Analysis of protein interaction networks by photo-cross-linking

• Project/Area Number: 21770195
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Molecular biology
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: SATO Aya The Institute of Physical and Chemical Research, 拡張遺伝暗号システム研究チーム, 特別研究員 (30469902)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2010: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2009: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
• Keywords: 蛋白質 / 光クロスリンク / 非天然型アミノ酸 / 分子間相互作用

Research Abstract

"The photo-cross-linking method" is the new technique that can stabilized protein complexes formed in living cells to isolate and analyze them. In this study, I identified the efficient positions to incorporate photo-cross-linkers in several protein-binding domains for the purpose of applying the photo-cross-linking method to the elucidation of the protein interaction networks.
Furthermore, I succeeded in the crystallographic study of the cross-linked protein complex.

Research Products

• [Journal Article] Genetic Incorporation of a Photo-Crosslinkable Amino Acid Reveals Novel Protein Complexes with GRB2 in Mammalian Cells. (2011)
  • Author(s): 樋野展正、尾山大明、佐藤文、向井崇人、伊良波史枝、林明子、秦裕子、山本雅、横山茂之、坂本健作
  • Journal Title: Journal of molecular biology 406 Pages: 343-353
  • Peer Reviewed
• [Journal Article] Crystallographic Study of a Site-Specifically Cross-Linked Protein Complex with a Genetically Incorporated Photoreactive Amino Acid. (2011)
  • Author(s): 佐藤心、三桝信哉、佐藤文、樋野展正、坂本健作、梅原崇史、横山茂之
  • Journal Title: Biochemistry 50 Pages: 250-257
  • Peer Reviewed
• [Journal Article] Genetic Incorporation of a Photo-Crosslinkable Amino Acid Reveals Novel Protein Complexes with GRB2 in Mammalian Cells. (2011)
  • Author(s): 樋野展正、尾山大明、佐藤文、向井崇人、伊良波史枝、林明子、秦裕子、山本雅、横山茂之、坂本健作
  • Journal Title: Journal of molecular biology Volume: 406 Pages: 343-353
  • Peer Reviewed
• [Journal Article] Crystallographic Study of a Site-Specifically Cross-Linked Protein Complex with a Genetically Incorporated Photoreactive Amino Acid. (2011)
  • Author(s): 佐藤心、三桝信哉、佐藤文、樋野展正、坂本健作、梅原崇史、横山茂之
  • Journal Title: Biochemistry Volume: 50 Pages: 250-257
  • Peer Reviewed
• [Journal Article] Codon reassignment in the Escherichia coli genetic code. (2010)
  • Author(s): 向井崇人、林明子、伊良波史枝、佐藤文、大竹和正、横山茂之、坂本健作
  • Journal Title: Nucleic Acids Research 38 Pages: 8188-8195
  • Peer Reviewed
• [Journal Article] Codon reassignment in the Escherichia coli genetic code. (2010)
  • Author(s): 向井崇人、林明子、伊良波史枝、佐藤文、大竹和正、横山茂之、坂本健作
  • Journal Title: Nucleic Acids Research Volume: 38 Pages: 8188-8195
  • Peer Reviewed
• [Presentation] 大腸菌におけるRF-1ノックアウトによるUAGコドンの再定義 (2010)
  • Author(s): 佐藤文、向井崇人、林明子、伊良波史枝、大竹和正、横山茂之、坂本健作
  • Organizer: 第5回無細胞生命科学研究会
  • Place of Presentation: 岡山県岡山市
• [Presentation] 大腸菌におけるRF-1ノックアウトによるUAGコドンの再定義 (2010)
  • Author(s): 佐藤文、向井崇人、林明子、伊良波史枝、大竹和正、横山茂之、坂本健作
  • Organizer: 第5回 無細胞生命科学研究会
  • Place of Presentation: 岡山市
• [Presentation] サプレッサーtRNAの改変による無細胞翻訳系における非天然型アミノ酸のタンパク質への導入効率の向上 (2009)
  • Author(s): 佐藤文、坂本健作、横山茂之、大野敏、横川隆志、西川一八
  • Organizer: 第4回無細胞生命科学研究会
  • Place of Presentation: 岐阜県下呂市
• [Presentation] サプレッサーtRNAの改変による無細胞翻訳系における非天然型アミノ酸のタンパク質への導入効率の向上 (2009)
  • Author(s): 佐藤文、坂本健作、横山茂之、大野敏、横川隆志、西川一八
  • Organizer: 第4回 無細胞生命科学研究会
  • Place of Presentation: 岐阜県下呂市
• [Remarks] ホームページ等
  • URL: http://protein.gsc.riken.jp/sakamoto/
• [Remarks]
  • URL: http://protein.gsc.riken.jp/sakamoto/