A novel activity of HIV-1 Rev protein to remodel export RNPs in directing virus-specific RNA export
| Project/Area Number |
21770204
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Kyoto University |
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Principal Investigator |
TANIGUCHI Ichiro Kyoto University, ウイルス研究所, 助教 (00467432)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2009: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | RNA核外輸送 / mRNAスプライシング / RNA-タンパク質複合体 / HIV-1 / Revタンパク質 / RNP / スプライシング / mRNA前駆体スプライシング |
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| Research Abstract |
HIV-1 Rev protein induces export of singly spliced HIV-1 transcript (4kb-RNA) that is normally retained in the nucleus. However, how 4kb-RNA is exported is not well understood, since such a transcript should have possibility of utilizing CRM1-dependent or TAP-dependent export, or both. To understand the export mechanism, we generated model RNA substrates that recapitulated the situation of export of 4kb-RNA in Xenopus oocytes, and investigated the action of Rev in detail. We found that such model RNA substrates utilized exclusively CRM1-dependent export pathway in the presence of Rev. Most importantly, this commitment to a single RNA export pathway is achieved by Rev's ability not only to induce CRM1-dependent export but also to suppress TAP-dependent export.
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Report
(3 results)
Research Products
(7 results)
