| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Research Abstract |
Three medaka (Oryzias latipes) mutations, kamigamo, shimogamo, and naruto, affecting the migration of primordial germ cells (PGCs) were analyzed in this study. Responsible genes of the kamigamo and shimogamo mutations are known as causative genes of human genetic diseases. The gene responsible for the naruto mutation was CPSF6 required for 3' RNA cleavage and polyadenylation processing. 3'RACE showed sdf1 mRNAs with short 3'UTR were produced in the mutant embryos. Whole mount in situ hybridization showed the expression of sdf1 in the mutant somite is longer to the anterior side than in the wild type. The short 3'UTR of sdf1 may be prevented from performing posttranscriptional degeneration that is mediated by microRNAs in the anterior somites. This should disturb the gradient of sdf1 to the posterior positioned presumptive gonad-area. This will cause defects in posterior migration of PGCs in the mutant embryos.
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