| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Research Abstract |
We succeeded in making the conditional Nucleostemin deficient embryonic stem cells by a consecutive targeting of two times. Upon deletion of Nucleostemin allele, stop of proliferation and cell death had found. Moreover, it was clarified that the genes that did an important working to the maintenance of the undifferentiation of the embryonic stem cell, such as Oct3/4, Nanog, and Sox2 decreased along with the Nucleostemin loss. The possibility of the inducement or maintaining of the factors that Nucleostemin is related to the above-mentioned undifferentiated maintenance is suggested from this. On the other hand when Nucleostemin was over-expressed, the up-regulation of pluripotency associated genes was observed.
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