Analysis on regulatory mechanisms for endocytosis mediated by membrane-bending proteins

• Project/Area Number: 21770211
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Kobe University
• Principal Investigator: ITOH Toshiki Kobe University, 大学院・医学研究科, 准教授 (30313092)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 生体膜 / エンドサイトーシス / F-BARドメイン / Fer / チロシンキナーゼ / マクロピノサイトーシス / マクロピノサイト-シス

Research Abstract

Fer, a tyrosine kinase harboring membrane-bending F-BAR domain, was found to be activated in a manner dependent on membrane curvature. It was also revealed that Fer is involved in macropinocytosis mediated by membrane ruffle formation, as well as clathrin-mediated endocytosis for internalization of transferrin receptor.

Research Products

• [Journal Article] Proteome of acidic phospholipid-binding proteins : Spatial and temporal regulation of coronin 1A by phosphoinositides. (2010)
  • Author(s): Tsujita, K., Itoh, T., Kondo, A., Oyama, M., Kozuka-Hata, H., Irino, Y., Hasegawa, J., Takenawa, T.
  • Journal Title: J.Biol.Chem. 285(9) Pages: 6781-6789
• [Journal Article] Mutation of ARHGAP9 in patients with coronary spastic angina. (2010)
  • Author(s): Takefuji, M., Asano, H., Mori, K., Amano, M., Kato, K., Watanabe, T., Morita, Y., Katsumi, A., Itoh, T., Takenawa, T., Hirashiki, A., Izawa, H., Nagata, K., Hirayama, H., Takatsu, F., Naoe, T., Yokota, M., Kaibuchi, K.
  • Journal Title: J.Hum.Genet. 55(1) Pages: 42-49
• [Journal Article] Proteome of acidic phospholipid-binding proteins : Spatial and temporal regulation of coronin 1A by phosphoinositides. (2010)
  • Author(s): Tsujita, K., Itoh. T., Kondo, A., Oyama, M., Kozuka-Hata, H., Irino, Y., Hasegawa, J., Takenawa, T.
  • Journal Title: The Journal of Biological Chemistry 285 Pages: 6781-6789
  • Peer Reviewed
• [Journal Article] Dynamic interaction of amphiphysin with N-WASP regulates actin assembly. (2009)
  • Author(s): Yamada, H., Padilla-Parra, S., Park, S.J., Itoh, T., Chaineau, M., Monaldi, I., Cremona, O., Benfenati, F., De Camilli, P., Coppey-Moisan, M., Tramier, M., Galli, T. Takei, K.
  • Journal Title: J.Biol.Chem. 284(49) Pages: 34244-34256
  • NAID: 130004505697
• [Journal Article] The tyrosine kinase Fer is a downstream target of the PLD-PA pathway that regulates cell migration. (2009)
  • Author(s): Itoh, T.*, Hasegawa, J., Tsujita, K., Kanaho, Y. Takenawa, T.*
  • Journal Title: Sci.Signal. 2(87)
• [Journal Article] Dynamic interaction of amphiphysin with N-WASP regulates actin assembly. (2009)
  • Author(s): Yamada, H., Padilla-Parra, S., Park, S.J., Itoh. T., Chaineau, M., Monaldi, I., Cremona, O., Benfenati, F., De Camilli, P., Coppey-Moisan, M., Tramier, M., Galli, T., Takei, K.
  • Journal Title: The Journal of Biological Chemistry 284 Pages: 34244-34256
  • NAID: 130004505697
  • Peer Reviewed
• [Journal Article] The tyrosine kinase Fer is a downstream target of the PLD-PA pathway that regulates cell migration. (2009)
  • Author(s): Itoh. T., Hasegawa, J., Tsujita, K., Kanaho, Y., Takenawa, T.
  • Journal Title: Science Signaling 2(online journal)
  • Peer Reviewed
• [Presentation] International Symposium on Organelle Network : Microbiology, Immunology, and Cell Biology (2010)
  • Author(s): Toshiki Itoh
  • Organizer: Membrane sculpting by lipid-binding domains大阪大学医学部緒方洪庵生誕200周年記念事業グローバルCOEプログラム「オルガネラネットワーク医学創成プログラム」
  • Place of Presentation: 大阪国際会議場(グランキューブ大阪)特別会議場(日本、大阪市)
  • Year and Date: 2010-07-13
• [Presentation] 細胞内及び細胞外における膜ダイナミクス (2010)
  • Author(s): 伊藤俊樹、長谷川純矢、竹縄忠臣、滝口金吾、滝口陽子
  • Organizer: 第10回日本蛋白質科学会年会
  • Place of Presentation: 札幌コンベンションセンター(北海道)(招待講演)
  • Year and Date: 2010-06-18
• [Presentation] Functional domain modules that generate and/or sense membrane curvature (2010)
  • Author(s): Seminar、Toshiki Itoh
  • Organizer: Griffith University
  • Place of Presentation: Gold Coast, Australia
  • Year and Date: 2010-03-30
• [Presentation] Functional domain modules that generate and/or sense membrane curvature (2010)
  • Author(s): IBB Seminar、伊藤俊樹
  • Organizer: Pohang University of Science and Technology
  • Place of Presentation: 韓国、浦項
  • Year and Date: 2010-03-24
• [Presentation] 2010年6月18日5. International Symposium on Cellular Signaling-principles and functions- (2009)
  • Author(s): Toshiki Itoh
  • Organizer: Membrane curvature -dependent regulation of Fer tyrosine kinase
  • Place of Presentation: 筑波大学(日本、つくば市)
  • Year and Date: 2009-11-18
• [Presentation] Fer is a membrane curvature-dependent tyrosine kinase downstream of PLD-PA pathway essential for cell migration (2009)
  • Author(s): Toshiki Itoh, Junya Hasegawa, Tadaomi Takenawa.
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋国際会議場(ポスター発表)
  • Year and Date: 2009-06-04
• [Presentation] Fer is a membrane curvature-dependent tyrosine kinase downstream of PLD-PA pathway essential for cell migration (2009)
  • Author(s): Toshiki Itoh, Junya Hasegawa, Tadaomi Takenawa
  • Organizer: 第61回日本細胞生物学会大会
  • Place of Presentation: 名古屋国際会議場(愛知県)
  • Year and Date: 2009-06-04
• [Presentation] 脂質結合ドメインによる生体膜ダイナミクスの制御機構
  • Author(s): 伊藤俊樹、長谷川純矢、竹縄忠臣、滝口金吾、滝口陽子
  • Organizer: 第10回日本蛋白質科学会年会、ワークショップ「細胞内及び細胞外における膜ダイナミクス」
  • Place of Presentation: 札幌コンベンションセンター(口頭発表)
• [Book] 改訂タンパク質実験ハンドブック (2011)
  • Author(s): 竹縄忠臣、伊藤俊樹(共同編集)
  • Publisher: 羊土社
• [Book] 脂質二重層の形状を制御する分子機構実験医学増刊・分子から個体へと深化する脂質生物学(佐々木雄彦、横溝岳彦、竹縄忠臣編) (2010)
  • Author(s): 伊藤俊樹
  • Publisher: 羊土社
• [Remarks] ホームページ等
  • URL: http://www.med.kobe-u.ac.jp/membrane/index.html