| Project/Area Number |
21770209
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | Osaka University |
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Principal Investigator |
YAMAZAKI Yuji Osaka University, 大学院・生命機能研究科, 助教 (80527664)
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| Project Period (FY) |
2009 – 2010
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| Project Status |
Completed (Fiscal Year 2010)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | タイトジャンクション / シグナル / ZO-1 / アドヘレンスジャンクション / アクトミオシンリング / 上皮細胞シート / バリアー機能 / ZO1 / 2 / RhoGEF |
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| Research Abstract |
The epithelial cell-cell adhesion complex, which consists of belt-like adherens junctions (AJ) and tight junctions (TJ), is thought to be an important factor in many molecular events. The role of this complex comes from spatio-temporal regulation that leads to AJ and TJ formation, a process that is poorly understood. In this project, we analyzed the FRAP dynamics of claudins and ZO-1 to reveal their spatiotemporal behaviors and interactions in epithelial cells, and use these observations as the basis for a new scheme to describe their molecular interactions. We examined the spatiotemporal regulation of AJ/TJ formation and modulation, finding new spatiotemporal signaling for AJ regulation that includes the identification of Tara, a GEF binding protein, as an AJ protein.
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