Study of the molecular mechanism of oxidative stress response induced by mitochondrial ROS

• Project/Area Number: 21770208
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Osaka University
• Principal Investigator: SHIBUYA Toshiharu Osaka University, 医学系研究科, 助教 (70448033)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2009: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
• Keywords: 酸化ストレス / ミトコンドリア / 細胞内情報伝達

Research Abstract

An experimental assay system was employed to generate excessive ROS in the mitochondria in a precisely controlled spatio-temporal manner. Using mammalian cells and worm C. elegans generating mitochondrial ROS, I analyzed the stress response and the molecular mechanism induced by mitochondrial ROS in vitro and in vivo. I revealed that factors involved in the mitochondrial permeability transition (Cyclophilin D and Ca++) were contributed to the mitochondrial oxidative stress response.

Research Products

• [Presentation] Cell death induced by mitochondrial ROS generated by photodynamic action of KillerRed protein (2010)
  • Author(s): 渋谷利治
  • Organizer: The 7th Conference of Asian Society for mitochondrial Research and Medicine
  • Place of Presentation: 福岡国際会議場(福岡市、福岡県)
  • Year and Date: 2010-12-16
• [Presentation] Cell death induced by mitochondrial ROS generated by photodynamic action of KillerRed protein (2010)
  • Author(s): 渋谷利治
  • Organizer: The 7^<th> Conference of Asian Society for mitochondrial Research and Medicine
  • Place of Presentation: 福岡国際会議場(福岡市、福岡県)
  • Year and Date: 2010-12-16
• [Presentation] Study of cell death induced by mitochondrial ROS generated by mtKillerRed protein (2010)
  • Author(s): 渋谷利治
  • Organizer: 第33回日本分子生物学会
  • Place of Presentation: 神戸ポートアイランド(神戸市、兵庫県)
  • Year and Date: 2010-12-10
• [Presentation] Study of cell death induced by photodynamic action of mitochondrial KillerRed protein (2010)
  • Author(s): 渋谷利治
  • Organizer: Gordon Research Conference (Cell Death)
  • Place of Presentation: Salve Regina Universityアメリカ合衆国(RI)
  • Year and Date: 2010-08-25
• [Remarks] ホームページ等
  • URL: http://www.med.osaka-u.ac.jp/pub/gene/www/laboratory/shibuya1.html