Live imaging of protein transport through the Golgi apparatus

Research Project

Project/Area Number	21770215
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Cell biology
Research Institution	Kyushu University
Principal Investigator	TOKITA Kumi Kyushu University, 大学院・医学研究院, 学術研究員 (50415296)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000) Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000) Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Keywords	細胞構造・機能 / ゴルジ体
Research Abstract	The ER and Golgi are dynamic organelles that assemble and disassemble during the cell cycle. To elucidate the molecular mechanisms underlying these changes in organelle organization, I searched for novel factors that play a fundamental role in biogenesis and maintenance of the ER and Golgi, using live-imaging technique as a powerful tool. Using modified in vitro ER reformation assay technique and by biochemical approach, I identified a soluble factor sufficient for building and maintaining tubular ER lattice by itself.

Report

(3 results)