| Project/Area Number |
21770223
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cell biology
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| Research Institution | National Institute of Advanced Industrial Science and Technology |
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Principal Investigator |
NAGASAKI Akira National Institute of Advanced Industrial Science and Technology, バイオメディカル研究部門, 研究員 (30392640)
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|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2010: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2009: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
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|---|
| Keywords | 細胞骨格 / 運動 / 細胞運動 / PLD / 全反射顕微鏡 / ホスファチジン酸 |
|---|
| Research Abstract |
Phospholipase D (PLD) is an enzyme that catalyzes the hydrolysis of a major component of membrane phospholipids, phosphatidylcholine, to generate a second messenger phosphatidic acid (PA). We have already shown that PLD gene is required for cell migration in NBT-II cells and Dictyostelium cells. To elucidate the role of PLD in migrating cells, we tried to construct probes for detection of PLD activity and to observe the single molecular dynamics of PLD on cell membrane.
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