• [Presentation] New components of the primary processing pathway for Piwi-interacting piRNAs in Drosophila (2010)
  • Author(s): Kuniaki Saito
  • Organizer: Keystone symposia
  • Place of Presentation: Keystone Resort(USA)
  • Year and Date: 2010-01-17
• [Presentation] 新規mRNA由来piRNAの同定と大Maf因子traffic jamによるショウジョウバエPiwiの機能制御 (2009)
  • Author(s): 齋藤都暁
  • Organizer: 日本RNA学会
  • Place of Presentation: 朱鷺メッセ(新潟県)
  • Year and Date: 2009-07-28
• [Presentation] piRNAの世代間伝搬が担う遺伝シグナル (2009)
  • Author(s): 齋藤都暁
  • Organizer: 日本エピジェネティクス研究会
  • Place of Presentation: 学術総合センター(東京都)
  • Year and Date: 2009-05-22