Elucidation of mechanisms for cell migration by visualization of PLD activity and monitoring of single molecule dynamics in living cells.

Research Project

Project/Area Number	21770223
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Cell biology
Research Institution	National Institute of Advanced Industrial Science and Technology
Principal Investigator	NAGASAKI Akira National Institute of Advanced Industrial Science and Technology, バイオメディカル研究部門, 研究員 (30392640)
Project Period (FY)	2009 – 2010
Project Status	Completed (Fiscal Year 2010)
Budget Amount *help	¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000) Fiscal Year 2010: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000) Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Keywords	細胞骨格 / 運動 / 細胞運動 / PLD / 全反射顕微鏡 / ホスファチジン酸
Research Abstract	Phospholipase D (PLD) is an enzyme that catalyzes the hydrolysis of a major component of membrane phospholipids, phosphatidylcholine, to generate a second messenger phosphatidic acid (PA). We have already shown that PLD gene is required for cell migration in NBT-II cells and Dictyostelium cells. To elucidate the role of PLD in migrating cells, we tried to construct probes for detection of PLD activity and to observe the single molecular dynamics of PLD on cell membrane.