Analysis of molecular mechanisms of the formation of the intercellular junction-underlying actin bundle using semi-intact epithelium.

• Project/Area Number: 21770212
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Cell biology
• Research Institution: Kobe University
• Principal Investigator: HIGASHI Tomohito Kobe University, 大学院・医学研究科, グローバルCOE研究員 (70515072)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 細胞間接着 / アクチン細胞骨格 / Rho低分子量Gタンパク質 / ArhGEF11 / 上皮細胞 / タイトジャンクション / RhoGEF / 裏打ちアクチン / RhoA

Research Abstract

Epithelial sheets play important roles in morphogenesis of multicellular organisms. So far, it has been unknown how the intercellular junction-underlying actin cytoskeleton are formed. In this study, I showed that ArhGEF11, a guanine nucleotide exchange factor of the small GTPase RhoA, is localized at the cell-cell-junction and that ArhGEF11 is required for the formation of the actin cytoskeleton vertical to cell-cell junction during the formation process of cell-cell junction.

Research Products

• [Journal Article] Horiuchi H Flightless-I (Fli-I) regulates the actin assembly of diaphanous-related formins (DRFs) Daam1 and mDia1 in cooperationwith active Rho GTPases. (2010)
  • Author(s): Higashi T, Ikeda T, Murakami T, Shirakawa R, Kawato M, Okawa K, Furuse M, Kimura T, Kita T.
  • Journal Title: J Biol Chem 285巻 Pages: 16231-8
  • Peer Reviewed
• [Presentation] Flightless-I (Fli-I) regulates the actin assembly activity of Diaphanous .related formins (DRFs) Daam1 and mDia1 in cooperation with active Rho GTPases (2010)
  • Organizer: アメリカ細胞生物学会
  • Place of Presentation: ペンシルベニア会議場(米国)
  • Year and Date: 2010-12-13
• [Presentation] Flightless-I (Fli-I) regulates the actin assembly activity of Diaphanous-related formins (DRFs) Daaml and mDial in cooperation with active Rho. (2010)
  • Author(s): 東智仁
  • Organizer: アメリカ細胞生物学会
  • Place of Presentation: ペンシルベニア会議場(米国・フィラデルフィア)
  • Year and Date: 2010-12-13
• [Presentation] PDZ-RhoGEF は細胞間接着部位に局在し上皮細胞のアピカル面張力を制御する (2010)
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 大阪国際会議場
  • Year and Date: 2010-05-19
• [Presentation] PDZ-RhoGEFは細胞間接着部位に局在し上皮細胞のアピカル面張力を制御する (2010)
  • Author(s): 東智仁
  • Organizer: 日本細胞生物学会
  • Place of Presentation: 大阪国際会議場(大阪府)
  • Year and Date: 2010-05-19
• [Presentation] ArhGEF11 localizes at intercellular junction of epithelial cells and regulates the actin cytoskeletal organization. (2009)
  • Author(s): 東智仁
  • Organizer: 日本分子生物学会第32回年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-10