Genetic dissection of early development of primordial germ cells using medaka (Oryzias latipes) mutants.
Project/Area Number |
21770245
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Developmental biology
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Research Institution | National Institute for Basic Biology |
Principal Investigator |
SASADO Takao 基礎生物学研究所, バイオリソース研究室, 特別協力研究員 (90511204)
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Project Period (FY) |
2009-04-01 – 2013-03-31
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Project Status |
Completed (Fiscal Year 2013)
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Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2009: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | 始原生殖細胞 / 初期発生 / 細胞移動 / 小型魚類 / メダカ / 疾患モデル / ケモカイン / 遺伝子発現制御 / 発生生物学 / miRNA / microRNA / 生殖細胞 |
Research Abstract |
Three medaka (Oryzias latipes) mutations, kamigamo, shimogamo, and naruto, affecting the migration of primordial germ cells (PGCs) were analyzed in this study. Responsible genes of the kamigamo and shimogamo mutations are known as causative genes of human genetic diseases. The gene responsible for the naruto mutation was CPSF6 required for 3' RNA cleavage and polyadenylation processing. 3'RACE showed sdf1 mRNAs with short 3'UTR were produced in the mutant embryos. Whole mount in situ hybridization showed the expression of sdf1 in the mutant somite is longer to the anterior side than in the wild type. The short 3'UTR of sdf1 may be prevented from performing posttranscriptional degeneration that is mediated by microRNAs in the anterior somites. This should disturb the gradient of sdf1 to the posterior positioned presumptive gonad-area. This will cause defects in posterior migration of PGCs in the mutant embryos.
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Report
(5 results)
Research Products
(51 results)
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[Journal Article] The National BioResource Project Medaka (NBRP Medaka) : An Integrated Bioresource for Biological and Biomedical Sciences2010
Author(s)
Sasado, T ., Tanaka, M., Kobayashi, K., Sato, T ., Sakaizumi, M. and Naruse, K
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Journal Title
Exp. Anim
Volume: 59
Pages: 13-24
NAID
Related Report
Peer Reviewed
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[Journal Article] The National BioResource Project Medaka(NBRP Medaka): An Integrated Bioresource for Biological and Biomedical Sciences2010
Author(s)
Sasado, T., Tanaka, M., Kobayashi, K., Sato, T., Sakaizumi, M., Naruse, K.
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Journal Title
Experimental Animals Vol.59
Pages: 13-23
NAID
Related Report
Peer Reviewed
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[Presentation] Production of donor-derived offspring by allogenic transplantation of vitrified germ cells in medaka2013
Author(s)
Seki, S., Lee, S., Iwasaki, Y ., Y agisawa, M., Hiratsuka, T ., Kusano, K., Miwa, M., Endo, S., Ishida, M., Sasado, T ., Naruse, K., Y oshizaki, G
Organizer
The 50th Annual Meeting of the Society for Cryobiology
Place of Presentation
North Bethesda, U.S.A
Related Report
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[Presentation] Production of donor-derived offspring by allogenic transplantation of vitrified germ cells in medaka2013
Author(s)
Seki, S., Lee, S., Iwasaki, Y., Yagisawa, M., Hiratsuka, T., Kusano, K., Miwa, M., Endo, S., Ishida, M., Sasado, T., Naruse, K., Yoshizaki, G.
Organizer
The 50th Annual Meeting of the Society for Cryobiology
Place of Presentation
Bethesda North Marriott Hotel & Conference Center, Bethesda, Maryland, USA
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[Book] Medaka genomics and the methods and resources for decoding genomic functions. In Denny P., Kole C., ed., Genome Mapping and Genomics in Laboratory Animals2012
Author(s)
Kimura, T., Kamei Y., Takahna Y., Sasado, T., Naruse, K
Publisher
Springer
Related Report
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[Book] In Denny P., Kole C., ed., Genome Mapping and Genomics in Laboratory Animals2012
Author(s)
Kimura, T., Kamei Y., Takahna Y., Sasado, T., Naruse, K.
Total Pages
261
Publisher
Springer, Berlin, Germany
Related Report
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[Book] Cryopreservation of medaka sperm. In Kinoshita, M., Murata, K., Naruse, K.and Tanaka, M., ed., Medaka - Biology, Management, and Experimental Protocols2009
Author(s)
Sasado, T
Publisher
Wiley-Blackwell
Related Report
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[Book] Cryopreservation of medaka sperm.(In Kinoshita, M., Murata, K., and Naruse, K., Tanaka, M., ed., Medaka-Biology, Management, and Experimental Protocols)2009
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[Book] Artificial insemination using frozen medaka sperm.(In Kinoshita, M., Murata, K., Naruse, K.and Tanaka, M., ed., Medaka-Biology, Management, and Experimental Protocols)2009
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