Functional analysis of Kinesin superfamily protein in kidney development

• Project/Area Number: 21770232
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: Kumamoto University
• Principal Investigator: TERABAYASHI Takeshi Kumamoto University, 発生医学研究所・COEリサーチ・アソシエイト (40452429)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2009: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 腎臓発生 / キネシンスーパーファミリー蛋白質 / 細胞骨格 / 微小管 / 細胞接着 / キネシン / 細胞極性

Research Abstract

In this study, we performed the functional analysis of Kif26b, a member of kinesin super family proteins. Kif26b is expressed in the metanephric mesenchymes, a population of multipotent kidney progenitors. Disruption of Kif26b causes kidney agenesis because of impaired ureteric bud attraction. In the Kif26b-null metanephros, compact adhesion between mesenchymal cells adjacent to the ureteric buds and the polarized distribution of integrin alpha 8 were impaired, resulting in failed maintenance of Gdnf, a critical ureteric bud attractant. Overexpression of Kif26b in vitro caused increased cell adhesion through interactions with nonmuscle myosin. Thus, Kif26b is essential for kidney development because it regulates the adhesion of mesenchymal cells in contact with ureteric buds.

Research Products

• [Journal Article] Nucleoredoxin sustains Wnt/β-catenin signalling by retaining a pool of inactive Dishevelled protein. (2010)
  • Author(s): Funato Y., Terabayashi T., Sakamoto R., Okuzaki D., Ichise H., Nojima H., Yoshida N., Miki H.
  • Journal Title: Curr.Bio. 20 Pages: 1945-1952
  • Peer Reviewed
• [Journal Article] Kif26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme. (2010)
  • Author(s): Uchiyama Y., Sakaguchi Y., Terabayashi T., Inenaga T., Inoue S., Kobayashi C., Oshima N., Kiyonari H., Nakagata N.,Sato Y., Sekiguchi K., Miki H., Fujimura S.Tanaka S.S., Nishinakamura R.
  • Journal Title: Proc.Natl.Acad.Sci.USA 107 Pages: 9240-9245
  • Peer Reviewed
• [Journal Article] Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of Flightless-I to Myeloid differentiation primary response gene (88) (2010)
  • Author(s): Hayashi T., Funato Y., Terabayashi T., Morinaka A., Sakamoto R., Ichise H., Fukuda H., Yoshida N., Miki H.
  • Journal Title: J.Bio.Chem. 285 Pages: 18586-18593
  • Peer Reviewed
• [Journal Article] Par1b/MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF13B to regulate axon formation. (2010)
  • Author(s): Yoshimura Y., Terabayashi T., Miki H.
  • Journal Title: Mol.Cell.Bio. 30 Pages: 2206-2219
  • Peer Reviewed
• [Journal Article] Nucleoredoxin sustains Wnt/β-catenin signalling by retaining a pool of inactive Dishevelled protein. (2010)
  • Author(s): 船戸洋佑
  • Journal Title: Current Biology Volume: 20 Pages: 1945-1952
  • Peer Reviewed
• [Journal Article] Kif26b, a kinesin family gene, regulates adhesion of the embryonic kidney mesenchyme. (2010)
  • Author(s): 内山裕佳子
  • Journal Title: Proc.Natl.Acad.Sci.USA Volume: 107 Pages: 9240-9245
  • Peer Reviewed
• [Journal Article] Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of Flightless-I to Myeloid differentiation prinlary response gelle (88). (2010)
  • Author(s): 林達也
  • Journal Title: Journal of Biological Chemistry Volume: 285 Pages: 18586-18593
  • Peer Reviewed
• [Journal Article] Par1b/MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF 13B to regulate axon formation. (2010)
  • Author(s): 吉村祐太
  • Journal Title: Molecular Cellular Biology Volume: 30 Pages: 2206-2219
  • Peer Reviewed
• [Journal Article] Par1b/MARK2 phosphorylates kinesin-like motor protein GAKIN/KIF13B to regulate axon formation. (2010)
  • Author(s): 吉村祐太
  • Journal Title: Molecular Cellular Bioloby 30 Pages: 2206-2219
  • Peer Reviewed
