Analysis for developmental mechanisms of protrusion and outgrowth

• Project/Area Number: 21770239
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Developmental biology
• Research Institution: 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設) (2010); National Institutes of Natural Sciences Okazaki Research Facilities (2009)
• Principal Investigator: MIYAGAWA Shinichi 大学共同利用機関法人自然科学研究機構(岡崎共通研究施設), 岡崎統合バイオサイエンスセンター, 助教 (30404354)
• Project Period (FY): 2009 – 2010
• Project Status: Completed (Fiscal Year 2010)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 器官形成 / 外生殖器 / シグナリングセンター / 成長因子 / 生殖器官 / 発生 / ヘッジホッグ

Research Abstract

Embryonic appendicular structures, including the developing external genitalia, are suitable models to analyze the developmental mechanisms of organ protrusion and outgrowth. Although several studies have evaluated individual functions of different growth factors on appendicular growth, the coordinated function of signaling cascades is poorly understood. Our results provide new insights into the integration of growth factor signaling (i.e. hedgehog, Wnt and Fgfs) in the appendicular developmental programs regulating external genitalia development. We also demonstrated the interaction of such growth factors and sex steroid hormones (androgens) during later stage of external genital development.

Research Products

• [Journal Article] Molecular mechanisms of induction of persistent changes by estrogenic chemicals on female reproductive tracts and external genitalia. (2011)
  • Author(s): Miyagawa S, Sato M, Iguchi T.
  • Journal Title: J Steroid Biochem Mol Biol in press
  • Peer Reviewed
• [Journal Article] Essential roles of androgen signaling in Wolffian duct stabilization and epididymal cell differentiation. (2011)
  • Author(s): Murashima A, Miyagawa S, Ogino Y, Nishida-Fukuda H, Araki K, Matsumoto T, Kaneko T, Yoshinaga K, Yamamura KI, Kurita T, Kato S, Moon AM, Yamada G.
  • Journal Title: Endocrinology in press
  • Peer Reviewed
• [Journal Article] Genetic analysis of hedgehog signaling in ventral body wall development and the onset of omphalocele formation. (2011)
  • Author(s): Matsumaru D, Haraguchi R, Miyagawa S, Motoyama J, Nakagata N, Meijlink F, Yamada G.
  • Journal Title: PLoS One 20
  • Peer Reviewed
• [Journal Article] Molecular mechanisms of induction of persistent changes by estrogenic chemicals on female reproductive tracts and external genitalia (2011)
  • Author(s): Miyagawa, S., et al.
  • Journal Title: J Steroid Biochem Mol Biol. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Essential roles of androgen signaling in Wolffian duct stabilization and epididymal cell differentiation. (2011)
  • Author(s): Murashima, A, Miyagawa, S., et al.
  • Journal Title: Endocrinology Volume: (in press)
  • Peer Reviewed
• [Journal Article] Genetic analysis of hedgehog signaling in ventral body wall development and the onset of omphalocele formation. (2011)
  • Author(s): Matsumaru D, Haraguchi R, Miyagawa S., et al.
  • Journal Title: PLoS One Volume: 20
  • Peer Reviewed
• [Journal Article] Estrogen receptor ESR1 is indispensable for the induction of persistent vaginal change by neonatal 5alpha-dihydrotestosterone exposure in mice. (2010)
  • Author(s): Miyagawa S, Katsu Y, Ohta Y, Sudo T, Lubahn DB, Iguchi T.
  • Journal Title: Biol Reprod 82 Pages: 497-503
  • Peer Reviewed
• [Journal Article] Estrogen receptor ESR1 is indispensable for the induction of persistent vaginal change by neonatal 5alpha-dihydrotestosterone exposure in mice (2010)
  • Author(s): Miyagawa, S., et al.
  • Journal Title: Biol Reprod Volume: 82 Pages: 497-503
  • Peer Reviewed
• [Journal Article] Dosage dependent hedgehog signals integrated with Wnt/b-catenin regulate embryonic external genitalia formation as an appendicular program. (2009)
  • Author(s): Miyagawa S, Moon A, Haraguchi R, Inoue C, Harada M, Nakahara C, Suzuki K, Matsumaru D, Kaneko T, Matsuo, I, Yang L, Taketo MM, Iguchi T, Evans SM, Yamada G.
  • Journal Title: Development 136 Pages: 3969-3978
  • Peer Reviewed
• [Journal Article] Genetic integrations of the androgen and Wnt/beta-catenin pathways for the masculinization of external genitalia. (2009)
  • Author(s): Miyagawa S, Satoh Y, Haraguchi R, Suzuki K, Iguchi T, Taketo MM, Nakagata N, Matsumoto T, Takeyama K, Kato S, Yamada G.
  • Journal Title: Mol Endcrinol. 23 Pages: 871-880
  • Peer Reviewed
• [Journal Article] Dosage dependent hedgehog signals integrated with Wnt/β-catenin regulate embryonic external genitalia formation as an appendicular program. (2009)
  • Author(s): Miyagawa, S., et al.
  • Journal Title: Development 136 Pages: 3969-3978
  • Peer Reviewed
• [Journal Article] Genetic integrations of the androgen and Wnt/beta-catenin pathways for the masculinization of external genitalia. (2009)
  • Author(s): Miyagawa, S., et al.
  • Journal Title: Molecular Endocrinilogy 23 Pages: 871-880
  • Peer Reviewed
• [Presentation] Indispensable role of estrogen receptor a for induction of persistent vaginal change by neonatal androgen exposure (2010)
  • Author(s): Miyagawa, S., et al
  • Organizer: 日本内分泌攪乱化学物質学会第13回研究発表会
  • Place of Presentation: 東京大学(東京都)
  • Year and Date: 2010-12-17
• [Presentation] 外生殖器雄性化過程におけるヘッジホッグシグナルの解析 (2010)
  • Author(s): 宮川信一、原口竜摩、井口泰泉、山田源
  • Organizer: 第29回日本アンドロロジー学会学術大会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-30
• [Presentation] 外生殖器雄性化過程におけるヘッジホッグシグナルの解析 (2010)
  • Author(s): Miyagawa, S., et al
  • Organizer: 第29回日本アンドロロジー学会学術大会
  • Place of Presentation: 日本獣医生命科学大学(東京都)
  • Year and Date: 2010-07-30
• [Presentation] Estrogen receptor a is indispensable for the induction of persistent vaginal change by neonatal 5a-dihydrotestosterone exposure. (2009)
  • Author(s): Miyagawa S., Katsu Y., Iguchi T.
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-10
• [Presentation] Estrogen receptor a is indispensable for the induction of persistent vaginal change by neonatal 5α-dihydrotestosterone exposure (2009)
  • Author(s): 宮川信一
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2009-12-10
• [Remarks] ホームページ
  • URL: http://www.nibb.ac.jp/%7Ebioenv1/index-j.html