The role of sumoylation in retinal progenitor proliferation
| Project/Area Number |
21770246
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Developmental biology
|
|---|
| Research Institution | Osaka Bioscience Institute |
|---|
Principal Investigator |
TERADA Koji Osaka Bioscience Institute, 発生生物学部門, 研究員 (70342722)
|
|---|
| Project Period (FY) |
2009 – 2010
|
| Project Status |
Completed (Fiscal Year 2010)
|
| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2009: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
|
|---|
| Keywords | Ubc9 / sumo / Xenopus / retina / cell cycle / progenitor / 網膜 / ubc9 / hmgb3 / アフリカツメガエル / 細胞増殖 |
|---|
| Research Abstract |
Precisely controlled progenitor proliferation is essential for normal development. However, molecular mechanisms, which control the correct timing of cell cycle withdrawal during development, have been poorly understood. We show here that ubc9, a sumo-conjugating enzyme, controls the cell cycle exit of retinal progenitors. We found that ubc9 is highly expressed in retinal progenitors and stem cells in Xenopus embryos. Ubc9 physically and functionally associates with Xenopus hmgb3, which is required for retinal cell proliferation, and prolonged expression of ubc9 and hmgb3 results in suppression of the cell cycle exit of retinal progenitors in a sumoylation-dependent manner. Overexpression of ubc9 and hmgb3 decreased expression of the cell-cycle inhibitor p27^<xic1>. Furthermore, progenitor proliferation is regulated, at least in part, by sumoylation of transcription factor Sp1. These results suggest a significant role of sumoylation for cell cycle regulation in retinal progenitors
|
Report
(3 results)
Research Products
(12 results)
-
[Journal Article] SMOC1 Is Essential for Ocular and Limb Development in Humans and Mice.2011
Author(s)
Okada I., Hamanoue H., Terada K., Tohma T., Megarbane A., Chouery E., Abou-Ghoch J., Jalkh N., Cogulu O., Ozkinay F., Horie K., Takeda J., Furuichi T., Ikegawa S., Nishiyama K., Miyatake S., Nishimura A., Mizuguchi T., Niikawa N., Hirahara F., Kaname T., Yoshiura K., Tsurusaki Y., Doi H., Miyake N., Furukawa T., Matsumoto N., Saitsu H.
-
Journal Title
Am.J.Hum.Genet. 88
Pages: 30-40
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
-
-
-