• [Journal Article] A coated vesicle-associated kinase of 104 kDa (CVAK104) induces lysosomal degradation of Frizzled 5 (Fzd5). (2009)
  • Author(s): Terabayashi T., Funato Y., Fukuda M., Miki H.
  • Journal Title: J.Bio.Chem. 284 Pages: 26716-26724
  • Peer Reviewed
• [Journal Article] 腎臓の発生と再生綜合臨床 (2009)
  • Author(s): 寺林健、西中村隆一
  • Journal Title: Vol.58 (3) Pages: 1829-1831
• [Journal Article] A coated vesicle-associated kinase of 104kDa (CVAK104) induces lysosomal degradation of Frizzled 5 (Fzd5). (2009)
  • Author(s): 寺林健
  • Journal Title: Journal of Biological Chemistory 284 Pages: 26716-26724
  • Peer Reviewed
• [Journal Article] 腎臓の発生と再生 (2009)
  • Author(s): 寺林健
  • Journal Title: 綜合臨床 58 Pages: 1829-1831
• [Presentation] Sall1 is involved in maintenance of nephron progenitors. (2010)
  • Author(s): Terabayashi T., Nishinakamura R.
  • Organizer: The 43rd Annual Meeting for Japanese Society of Developmental Biologists
  • Place of Presentation: Kyoto International Conference Center, Kyoto
  • Year and Date: 2010-06-21
• [Presentation] Sall1 is involved in maintenance of nephron progenitors. (2010)
  • Author(s): 寺林健
  • Organizer: 日本発生生物学会
  • Place of Presentation: 京都国際会議場(京都)
  • Year and Date: 2010-06-21
• [Presentation] Sall1 is involved in maintenance of nephron progenitors through regulation of Six2. (2010)
  • Author(s): Terabayashi T., Shuji Inoue, Nishinakamura R.
  • Organizer: International Symposia on "Cell Fate Regulation by Kumamoto University COE and Academia Sinica
  • Place of Presentation: Academia Sinica, Taipei, Taiwan
  • Year and Date: 2010-04-09
• [Presentation] Sall1 is involved in maintenance of nephron progenitors through regulation of Six2. (2010)
  • Author(s): 寺林健
  • Organizer: International Symposia on "Cell Fate Regulation by Kumamoto University COE and Academia Sinica"
  • Place of Presentation: Academia Sinica(台北)台湾
  • Year and Date: 2010-04-09
• [Presentation] A coated vesicle-associated kinase of 104 kDa induces lysosomal degradation of Frizzled 5 (2009)
  • Author(s): Terabayashi T., Funato Y., Miki H.
  • Organizer: The American Society for Cell Biology, 49th Annual Meeting
  • Place of Presentation: San Diego Convention Center, San Diego, USA
  • Year and Date: 2009-12-07
• [Presentation] A coated vesicle-associated kinase of 104kDa induces lysosomal degradation of Frizzled 5. (2009)
  • Author(s): 寺林健
  • Organizer: The American Society for Cell Biology
  • Place of Presentation: San Diego Convention Center(アメリカ)
  • Year and Date: 2009-12-07
• [Presentation] Kif-k regulates the mesenchymal condensation on contact with ureteric buds in kidney development. (2009)
  • Author(s): 阪口雅司
  • Organizer: The American Society for Cell Biology
  • Place of Presentation: San Diego Convention Center(アメリカ)
  • Year and Date: 2009-12-07
• [Presentation] Sall1 is involved in maintenance of nephron progenitors through regulation of Six2. (2009)
  • Author(s): Terabayashi T., Shuji Inoue, Nishinakamura R.
  • Organizer: International Joint Symposium on "Cell Fate Regulation Research : Stemcells and organogenesis.
  • Place of Presentation: Kumamoto University, Kumamoto
  • Year and Date: 2009-11-26
• [Presentation] Sall1 is essential for maintenance of nephron progenitors. (2009)
  • Author(s): Terabayashi T., Shuji Inoue, Nishinakamura R.
  • Organizer: GCOE mini symposium by Kumamoto University.
  • Place of Presentation: Kumamoto University, Kumamoto
  • Year and Date: 2009-06-01
• [Remarks] ホームページ等
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/integrative_cell_biology/publications.html
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/integrative_cell_biology/publications.html
• [Remarks]
  • URL: http://www.imeg.kumamoto-u.ac.jp/divisions/integrative_cell_biology/