The analysis of membrane interface in the formation of the cell-cell adhesion complex
Project/Area Number |
21770209
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Cell biology
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Research Institution | Osaka University |
Principal Investigator |
YAMAZAKI Yuji Osaka University, 大学院・生命機能研究科, 助教 (80527664)
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Project Period (FY) |
2009 – 2010
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Project Status |
Completed (Fiscal Year 2010)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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Keywords | タイトジャンクション / シグナル / ZO-1 / アドヘレンスジャンクション / アクトミオシンリング / 上皮細胞シート / バリアー機能 / ZO1 / 2 / RhoGEF |
Research Abstract |
The epithelial cell-cell adhesion complex, which consists of belt-like adherens junctions (AJ) and tight junctions (TJ), is thought to be an important factor in many molecular events. The role of this complex comes from spatio-temporal regulation that leads to AJ and TJ formation, a process that is poorly understood. In this project, we analyzed the FRAP dynamics of claudins and ZO-1 to reveal their spatiotemporal behaviors and interactions in epithelial cells, and use these observations as the basis for a new scheme to describe their molecular interactions. We examined the spatiotemporal regulation of AJ/TJ formation and modulation, finding new spatiotemporal signaling for AJ regulation that includes the identification of Tara, a GEF binding protein, as an AJ protein.
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Report
(3 results)
Research Products
(20 results)
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[Journal Article] Tara up-regulates E-cadherin transcription by binding to theTrio RhoGEF and inhibiting Rac signaling.2011
Author(s)
Yano, T.#, Yamazaki, Y.#, Adachi, M., Okawa, K., Fort, P., Uji, M., Tsukita, S., Tsukita, S.^*
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Journal Title
J.Cell Biol. 193
Pages: 319-332
Related Report
Peer Reviewed
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[Journal Article] Predicted expansion of the claudin multigene family.2011
Author(s)
Mineta, K.^*, Yamamoto, Y., Yamazaki, Y.,Tanaka, H., Tada, Y., Saito, K., Tamura,A, Igarashi, M., Endo, T., Takeuchi, K., Tsukita, S.^*
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Journal Title
FEBS letters 585
Pages: 606-612
Related Report
Peer Reviewed
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[Journal Article] Loss of claudin-15, but not claudin-2, causes Na+ deficiency and Glucose malabsorption in mouse small intestine.2011
Author(s)
Tamura, A., Hayashi, H., Imasato, M., Yamazaki, Y., Hagiwara, A., Wada, M., Noda,T., Watanabe, M., Suzuki, Y., Tsukita, S.^*
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Journal Title
Gastroenterology 140
Pages: 913-923
Related Report
Peer Reviewed
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[Journal Article] Predicted expansion of the claudin multigene family.2011
Author(s)
Mineta, K., Yamamoto, Y., Yamazaki, Y., Tanaka, H., Tada, Y., Saito, K., Tamura, A., Igarashi, M., Endo, T., Takeuchi, K., Tsukita, Sa.
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Journal Title
FEBS letters
Volume: 585
Pages: 606-612
Related Report
Peer Reviewed
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[Journal Article] Loss of claudin-15, but not claudin-2, causes Na+ deficiency and glucose malabsorption in mouse small intestine.2011
Author(s)
Tamura A, Hayashi H, Imasato M, Yamazaki Y, Hagiwara A, Wada M, Noda T, Watanabe M, Suzuki Y, Tsukita S.
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Journal Title
Gastroenterology
Volume: 140
Pages: 913-923
Related Report
Peer Reviewed
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[Journal Article] Roles of ZO-1 and ZO-2 in establishment of the belt-like adherens and tight junctions with paracellular permselective barrier function.2009
Author(s)
Tsukita, S., Katsuno, T., Yamazaki, Y., Umeda, K., Tamura, A., Tsukita, S.
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Journal Title
Ann.N.Y.Acad.Sci. 1165
Pages: 44-52
Related Report
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